In situ cross-linkable hyaluronan hydrogel enhances chondrogenesis
Corresponding Author
Cecilia Aulin
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden.Search for more papers by this authorKristoffer Bergman
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden
Search for more papers by this authorMarianne Jensen-Waern
Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden
Search for more papers by this authorPatricia Hedenqvist
Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden
Search for more papers by this authorJöns Hilborn
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden
Search for more papers by this authorThomas Engstrand
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden
Stockholm Craniofacial Centre, Department of Reconstructive Plastic Surgery, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Search for more papers by this authorCorresponding Author
Cecilia Aulin
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden.Search for more papers by this authorKristoffer Bergman
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden
Search for more papers by this authorMarianne Jensen-Waern
Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden
Search for more papers by this authorPatricia Hedenqvist
Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden
Search for more papers by this authorJöns Hilborn
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden
Search for more papers by this authorThomas Engstrand
Department of Materials Chemistry, Division of Polymer Chemistry, Uppsala University, SE-751 21 Uppsala, Sweden
Stockholm Craniofacial Centre, Department of Reconstructive Plastic Surgery, Karolinska University Hospital, SE-171 76 Stockholm, Sweden
Search for more papers by this authorAbstract
The present work describes the feasibility of a cross-linkable injectable hyaluronan hydrogel for cartilage repair. The hydrogel used is a two-component system based on aldehyde-modified hyaluronan and hydrazide-modified polyvinyl alcohol, which are rapidly cross-linked in situ upon mixing. The in vitro study showed that chondrocytes and mesenchymal cells cultured in the gel form cartilage-like tissue, rich in glycosaminoglycans, collagen type II and aggrecan. In a rabbit animal model the injection of the hydrogel improved the healing of a full-thickness cartilage defect created in the knee as compared to non-treated controls. This rabbit study showed that the regenerated cartilage defects stained more intensely for type II collagen upon treatment with the hydrogel. The hyaluronan-based hydrogel may be used as a delivery vehicle for both growth factors and/or cells for cartilage repair. The in vivo study also indicated that the hydrogel alone has a beneficial effect on cartilage regeneration. Copyright © 2011 John Wiley & Sons, Ltd.
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