NaCl enhances cellular cAMP and upregulates genes related to heterocyst development in the cyanobacterium, Anabaena sp. strain PCC 7120
Masahiko Imashimizu
Department of Life Sciences, The University of Tokyo, 381 Komaba, Meguro, Tokyo 153-8902, Japan
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 111 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Search for more papers by this authorHidehisa Yoshimura
Center of Systems Biology and Ecology, Faculty of Science, Toho University, 2–2–1 Miyama, Funabashi, Chiba 274-8510, Japan
Search for more papers by this authorHiroshi Katoh
Department of Life Sciences, The University of Tokyo, 381 Komaba, Meguro, Tokyo 153-8902, Japan
Division of Plant Functional Genomics, Life Science Research Center, Mie University, 1515, Kamihama, Tsu, Mie 514-8507, Japan.
Search for more papers by this authorShigeki Ehira
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, 255 Shimo-Ohkubo, Sakura, Saitama 338-8570, Japan
Search for more papers by this authorCorresponding Author
Masayuki Ohmori
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, 255 Shimo-Ohkubo, Sakura, Saitama 338-8570, Japan
*Corresponding author. Tel.: +81 48 858 3402; fax: +81 48 858 3384., E-mail address: [email protected]Search for more papers by this authorMasahiko Imashimizu
Department of Life Sciences, The University of Tokyo, 381 Komaba, Meguro, Tokyo 153-8902, Japan
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 111 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Search for more papers by this authorHidehisa Yoshimura
Center of Systems Biology and Ecology, Faculty of Science, Toho University, 2–2–1 Miyama, Funabashi, Chiba 274-8510, Japan
Search for more papers by this authorHiroshi Katoh
Department of Life Sciences, The University of Tokyo, 381 Komaba, Meguro, Tokyo 153-8902, Japan
Division of Plant Functional Genomics, Life Science Research Center, Mie University, 1515, Kamihama, Tsu, Mie 514-8507, Japan.
Search for more papers by this authorShigeki Ehira
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, 255 Shimo-Ohkubo, Sakura, Saitama 338-8570, Japan
Search for more papers by this authorCorresponding Author
Masayuki Ohmori
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, 255 Shimo-Ohkubo, Sakura, Saitama 338-8570, Japan
*Corresponding author. Tel.: +81 48 858 3402; fax: +81 48 858 3384., E-mail address: [email protected]Search for more papers by this authorEdited by K. Forchhammer
Abstract
Cellular cAMP was rapidly increased in the nitrogen-fixing cyanobacterium, Anabaena sp. PCC 7120, by the addition of 200 mM NaCl to the culture medium. Other alkaline-metal chlorides such as KCl or LiCl caused a lesser increase. The increase in cellular cAMP was transient and diminished when an adenylate cyclase, CyaC, which contains the conserved domains of the bacterial two-component regulatory system, was disrupted. DNA microarray analysis showed that expression of a gene cluster containing all5347 and alr5351 (hglE) was upregulated by NaCl in the wild-type strain but not in the cyaC mutant. Primer extension analysis indicated that transcription levels of all5347 and hglE were rapidly increased in response to the NaCl addition, and that these genes have NaCl-dependent transcription start sites. It was concluded that NaCl induced expression of genes related to heterocyst envelope formation in this cyanobacterium, possibly via a CyaC–cAMP signal transduction system.
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