Decursin in Angelica gigas Nakai (AGN) Enhances Doxorubicin Chemosensitivity in NCI/ADR-RES Ovarian Cancer Cells via Inhibition of P-glycoprotein Expression
Hyeong Sim Choi
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
These two authors contributed equally and should be considered co-first authors.Search for more papers by this authorSung-Gook Cho
Department of Biotechnology, Korea National University of Transportation, 61 University Rd, Jeungpyeong, Chungbuk, 368-701 Korea
These two authors contributed equally and should be considered co-first authors.Search for more papers by this authorMin Kyoung Kim
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Search for more papers by this authorMin Soo Kim
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Search for more papers by this authorSeung Hee Moon
Department of Applied Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Search for more papers by this authorIl Hwan Kim
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Search for more papers by this authorCorresponding Author
Seong-Gyu Ko
Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Correspondence to: Correspondence to: Seong-Gyu Ko, Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, 1 Hoegi, Seoul 130-701, Korea.
E-mail: [email protected]
Search for more papers by this authorHyeong Sim Choi
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
These two authors contributed equally and should be considered co-first authors.Search for more papers by this authorSung-Gook Cho
Department of Biotechnology, Korea National University of Transportation, 61 University Rd, Jeungpyeong, Chungbuk, 368-701 Korea
These two authors contributed equally and should be considered co-first authors.Search for more papers by this authorMin Kyoung Kim
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Search for more papers by this authorMin Soo Kim
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Search for more papers by this authorSeung Hee Moon
Department of Applied Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Search for more papers by this authorIl Hwan Kim
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Search for more papers by this authorCorresponding Author
Seong-Gyu Ko
Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, 1 Hoegi, Seoul, 130-701 Korea
Correspondence to: Correspondence to: Seong-Gyu Ko, Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, 1 Hoegi, Seoul 130-701, Korea.
E-mail: [email protected]
Search for more papers by this authorAbstract
Angelica gigas Nakai (AGN, Korean Dang-gui) is traditionally used for the treatment of various diseases including cancer. Here, we investigated multidrug-resistant phenotype-reversal activities of AGN and its compounds (decursin, ferulic acid, and nodakenin) in doxorubicin-resistant NCI/ADR-RES ovarian cancer cells. Our results showed that a combination of doxorubicin with either AGN or decursin inhibited a proliferation of NCI/ADR-RES cells. These combinations increased the number of cells at sub-G1 phase when cells were stained with Annexin V-fluorescein isothiocyanate. We also found that these combinations activated caspase-9, caspase-8, and caspase-3 and increased cleaved PARP level. Moreover, an inhibition of P-glycoprotein expression by either AGN or decursin resulted in a reduction of its activity in NCI/ADR-RES cells. Therefore, our data demonstrate that decursin in AGN inhibits doxorubicin-resistant ovarian cancer cell proliferation and induces apoptosis in the presence of doxorubicin via blocking P-glycoprotein expression. Therefore, AGN would be a potentially novel treatment option for multidrug-resistant tumors by sensitizing to anticancer agents. Copyright © 2016 John Wiley & Sons, Ltd.
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