The HPr(Ser) kinase of Streptococcus salivarius: a hexameric bifunctional enzyme controlled by glycolytic intermediates and inorganic phosphate

Phosphorylation of HPr, the small phosphocarrier protein of the phosphoenolpyruvate:sugar phosphotransferase system, on Ser46 by the HPr(Ser) kinase (HPrK/P) is a vital step in catabolite repression in Gram-positive bacteria. Streptococcus salivarius HPrK/P is reported to be a multimeric protein not regulated by metabolic intermediates. We re-evaluated the molecular mass of S. salivarius HPrK/P using sedimentation equilibrium ultracentrifugation, demonstrated that S. salivarius HPrK/P dephosphorylated HPr(Ser-P) and further characterised the effect of fructose 1,6-bisphosphate and other metabolic intermediates on enzyme activities. The molecular mass of S. salivarius HPrK/P was 201 305 Da, suggesting that streptococcal HPrK/P was a hexameric protein. Fructose 1,6-bisphosphate poorly activated streptococcal HPrK/P but protected kinase activity against inhibition by inorganic phosphate and inhibited dephosphorylation of HPr(Ser-P). Phosphoenolpyruvate and 2-phosphoglycerate, but not fructose 1-P, fructose 6-P, and ribulose 1,5-bisphosphate, also protected kinase activity against inhibition by inorganic phosphate. Thus, unlike previous reports, we show that fructose 1,6-bisphosphate and other key glycolytic intermediates played a pivotal role as a modulator of streptococcal HPrK/P activities.