Volume 128, Issue 1 pp. E33-E40
Laryngology

Adipose-derived mesenchymal stromal cells prevented rat vocal fold scarring

Tsuyoshi Morisaki MD

Tsuyoshi Morisaki MD

Department of Otolaryngology–Head and Neck Surgery, Faculty of Medicine, Tottori University, Tottori, Japan

Search for more papers by this author
Yo Kishimoto MD, PhD

Yo Kishimoto MD, PhD

Department of Otolaryngology–Head and Neck Surgery Graduate School of Medicine, Kyoto University, Kyoto, Japan

Search for more papers by this author
Ichiro Tateya MD, PhD

Ichiro Tateya MD, PhD

Department of Otolaryngology–Head and Neck Surgery Graduate School of Medicine, Kyoto University, Kyoto, Japan

Search for more papers by this author
Yoshitaka Kawai MD

Yoshitaka Kawai MD

Department of Otolaryngology–Head and Neck Surgery Graduate School of Medicine, Kyoto University, Kyoto, Japan

Search for more papers by this author
Ryo Suzuki MD

Ryo Suzuki MD

Department of Otolaryngology–Head and Neck Surgery Graduate School of Medicine, Kyoto University, Kyoto, Japan

Search for more papers by this author
Takuya Tsuji MD

Takuya Tsuji MD

Department of Otolaryngology–Head and Neck Surgery Graduate School of Medicine, Kyoto University, Kyoto, Japan

Search for more papers by this author
Nao Hiwatashi MD, PhD

Nao Hiwatashi MD, PhD

Department of Otolaryngology–Head and Neck Surgery Graduate School of Medicine, Kyoto University, Kyoto, Japan

Department of Otolaryngology–Head and Neck Surgery, NYU Voice Center, New York University School of Medicine, New York, New York, U.S.A.

Search for more papers by this author
Tatsuo Nakamura MD, PhD

Tatsuo Nakamura MD, PhD

Department of Bioartificial Organs, Institute for Frontier Medical Science, Kyoto University, Kyoto, Japan

Search for more papers by this author
Koichi Omori MD, PhD

Koichi Omori MD, PhD

Department of Otolaryngology–Head and Neck Surgery Graduate School of Medicine, Kyoto University, Kyoto, Japan

Search for more papers by this author
Hiroya Kitano MD, PhD

Hiroya Kitano MD, PhD

Department of Otolaryngology–Head and Neck Surgery, Faculty of Medicine, Tottori University, Tottori, Japan

Search for more papers by this author
Hiromi Takeuchi MD, PhD

Hiromi Takeuchi MD, PhD

Department of Otolaryngology–Head and Neck Surgery, Faculty of Medicine, Tottori University, Tottori, Japan

Search for more papers by this author
Shigeru Hirano MD, PhD

Corresponding Author

Shigeru Hirano MD, PhD

Department of Otolaryngology–Head and Neck Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan

Send correspondence to Shigeru Hirano, MD, Department of Otolaryngology–Head and Neck Surgery, Kyoto Prefectural University of Medicine, 465, Kajii-cho, Kamigyo-ku, Kyoto, Japan. E-mail: [email protected]Search for more papers by this author
First published: 20 September 2017
Citations: 22

This work was performed at Kyoto University, Kyoto, Japan.

Presented at the 138th Annual Meeting of the American Laryngological Association, San Diego, California, U.S.A., April 26–28, 2017.

The authors have no other funding, financial relationships, or conflicts of interest to disclose.

Abstract

Objectives/Hypothesis

This study aimed to reveal the effects of adipose-derived mesenchymal stromal cells (ASCs) on prevention of vocal fold scarring by investigating how the immediate ASCs transplantation into the injured rat vocal fold affect the levels of gene transcription and translation.

Study Design

Prospective animal experiments with controls.

Methods

ASCs harvested from green fluorescent protein transgenic rat (ASCs group) or saline (sham group) were injected into the thyroarytenoid muscle of Sprague-Dawley rats immediately after stripping the vocal fold. For histological examinations, larynges were extirpated at 3, 14, and 56 days after the injection. Quantitative real-time polymerase chain reaction (PCR) analyses were performed at 3 and 14 days after the injection.

Results

Transplanted ASCs were detected only in larynges at day 3. At days 14 and 56, histological examination showed significantly higher amounts of hyaluronic acid and lower deposition of collagen in the ASCs group compared to the sham group. Real-time PCR revealed that the ASCs group showed low expression of procollagen (Col)1a1, Col1a3, matrix metalloproteinase (Mmp)1 and Mmp8 in each time points. The ASCs group showed high expression of fibroblast growth factor (Fgf)2 and Hepatocyte growth factor (Hgf) compared to the sham group at day 14.

Conclusions

ASCs increased expressions of Fgf2 and Hgf, and suppressed excessive collagen deposition during vocal fold wound healing. Given the fact that ASCs survived no more than 14 days, ASCs were thought to induce upregulations of growth factors' genes in surrounding cells. These results suggested that ASCs have potential to prevent vocal fold scarring.

Level of Evidence

NA. Laryngoscope, 128:E33–E40, 2018

The full text of this article hosted at iucr.org is unavailable due to technical difficulties.