Volume 96, Issue 11 pp. 3140-3143

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Effects of various cyclodextrins on the stability of freeze-dried lactate dehydrogenase

Junya Iwai,

Junya Iwai

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

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Noriko Ogawa,

Noriko Ogawa

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

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Hiromasa Nagase,

Hiromasa Nagase

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

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Tomohiro Endo,

Tomohiro Endo

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

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Thorsteinn Loftsson,

Thorsteinn Loftsson

Faculty of Pharmacy, University of Iceland, Hofsvallagata 53, IS-107 Reykjavik, Iceland

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Haruhisa Ueda,

Corresponding Author

Haruhisa Ueda

[email protected]

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan. Telephone: +81-3-5498-5766; Fax: +81-3-5498-5159Search for more papers by this author

Junya Iwai,

Junya Iwai

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

Search for more papers by this author

Noriko Ogawa,

Noriko Ogawa

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

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Hiromasa Nagase,

Hiromasa Nagase

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

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Tomohiro Endo,

Tomohiro Endo

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

Search for more papers by this author

Thorsteinn Loftsson,

Thorsteinn Loftsson

Faculty of Pharmacy, University of Iceland, Hofsvallagata 53, IS-107 Reykjavik, Iceland

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Haruhisa Ueda,

Corresponding Author

Haruhisa Ueda

[email protected]

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan

Department of Physical Chemistry, Hoshi University, 4-41, Ebara 2-chome, Shinagawa–ku, Tokyo 142-8501, Japan. Telephone: +81-3-5498-5766; Fax: +81-3-5498-5159Search for more papers by this author

First published: 25 September 2007

https://doi.org/10.1002/jps.20847

Citations: 3

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Abstract

The effects of eight cyclodextrins (CDs) on the stability of freeze-dried lactate dehydrogenase (LDH) were investigated. Five low-molecular weight saccharides were used as a reference. In the samples without saccharides, LDH activity was decreased by freeze-drying. The LDH activities of the samples with 2-hydroxypropylated CDs (HP-CDs) such as 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) showed only a small decrease and were more effective protein stabilizers than low-molecular weight saccharides. HP-β-CD more effectively stabilized LDH than trehalose, at a sugar concentration of less than 1.0 w/w% and furthermore, a close relationship existed between the highly stabilizing effect and the degree of substitution of polar substituents. © 2007 Wiley-Liss, Inc. 96: 3140–3143, 2007

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Volume96, Issue11

November 2007

Pages 3140-3143

Effects of various cyclodextrins on the stability of freeze-dried lactate dehydrogenase

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Effects of various cyclodextrins on the stability of freeze-dried lactate dehydrogenase

Abstract

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