Cell Motility on Polyethylene Glycol Block Copolymers Correlates to Fibronectin Surface Adsorption
Peter J. F. Röttgermann
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Peter J. F. Röttgermann and Samira Hertrich contributed equally to this work.Search for more papers by this authorSamira Hertrich
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Peter J. F. Röttgermann and Samira Hertrich contributed equally to this work.Search for more papers by this authorIda Berts
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorMax Albert
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorFelix J. Segerer
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorJean-François Moulin
Helmholtz Zentrum Geesthacht, Institut für Werkstoffforschung, FRM II, Lichtenbergstr. 1, 85747 Garching, Germany
Search for more papers by this authorBert Nickel
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorCorresponding Author
Joachim O. Rädler
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorPeter J. F. Röttgermann
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Peter J. F. Röttgermann and Samira Hertrich contributed equally to this work.Search for more papers by this authorSamira Hertrich
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Peter J. F. Röttgermann and Samira Hertrich contributed equally to this work.Search for more papers by this authorIda Berts
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorMax Albert
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorFelix J. Segerer
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorJean-François Moulin
Helmholtz Zentrum Geesthacht, Institut für Werkstoffforschung, FRM II, Lichtenbergstr. 1, 85747 Garching, Germany
Search for more papers by this authorBert Nickel
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorCorresponding Author
Joachim O. Rädler
Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Search for more papers by this authorAbstract
Adhesion and motility of cells on polyethylene glycol (PEG) engineered surfaces are of fundamental interest for the development of biotechnological devices. Here, the structure of PEG block copolymers physisorbed to surfaces by polyLlysine (PLL) or polypropylene oxide (PPO) is studied. Cell behavior on such surfaces incubated with fibronectin (FN) is analyzed via time-lapse microscopy, the amount and the location of FN is determined via neutron reflectivity. While FN does not adsorb onto PPOPEG, 0.4–0.7 mg m−2 of FN is found in the vicinity of the PLL moiety of PLLPEG. Cells exhibit 21% increased motility on PLLPEG (5 kDa PEG chains) compared to pure FN layers, and 12% decreased motility for PLLPEG (2 kDa PEG chains). These findings suggest that by design of PEGylated surfaces cell migration can be controlled.
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