Positive contrast visualization of iron oxide-labeled stem cells using inversion-recovery with ON-resonant water suppression (IRON)
Corresponding Author
Matthias Stuber
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Electrical and Computer Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Medicine, Cardiology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Matthias Stuber is compensated as a consultant by Philips Medical Systems NL, the manufacturer of equipment described in this presentation. The terms of this arrangement have been approved by the Johns Hopkins University in accordance with its conflict of interest policies.
Matthias Stuber, PhD, The Johns Hopkins University School of Medicine, Russell H. Morgan Department of Radiology and Radiological Science; JHOC 4223, 601 North Caroline St., Baltimore, MD, 21287===Search for more papers by this authorWesley D. Gilson
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorMichael Schär
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Philips Medical Systems, Cleveland, Ohio, USA
Search for more papers by this authorDorota A. Kedziorek
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorLawrence V. Hofmann
Interventional Radiology, Stanford University School of Medicine, Stanford, California, USA
Search for more papers by this authorSaurabh Shah
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorEvert-Jan Vonken
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorJeff W.M. Bulte
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorDara L. Kraitchman
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorCorresponding Author
Matthias Stuber
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Electrical and Computer Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Medicine, Cardiology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Matthias Stuber is compensated as a consultant by Philips Medical Systems NL, the manufacturer of equipment described in this presentation. The terms of this arrangement have been approved by the Johns Hopkins University in accordance with its conflict of interest policies.
Matthias Stuber, PhD, The Johns Hopkins University School of Medicine, Russell H. Morgan Department of Radiology and Radiological Science; JHOC 4223, 601 North Caroline St., Baltimore, MD, 21287===Search for more papers by this authorWesley D. Gilson
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorMichael Schär
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Philips Medical Systems, Cleveland, Ohio, USA
Search for more papers by this authorDorota A. Kedziorek
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorLawrence V. Hofmann
Interventional Radiology, Stanford University School of Medicine, Stanford, California, USA
Search for more papers by this authorSaurabh Shah
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorEvert-Jan Vonken
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorJeff W.M. Bulte
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorDara L. Kraitchman
Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Search for more papers by this authorAbstract
In proton magnetic resonance imaging (MRI) metallic substances lead to magnetic field distortions that often result in signal voids in the adjacent anatomic structures. Thus, metallic objects and superparamagnetic iron oxide (SPIO)-labeled cells appear as hypointense artifacts that obscure the underlying anatomy. The ability to illuminate these structures with positive contrast would enhance noninvasive MR tracking of cellular therapeutics. Therefore, an MRI methodology that selectively highlights areas of metallic objects has been developed. Inversion-recovery with ON-resonant water suppression (IRON) employs inversion of the magnetization in conjunction with a spectrally-selective on-resonant saturation prepulse. If imaging is performed after these prepulses, positive signal is obtained from off-resonant protons in close proximity to the metallic objects. The first successful use of IRON to produce positive contrast in areas of metallic spheres and SPIO-labeled stem cells in vitro and in vivo is presented. Magn Reson Med 58:1072–1077, 2007. © 2007 Wiley-Liss, Inc.
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