A Compromised Maxillofacial Wound Healing Model for Characterization of Particulate Bone Grafting: An In Vivo Study in Rabbits
Nourhan Hussein
Katz Department of Oral and Maxillofacial Surgery, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas, USA
Search for more papers by this authorVasudev Vivekanand Nayak
Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA
Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute (BioNIUM), University of Miami, Miami, Florida, USA
Search for more papers by this authorNeeraja Dharmaraj
Katz Department of Oral and Maxillofacial Surgery, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas, USA
Search for more papers by this authorNicholas A. Mirsky
University of Miami Miller School of Medicine, Miami, Florida, USA
Search for more papers by this authorWilliam Norton
University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Search for more papers by this authorLori Ramagli
University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Search for more papers by this authorRamesh Tailor
University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Search for more papers by this authorF. Kurtis Kasper
Department of Orthodontics, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas, USA
Search for more papers by this authorPaulo G. Coelho
Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA
Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute (BioNIUM), University of Miami, Miami, Florida, USA
DeWitt Daughtry Family Department of Surgery, Division of Plastic Surgery, University of Miami Miller School of Medicine, Miami, Florida, USA
Search for more papers by this authorCorresponding Author
Lukasz Witek
Biomaterials and Regenerative Biology Division, NYU College of Dentistry, New York, New York, USA
Department of Biomedical Engineering, NYU Tandon School of Engineering, Brooklyn, New York, USA
Hansjörg Wyss Department of Plastic Surgery, NYU Grossman School of Medicine, New York, New York, USA
Correspondence:
Lukasz Witek ([email protected])
Search for more papers by this authorSimon Young
Katz Department of Oral and Maxillofacial Surgery, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas, USA
Search for more papers by this authorNourhan Hussein
Katz Department of Oral and Maxillofacial Surgery, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas, USA
Search for more papers by this authorVasudev Vivekanand Nayak
Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA
Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute (BioNIUM), University of Miami, Miami, Florida, USA
Search for more papers by this authorNeeraja Dharmaraj
Katz Department of Oral and Maxillofacial Surgery, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas, USA
Search for more papers by this authorNicholas A. Mirsky
University of Miami Miller School of Medicine, Miami, Florida, USA
Search for more papers by this authorWilliam Norton
University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Search for more papers by this authorLori Ramagli
University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Search for more papers by this authorRamesh Tailor
University of Texas MD Anderson Cancer Center, Houston, Texas, USA
Search for more papers by this authorF. Kurtis Kasper
Department of Orthodontics, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas, USA
Search for more papers by this authorPaulo G. Coelho
Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA
Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute (BioNIUM), University of Miami, Miami, Florida, USA
DeWitt Daughtry Family Department of Surgery, Division of Plastic Surgery, University of Miami Miller School of Medicine, Miami, Florida, USA
Search for more papers by this authorCorresponding Author
Lukasz Witek
Biomaterials and Regenerative Biology Division, NYU College of Dentistry, New York, New York, USA
Department of Biomedical Engineering, NYU Tandon School of Engineering, Brooklyn, New York, USA
Hansjörg Wyss Department of Plastic Surgery, NYU Grossman School of Medicine, New York, New York, USA
Correspondence:
Lukasz Witek ([email protected])
Search for more papers by this authorSimon Young
Katz Department of Oral and Maxillofacial Surgery, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas, USA
Search for more papers by this authorFunding: This work was supported by Osteo Science Foundation (Peter Geistlich Research Award).
ABSTRACT
Preclinical testing of tissue engineering modalities are commonly performed in a healthy wound bed. These conditions do not represent clinically relevant compromised oral wound environments due to radiation treatments seen clinically. This study aimed to characterize the bone regeneration outcomes in critical-sized mandibular defects using particulate grafting in an irradiated preclinical model of compromised wound healing. Sixteen New Zealand white rabbits were divided into two groups (n = 8/group), namely (i) irradiated (experimental) and (ii) non-irradiated (control). The rabbits in the experimental group received a total of 36 Gy radiation, followed by surgical intervention to create critical-sized (10 mm), full-thickness mandibular defects. The control group was subjected to the same surgical intervention. All defects were filled with bovine bone grafting material (Bio-Oss, Geistlich, Princeton, NJ, USA) and allowed to heal for 8 weeks. At the study endpoint, rabbits were euthanized, and their mandibles were harvested for micro-computed tomographic, histological, and histomorphometric processing and analysis. Qualitative histological analysis revealed increased levels of bone formation and bridging in the control group relative to the experimental group. This was accompanied by increased levels of soft tissue presence in the experimental group. Volumetric reconstruction showed a significantly higher degree of bone in the control group (27.59% ± 2.71), relative to the experimental group (22.02% ± 2.71) (p = 0.001). The irradiated rabbit model exhibited decreased bone regeneration capacity relative to the healthy subjects, highlighting its suitability as a robust compromised wound healing environment for further preclinical testing involving growth factors or customized, high-fidelity 3D printed tissue engineering scaffolds.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability Statement
Data from this study will be made available upon reasonable request to the corresponding author.
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