Different degradation rates of nanofiber vascular grafts in small and large animal models
Takuma Fukunishi
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorChin Siang Ong
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorPooja Yesantharao
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorCameron A. Best
Center for Regenerative Medicine, Nationwide Children's Hospital, Columbus, OH
Search for more papers by this authorTai Yi
Center for Regenerative Medicine, Nationwide Children's Hospital, Columbus, OH
Search for more papers by this authorHuaitao Zhang
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorGunnar Mattson
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorJoseph Boktor
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorToshiharu Shinoka
Center for Regenerative Medicine, Nationwide Children's Hospital, Columbus, OH
Search for more papers by this authorChristopher K. Breuer
Center for Regenerative Medicine, Nationwide Children's Hospital, Columbus, OH
Search for more papers by this authorCorresponding Author
Narutoshi Hibino
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Correspondence
Narutoshi Hibino, Associate Professor of Surgery, Section of Cardiac Surgery, Department of Surgery, The University of Chicago Advocate Children's Hospital, 5841 S. Maryland Ave, Room E500B|MC5040, Chicago, IL 60637.
Email: [email protected]
Search for more papers by this authorTakuma Fukunishi
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorChin Siang Ong
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorPooja Yesantharao
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorCameron A. Best
Center for Regenerative Medicine, Nationwide Children's Hospital, Columbus, OH
Search for more papers by this authorTai Yi
Center for Regenerative Medicine, Nationwide Children's Hospital, Columbus, OH
Search for more papers by this authorHuaitao Zhang
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorGunnar Mattson
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorJoseph Boktor
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Search for more papers by this authorToshiharu Shinoka
Center for Regenerative Medicine, Nationwide Children's Hospital, Columbus, OH
Search for more papers by this authorChristopher K. Breuer
Center for Regenerative Medicine, Nationwide Children's Hospital, Columbus, OH
Search for more papers by this authorCorresponding Author
Narutoshi Hibino
Division of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, MD
Correspondence
Narutoshi Hibino, Associate Professor of Surgery, Section of Cardiac Surgery, Department of Surgery, The University of Chicago Advocate Children's Hospital, 5841 S. Maryland Ave, Room E500B|MC5040, Chicago, IL 60637.
Email: [email protected]
Search for more papers by this authorAbstract
Nanofiber vascular grafts have been shown to create neovessels made of autologous tissue, by in vivo scaffold biodegradation over time. However, many studies on graft materials and biodegradation have been conducted in vitro or in small animal models, instead of large animal models, which demonstrate different degradation profiles. In this study, we compared the degradation profiles of nanofiber vascular grafts in a rat model and a sheep model, while controlling for the type of graft material, the duration of implantation, fabrication method, type of circulation (arterial/venous), and type of surgery (interposition graft). We found that there was significantly less remaining scaffold (i.e., faster degradation) in nanofiber vascular grafts implanted in the sheep model compared with the rat model, in both the arterial and the venous circulations, at 6 months postimplantation. In addition, there was more extracellular matrix deposition, more elastin formation, more mature collagen, and no calcification in the sheep model compared with the rat model. In conclusion, studies comparing degradation of vascular grafts in large and small animal models remain limited. For clinical translation of nanofiber vascular grafts, it is important to understand these differences.
CONFLICT OF INTEREST
Drs. Breuer and Shinoka receive research support from Gunze Ltd. (Kyoto, Japan) and Cook Regentec (Indianapolis, IN). Dr. Breuer is on the Scientific Advisory Board of Cook Medical (Bloomington, IN). Dr. Hibino receives research support from Secant Medical (Telford, PA). Jed Johnson is a co-founder of Nanofiber Solutions, Inc. (Hilliard, OH). Cameron Best and Dr. Breuer are co-founders of LYST Therapeutics, LLC (Columbus, OH). The remaining authors have no conflicts of interest to disclose.
Supporting Information
| Filename | Description |
|---|---|
| TERM2977 supp-0001-FigureS1.TIFTIFF image, 985.7 KB |
Figure S1: Nanofiber vascular graft luminal diameter over time by ultrasonography The luminal diameters (mm) of PCL/CS vascular grafts are plotted in gray and the luminal diameters (mm) of PGA/PLCL vascular grafts are plotted in black. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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