Curcumin Suppresses MAPK Pathways to Reverse Tobacco Smoke-induced Gastric Epithelial–Mesenchymal Transition in Mice
Zhaofeng Liang
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorRui Wu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorWei Xie
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorHao Geng
Department of Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, 230032 China
Search for more papers by this authorLi Zhao
Department of Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, 230032 China
Search for more papers by this authorChunfeng Xie
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorJieshu Wu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorShanshan Geng
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorXiaoting Li
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorMingming Zhu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorWeiwei Zhu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorJianyun Zhu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorCong Huang
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorXiao Ma
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorCorresponding Author
Caiyun Zhong
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Correspondence to: Caiyun Zhong, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, 818 East Tianyuan Rd, Jiangning, Nanjing 211166, China; Hongyu Han, Department of Clinical Nutrition, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
E-mail: [email protected] (Caiyun Zhong); [email protected] (Hongyu Han)
Search for more papers by this authorCorresponding Author
Hongyu Han
Department of Clinical Nutrition, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060 China
Correspondence to: Caiyun Zhong, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, 818 East Tianyuan Rd, Jiangning, Nanjing 211166, China; Hongyu Han, Department of Clinical Nutrition, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
E-mail: [email protected] (Caiyun Zhong); [email protected] (Hongyu Han)
Search for more papers by this authorZhaofeng Liang
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorRui Wu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorWei Xie
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorHao Geng
Department of Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, 230032 China
Search for more papers by this authorLi Zhao
Department of Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, 230032 China
Search for more papers by this authorChunfeng Xie
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorJieshu Wu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorShanshan Geng
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorXiaoting Li
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorMingming Zhu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorWeiwei Zhu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorJianyun Zhu
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorCong Huang
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorXiao Ma
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Search for more papers by this authorCorresponding Author
Caiyun Zhong
Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing, 211166 China
Correspondence to: Caiyun Zhong, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, 818 East Tianyuan Rd, Jiangning, Nanjing 211166, China; Hongyu Han, Department of Clinical Nutrition, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
E-mail: [email protected] (Caiyun Zhong); [email protected] (Hongyu Han)
Search for more papers by this authorCorresponding Author
Hongyu Han
Department of Clinical Nutrition, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060 China
Correspondence to: Caiyun Zhong, Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, 818 East Tianyuan Rd, Jiangning, Nanjing 211166, China; Hongyu Han, Department of Clinical Nutrition, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
E-mail: [email protected] (Caiyun Zhong); [email protected] (Hongyu Han)
Search for more papers by this authorAbstract
Tobacco smoke (TS) has been shown to cause gastric cancer. Epithelial–mesenchymal transition (EMT) is a crucial pathophysiological process in cancer development. Mitogen-activated protein kinase (MAPK) pathways play central roles in tumorigenesis including EMT process. Curcumin is a promising chemopreventive agent for several types of cancers. In the present study, we investigated the effects of TS on MAPK pathway activation and EMT alterations in the stomach of mice, and the preventive effect of curcumin was further examined. Results showed that exposure of mice to TS for 12 weeks resulted in activation of extracellular regulated protein kinases 1 and 2 (ERK1/2), the Jun N-terminal kinase (JNK), p38, and ERK5 MAPK pathways as well as activator protein 1 (AP-1) proteins in stomach. TS reduced the mRNA and protein expression levels of the epithelial markers E-cadherin and ZO-1, while the mRNA and protein expression levels of the mesenchymal markers vimentin and N-cadherin were increased. Treatment of curcumin effectively abrogated TS-triggered gastric activation of ERK1/2 and JNK MAPK pathways, AP-1 proteins, and EMT alterations. These results suggest for the first time the protective effects of curcumin in long-term TS exposure-induced gastric MAPK activation and EMT, thus providing new insights into the pathogenesis and chemoprevention of TS-associated gastric cancer. Copyright © 2015 John Wiley & Sons, Ltd.
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