Probing cell metabolism on insulin like growth factor(IGF)-1/tumor necrosis factor(TNF)-α and chargeable polymers co-immobilized conjugates
Rong You
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorLanqing Wang
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Rong You and Lanqing Wang contributed equally to this work and should be considered co-first authors.
Search for more papers by this authorLi Liu
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorYuanjian Wang
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorKaibin Han
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorHaiting Lin
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorYibei Wang
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorDaniel Raftery
University of Washington, Seattle, Washington, USA
Search for more papers by this authorCorresponding Author
Yan-Qing Guan
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou 510631, China
Correspondence
Yan-Qing Guan, School of Life Science, South China Normal University, Guangzhou 510631, China.
Email: [email protected]
Search for more papers by this authorRong You
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorLanqing Wang
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Rong You and Lanqing Wang contributed equally to this work and should be considered co-first authors.
Search for more papers by this authorLi Liu
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorYuanjian Wang
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorKaibin Han
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorHaiting Lin
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorYibei Wang
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Search for more papers by this authorDaniel Raftery
University of Washington, Seattle, Washington, USA
Search for more papers by this authorCorresponding Author
Yan-Qing Guan
School of Life Science, South China Normal University, Guangzhou, China
South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital, Guangzhou, China
Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou 510631, China
Correspondence
Yan-Qing Guan, School of Life Science, South China Normal University, Guangzhou 510631, China.
Email: [email protected]
Search for more papers by this authorAbstract
Cell culturing on different synthetic biomaterials would reprogram cell metabolism for adaption to their living conditions because such alterations in cell metabolism were necessary for cellular functions on them. Here we used metabolomics to uncover metabolic changes when liver cells were cultured on insulin-like growth factor (IGF)/tumor necrosis factor-α (TNF-α) and chargeable polymers co-modified biomaterials with the aim to explain their modulating effects on cell metabolism. The results showed that cell metabolism on IGF-1/TNF-α co-immobilized conjugates was significantly regulated according to their scatterings on the score plot of principal component analysis. Specifically, cell metabolisms were reprogrammed to the higher level of pyrimidine metabolism, β-alanine metabolism, and pantothenate and CoA biosynthesis, and the lower level of methionine salvage pathway in order to promote cell growth on IGF/TNF-α co-modified surface. Furthermore, cell senescence on PSt-PAAm-IGF/TNF-α surface was delayed through the regulation of branch amino acid metabolism and AMPK signal pathway. The research showed that metabolomics had great potential to uncover the molecular interaction between biomaterials and seeded cells, and provide the insights about cell metabolic reprogramming on IGF/TNF-α co-modified conjugates for cell growth.
CONFLICT OF INTEREST
The authors declare no conflicts of interest.
Supporting Information
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