Hydrogen peroxide regulates glucose-regulated protein 78 expression via a cyclooxygenase-2 dependent mechanism
Ying-ying Chen
National Education Base for Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Department of Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Search for more papers by this authorMing-zhi Zheng
Department of Pharmacology, Zhejiang Medical College, Hangzhou 310053, People's Republic of China
Search for more papers by this authorPing-ping Lv
National Education Base for Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Search for more papers by this authorLin Hu
Pharmaceutical Department, 302 Military Hospital of China, Beijing 100039, People's Republic of China
Search for more papers by this authorLin-lin Wang
Center for Stem Cell and Tissue Engineering, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Search for more papers by this authorCorresponding Author
Yue-Liang Shen
Department of Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Department of Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of ChinaSearch for more papers by this authorYing-ying Chen
National Education Base for Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Department of Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Search for more papers by this authorMing-zhi Zheng
Department of Pharmacology, Zhejiang Medical College, Hangzhou 310053, People's Republic of China
Search for more papers by this authorPing-ping Lv
National Education Base for Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Search for more papers by this authorLin Hu
Pharmaceutical Department, 302 Military Hospital of China, Beijing 100039, People's Republic of China
Search for more papers by this authorLin-lin Wang
Center for Stem Cell and Tissue Engineering, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Search for more papers by this authorCorresponding Author
Yue-Liang Shen
Department of Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of China
Department of Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, People's Republic of ChinaSearch for more papers by this authorAbstract
This study was designed to investigate the effect of hydrogen peroxide on the expression of endoplasmic reticulum stress marker glucose-regulated protein 78 (GRP78) in endothelial cells and reveals the possible role of cyclooxygenase in this effect. The porcine endothelial cell line was cultured in 1640 medium. Western blot and immunocytochemistry were used to detect the expression of GRP78. The caspase-12 activity was analyzed with the immune fluorescence method. The results showed that after the endothelial cells were incubated with 250 μM of hydrogen peroxide for 12 h, apoptosis increased, which was antagonized by the cyclooxygenase-2 inhibitor nimesulide or the nonselective cyclooxygenase inhibitor aspirin, but not by the cyclooxygenase-1 inhibitor piroxicam. The expression of GRP78 was induced in endothelial cells after exposure to hydrogen peroxide for 12 h. The overexpression of GRP78 was inhibited by nimesulide and aspirin, but not by piroxicam. There are no significant differences in caspase-12 activity among all groups. The present study provides evidence that hydrogen peroxide induced GRP78 overexpression in endothelial cells by a mechanism involving cyclooxygenase-2-dependent pathway. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:279–285, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20336
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