Rapid Flow Through Immunoassay for CRP Determination Based on Polyethylene Filters Modified with ω-Aminocellulose Carbamate
Thomas Elschner
Center of Excellence for Polysaccharide Research, Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University of Jena, Humboldtstraße 10, D-07743 Jena, Germany
Search for more papers by this authorFriedrich Scholz
Center of Excellence for Polysaccharide Research, Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University of Jena, Humboldtstraße 10, D-07743 Jena, Germany
Senova Gesellschaft für Biowissenschaft und Technik mbH, Industriestraße 8, D-99427 Weimar, Germany
Search for more papers by this authorPeter Miethe
fzmb GmbH, Forschungszentrum für Medizintechnik und Biotechnologie, Geranienweg 7, D-99947 Bad Langensalza, Germany
Search for more papers by this authorCorresponding Author
Thomas Heinze
Center of Excellence for Polysaccharide Research, Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University of Jena, Humboldtstraße 10, D-07743 Jena, Germany
Search for more papers by this authorThomas Elschner
Center of Excellence for Polysaccharide Research, Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University of Jena, Humboldtstraße 10, D-07743 Jena, Germany
Search for more papers by this authorFriedrich Scholz
Center of Excellence for Polysaccharide Research, Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University of Jena, Humboldtstraße 10, D-07743 Jena, Germany
Senova Gesellschaft für Biowissenschaft und Technik mbH, Industriestraße 8, D-99427 Weimar, Germany
Search for more papers by this authorPeter Miethe
fzmb GmbH, Forschungszentrum für Medizintechnik und Biotechnologie, Geranienweg 7, D-99947 Bad Langensalza, Germany
Search for more papers by this authorCorresponding Author
Thomas Heinze
Center of Excellence for Polysaccharide Research, Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University of Jena, Humboldtstraße 10, D-07743 Jena, Germany
Search for more papers by this authorAbstract
A novel method for antibody immobilization is discussed and applied in a column based flow through immunoassay system (ABICAP). It is shown that porous polyethylene material can be modified with different ω-aminocellulose carbamates yielding an amino group containing biocompatible support for antibody immobilization. Anti-h CRP antibodies can be bound covalently to the surface via various homobifunctional cross-linkers. The antibody modified filters are validated for the CRP determination in a sandwich ABICAP system. In a 10 min test procedure based on colloidal dye particles, a limit of detection of 5 ng CRP mL−1 and coefficients of variation of <9.1% are obtained.
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