In vitro Anti-Herpes Simplex Virus Activity of 1,2,4,6-Tetra-O-galloyl-β-d-glucose from Phyllanthus emblica L. (Euphorbiaceae)
Yangfei Xiang
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorYing Pei
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorChang Qu
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorZhicai Lai
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorZhe Ren
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorKe Yang
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorSheng Xiong
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorCorresponding Author
Yingjun Zhang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China
Yifei Wang, Kaio Kitazato and Yingjun Zhang, Biomedicine Research and Development Center, Jinan University, 510632 Guangzhou, Guangdong, PR China.
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorChongren Yang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China
Search for more papers by this authorDong Wang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China
Search for more papers by this authorQing Liu
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China
Search for more papers by this authorCorresponding Author
Kaio Kitazato
Laboratory of Molecular Biology of Infectious Agents, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
Yifei Wang, Kaio Kitazato and Yingjun Zhang, Biomedicine Research and Development Center, Jinan University, 510632 Guangzhou, Guangdong, PR China.
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Yifei Wang
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Yifei Wang, Kaio Kitazato and Yingjun Zhang, Biomedicine Research and Development Center, Jinan University, 510632 Guangzhou, Guangdong, PR China.
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorYangfei Xiang
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorYing Pei
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorChang Qu
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorZhicai Lai
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorZhe Ren
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorKe Yang
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorSheng Xiong
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Search for more papers by this authorCorresponding Author
Yingjun Zhang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China
Yifei Wang, Kaio Kitazato and Yingjun Zhang, Biomedicine Research and Development Center, Jinan University, 510632 Guangzhou, Guangdong, PR China.
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorChongren Yang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China
Search for more papers by this authorDong Wang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China
Search for more papers by this authorQing Liu
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, PR China
Search for more papers by this authorCorresponding Author
Kaio Kitazato
Laboratory of Molecular Biology of Infectious Agents, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
Yifei Wang, Kaio Kitazato and Yingjun Zhang, Biomedicine Research and Development Center, Jinan University, 510632 Guangzhou, Guangdong, PR China.
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Yifei Wang
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, PR China
National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, PR China
Yifei Wang, Kaio Kitazato and Yingjun Zhang, Biomedicine Research and Development Center, Jinan University, 510632 Guangzhou, Guangdong, PR China.
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorAbstract
In this study, 1,2,4,6-tetra-O-galloyl-β-d-glucose (1246TGG), a polyphenolic compound isolated from traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae), was found to inhibit herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infection at different magnitudes of activity in vitro. Further studies revealed that 1246TGG directly inactivated HSV-1 particles, leading to the failure of early infection, including viral attachment and penetration. 1246TGG also suppressed the intracellular growth of HSV-1 within a long period post-infection (from 0 h p.i. to 12 h p.i.), while it might exert an antiviral effect mainly before 3 h p.i. It inhibited HSV-1 E and L gene expressions as well as viral DNA replication but did not affect the RNA synthesis of IE gene in our study. Also, in the presence of 1246TGG, the synthesis of viral protein was reduced. Taken together, it was suggested that 1246TGG might exert anti-HSV activity both by inactivating extracellular viral particles and by inhibiting viral biosynthesis in host cells. These results warrant further studies on the antiviral mechanisms of 1246TGG and suggest that it might be a candidate for HSV therapy. Copyright © 2011 John Wiley & Sons, Ltd.
REFERENCES
- Burns WH, Saral R, Santos GW et al. 1982. Isolation and characterization of resistant herpes simplex virus after acyclovir therapy. Lancet 1: 421–423.
- Cheng HY, Lin TC, Yang CM, Wang KC, Lin LT, Lin CC. 2004. Putranjivain A from Euphorbia jolkini inhibits both virus entry and late stage replication of herpes simplex virus type 2 in vitro. J Antimicrob Chemother 53: 577–583.
- Cheng HY, Lin TC, Yang CM, Shieh DE, Lin CC. 2005. In vitro anti-HSV-2 activity and mechanism of action of proanthocyanidin A-1 from Vaccinium vitis-idaea. J Sci Food Agric 85: 10–15.
- Coen DM, Schaffer PA. 2003. Antiherpesvirus drugs: a promising spectrum of new drugs and drug targets. Nat Rev Drug Discov 2: 278–288.
- Corey L, Adams HG, Brown ZA, Holmes KK. 1983. Genital herpes simplex virus infections: clinical manifestations, course, and complications. Ann Intern Med 98: 958–972.
- Crumpacker CS, Schnipper LE, Marlowe SI, Kowalsky PN, Hershey BJ, Levin MJ. 1982. Resistance to antiviral drugs of herpes simplex virus isolated from a patient treated with acyclovir. N Engl J Med 306: 343–346.
- Isaacs CE, Wen GY, Xu W et al. 2008. Epigallocatechin gallate inactivates clinical isolates of herpes simplex virus. Antimicrob Agents Chemother 52: 962–970.
- Kalinyak JE, Fleagle G, Docherty JJ. 1977. Incidence and distribution of herpes simplex virus types 1 and 2 from genital lesions in college women. J Med Virol 1: 175–181.
- Khan MT, Ather A, Thompson KD, Gambari R. 2005. Extracts and molecules from medicinal plants against herpes simplex viruses. Antivir Res 67: 107–119.
- Kurokawa M, Hozumi T, Basnet P et al. 1998. Purification and characterization of eugeniin as an anti-herpesvirus compound from Geum japonicum and Syzygium aromaticum. J Pharmacol Exp Ther 284: 728–735.
- Kurokawa M, Hozumi T, Tsurita M, Kadota S, Namba T, Shiraki K. 2001. Biological characterization of eugeniin as an anti-herpes simplex virus type 1 compound in vitro and in vivo. J Pharmacol Exp Ther 297: 372–379.
- Leung DT, Sacks SL. 2004. Docosanol: a topical antiviral for herpes labialis. Expert Opin Pharmacother 5: 2567–2571.
- Lin LC, Kuo YC, Chou CJ. 2000. Anti-herpes simplex virus type-1 flavonoids and a new flavanone from the root of Limonium sinense. Planta Med 66: 333–336.
- Lyu SY, Rhim JY, Park WB. 2005. Antiherpetic activities of flavonoids against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in vitro. Arch Pharm Res 28: 1293–1301.
- Nitta K, Shiota H, Nito T, Mimura Y. 1994. Sensitivities to other antiviral drugs and thymidine kinase activity of aciclovir-resistant herpes simplex virus type 1. Nippon Ganka Gakkai Zassh 98: 513–519.
- Russell WC. 1962. A sensitive and precise plaque assay for herpes virus. Nature 195: 1028–1029.
- Sibrack CD, Gutman LT, Wilfert CM et al. 1982. Pathogenicity of acyclovir resistant herpes simplex virus type 1 from an immunodeficient child. J Infect Dis 146: 673–682.
- Song JM, Lee KH, Seong BL. 2005. Antiviral effect of catechins in green tea on influenza virus. Antiviral Res 68: 66–74.
- Su CT, Hsu JT, Hsieh HP et al. 2008. Anti-HSV activity of digitoxin and its possible mechanisms. Antiviral Res 79: 62–70.
- Uozaki M, Yamasaki H, Katsuyama Y, Hiquchi M, Hiquti T, Koyama AH. 2007. Antiviral effect of octyl gallate against DNA and RNA viruses. Antiviral Res 73: 85–91.
- Whitley RJ, Kimberlin DW, Roizman B. 1998. Herpes simplex viruses. Clin Infect Dis 26: 541–553.
- Xia Q, Xiao PG, Wang LW, Kong J. 1997. Ethnopharmacology of Phyllanthus emblica L. Zhongguo Zhongyao Zazhi 22: 515–518.
- Yang CM, Cheng HY, Lin TC, Chiang LC, Lin CC. 2007. The in vitro activity of geraniin and 1,3,4,6-tetra-O-galloyl-β-d-glucose isolated from Phyllanthus urinaria against herpes simplex virus type 1 and type 2 infection. J Ethnopharmacol 110: 555–558.
- Zhang YJ, Abe T, Tanaka T, Yang CR, Kouno I. 2002. Two new acylated flavanone glycosides from the leaves and branches of Phyllanthus emblica. Chem Pharm Bull 50: 841–843.
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