Bamboo Leaf-Like Micro-Nano Sheets Self-Assembled by Block Copolymers as Wafers for Cells
Corresponding Author
Wen Zhu
Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, 100190 China
Search for more papers by this authorBo Peng
School of Chemistry and Chemical Engineering, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, No. 135, Xingang Xi Road, Guangzhou, 510275 China
Search for more papers by this authorJing Wang
Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, 100190 China
Search for more papers by this authorKe Zhang
Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, 100190 China
Search for more papers by this authorCorresponding Author
Lixin Liu
School of Chemistry and Chemical Engineering, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, No. 135, Xingang Xi Road, Guangzhou, 510275 China
Search for more papers by this authorCorresponding Author
Yongming Chen
School of Chemistry and Chemical Engineering, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, No. 135, Xingang Xi Road, Guangzhou, 510275 China
Search for more papers by this authorCorresponding Author
Wen Zhu
Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, 100190 China
Search for more papers by this authorBo Peng
School of Chemistry and Chemical Engineering, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, No. 135, Xingang Xi Road, Guangzhou, 510275 China
Search for more papers by this authorJing Wang
Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, 100190 China
Search for more papers by this authorKe Zhang
Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, The Chinese Academy of Sciences, Beijing, 100190 China
Search for more papers by this authorCorresponding Author
Lixin Liu
School of Chemistry and Chemical Engineering, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, No. 135, Xingang Xi Road, Guangzhou, 510275 China
Search for more papers by this authorCorresponding Author
Yongming Chen
School of Chemistry and Chemical Engineering, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, No. 135, Xingang Xi Road, Guangzhou, 510275 China
Search for more papers by this authorAbstract
Bamboo leaf shaped poly(ethylene oxide)-b-poly(ϵ-caprolactone) (PEO-b-PCL) sheets were prepared via crystallization-driven self-assembly. By selecting an appropriate mixed solvent, the polymer sheets, with a PCL single-crystal layer sandwiched by two PEO layers, were obtained efficiently. The morphology and structure of the sheets were characterized by microscopes and diffraction techniques. As a non-spherical model particle, endocytosis of the sheets was investigated on RAW 264.7, U937, HUVECs, HeLa, and 293T cells. The polymer sheets, just like wafers for cells, displayed a selective internalization to different cells, which showed a potential application in accurate cell targeting drug delivery and imaging.
Supporting Information
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| Filename | Description |
|---|---|
| mabi201400283-sm-0001-SuppFig-S1.docx3.7 MB |
Figure S1: SEC curves of PEO114-b-PCL95-OH and PEO114-b-PCL95-RhB. Figure S2: Characterization of polymer sheets prepared by PEO114-b-PCL95 (without RhB). (a) TEM image, (b) AFM image, and (c) section analysis of image b. Figure S3: Time-lapse CLSM video clips of RAW264.7 cells at 0, 10, 20, 30, 40 and 50 min after co-culturing with the leaf-like sheets. Figure S4: Time-lapse CLSM video clips of U937 cells (nucleus-blue, membrane-green) at 15, 30, 60, 120, and 180 min after co-culturing with the leaf-like sheets (red). The last picture shows 180 min clip without cell membrane color. Table S1: The conditions and results of polymer sheet formation. Table S2: The physical meanings of the parameters in the formula. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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