Hepatoprotective Effect of Flavonoid Glycosides from Lespedeza cuneata against Oxidative Stress Induced by tert-Butyl Hyperoxide
Sang Min Kim
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorKyungsu Kang
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorEun Hye Jho
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorYu-Jin Jung
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University, Gangneung, 210-702 Republic of Korea
Search for more papers by this authorChu Won Nho
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorByung-Hun Um
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorCorresponding Author
Cheol-Ho Pan
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Cheol-Ho Pan, Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, 290 Daejeon-dong, Gangneung 210-340, Republic of Korea.
E–mail: [email protected]
Search for more papers by this authorSang Min Kim
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorKyungsu Kang
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorEun Hye Jho
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorYu-Jin Jung
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University, Gangneung, 210-702 Republic of Korea
Search for more papers by this authorChu Won Nho
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorByung-Hun Um
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Search for more papers by this authorCorresponding Author
Cheol-Ho Pan
Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, 210-340 Republic of Korea
Cheol-Ho Pan, Natural Products Research Center, Korea Institute of Science and Technology Gangneung Institute, 290 Daejeon-dong, Gangneung 210-340, Republic of Korea.
E–mail: [email protected]
Search for more papers by this authorAbstract
The aerial parts of Lespedeza cuneata G. Don, perennial legume native to Eastern Asia, have been used therapeutically in traditional Asian medicine to protect the function of liver, kidneys and lungs. However, little is known about the pharmaceutical effect of extracts from this plant. In the present study, the aerial parts of L. cuneata were used to prepare an ethanol extract, which was then tested for hepatoprotective effects against injury by tert-butyl hyperoxide (t-BHP). At a dose of 20 μg/mL, the ethanol extract significantly protected HepG2 cells against the cytotoxicity of t-BHP. Further fractionation of the extract with ethyl acetate allowed the isolation of five flavonoid compounds that were structurally identified by 1H and 13C NMR spectroscopy as isovitexin, hirsutrin, trifolin, avicularin and quercetin. Hirsutrin, avicularin and quercetin (10 μm) showed clear hepatoprotective activity against injury by t-BHP in HepG2 cells, whereas isovitexin and trifolin showed no protective effects. The observed hepatoprotective effect of the investigated compounds showed a high correlation with radical scavenging activity, which followed the structure–activity relationships of the flavonoid aglycones. Copyright © 2011 John Wiley & Sons, Ltd.
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