Journal of Biomedical Materials Research Part B: Applied Biomaterials

Original Research Report

The optimization of sintering treatment on bovine-derived bone grafts for bone regeneration: in vitro and in vivo evaluation

An-Tian Xu

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Both authors contributed equally to this work.Search for more papers by this author

Wen-Ting Qi,

Wen-Ting Qi

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Both authors contributed equally to this work.Search for more papers by this author

Meng-Na Lin,

Meng-Na Lin

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

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Yu-Hao Zhu,

Yu-Hao Zhu

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

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Fu-Ming He,

Corresponding Author

Fu-Ming He

[email protected]

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Correspondence to: F.-M. He; e-mail: [email protected]Search for more papers by this author

An-Tian Xu,

An-Tian Xu

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Both authors contributed equally to this work.Search for more papers by this author

Wen-Ting Qi,

Wen-Ting Qi

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Both authors contributed equally to this work.Search for more papers by this author

Meng-Na Lin,

Meng-Na Lin

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Search for more papers by this author

Yu-Hao Zhu,

Yu-Hao Zhu

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Search for more papers by this author

Fu-Ming He,

Corresponding Author

Fu-Ming He

[email protected]

Department of Prothodontics, The Affiliated Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Correspondence to: F.-M. He; e-mail: [email protected]Search for more papers by this author

First published: 23 April 2019

https://doi.org/10.1002/jbm.b.34387

Citations: 15

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Abstract

Modifications of sintering temperature and treatment time of bovine-derived bone grafts affect their physicochemical properties and further influence biological activity. Three different temperature sintered bovine-derived bone grafts: group I (300 °C 3 h), group II (300 °C 3 h plus 530 °C 6 h), and group III (300 °C 3 h plus 1000 °C 2 h) and Bio-Oss® were characterized and then compared in vitro for their effects on bone marrow stromal cells (BMSCs) migration, proliferation, and differentiation as estimated by cell migration assay, Alkaline phosphatase (ALP) activity assay, and Alizarin red staining. Further, the four bone grafts were implanted into the calvarial defects of rabbits to evaluate bone regeneration and graft degradation. The four deproteinized bovine-derived bone grafts displayed different surface topography. Group II displayed the highest potential of attracting cells. Both groups I and II markedly promote BMSCs differentiation. After 6 and 12 weeks, defects grafted with groups I and II displayed a significant higher bone fraction than defects grafted with group III and Bio-Oss®. Bone graft remnants remained in all four groups. Taken together, sintering at 300 °C for 3 h and sintering at 300 °C for 3 h with an addition of 530 °C for 6 h of bovine-dervied bone grafts displayed potential use in bone regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:272–281, 2020.

CONFLICT OF INTEREST

The authors declare no conflict of interest.

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Volume108, Issue1

January 2020

Pages 272-281

The optimization of sintering treatment on bovine-derived bone grafts for bone regeneration: in vitro and in vivo evaluation

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The optimization of sintering treatment on bovine-derived bone grafts for bone regeneration: in vitro and in vivo evaluation

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