Review Article
3D printed polymer–mineral composite biomaterials for bone tissue engineering: Fabrication and characterization
Joanna Babilotte,
Vera Guduric,
Damien Le Nihouannen,
Adrien Naveau,
Jean-Christophe Fricain,
Sylvain Catros,
Corresponding Author
Joanna Babilotte
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Correspondence to: J. Babbilotte; e-mail: [email protected]Search for more papers by this authorVera Guduric
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Search for more papers by this authorDamien Le Nihouannen
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Search for more papers by this authorAdrien Naveau
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Faculty of Dentistry, University of Bordeaux, Bordeaux, France
Search for more papers by this authorJean-Christophe Fricain
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Faculty of Dentistry, University of Bordeaux, Bordeaux, France
Search for more papers by this authorSylvain Catros
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Faculty of Dentistry, University of Bordeaux, Bordeaux, France
Search for more papers by this authorJoanna Babilotte,
Vera Guduric,
Damien Le Nihouannen,
Adrien Naveau,
Jean-Christophe Fricain,
Sylvain Catros,
Corresponding Author
Joanna Babilotte
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Correspondence to: J. Babbilotte; e-mail: [email protected]Search for more papers by this authorVera Guduric
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Search for more papers by this authorDamien Le Nihouannen
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Search for more papers by this authorAdrien Naveau
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Faculty of Dentistry, University of Bordeaux, Bordeaux, France
Search for more papers by this authorJean-Christophe Fricain
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Faculty of Dentistry, University of Bordeaux, Bordeaux, France
Search for more papers by this authorSylvain Catros
Tissue Bioengineering, University of Bordeaux, Bordeaux, France
Faculty of Dentistry, University of Bordeaux, Bordeaux, France
Search for more papers by this authorAbstract
Applications in additive manufacturing technologies for bone tissue engineering applications requires the development of new biomaterials formulations. Different three-dimensional (3D) printing technologies can be used and polymers are commonly employed to fabricate 3D printed bone scaffolds. However, these materials used alone do not possess an effective osteopromotive potential for bone regeneration. A growing number of studies report the combination of polymers with minerals in order to improve their bioactivity. This review exposes the state-of-the-art of existing 3D printed composite biomaterials combining polymers and minerals for bone tissue engineering. Characterization techniques to assess scaffold properties are also discussed. Several parameters must be considered to fabricate a 3D printed material for bone repair (3D printing method, type of polymer/mineral combination and ratio) because all of them affect final properties of the material. Each polymer and mineral has its own advantages and drawbacks and numerous composites are described in the literature. Each component of these composite materials brings specific properties and their combination can improve the biological integration of the 3D printed scaffold. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2579–2595, 2019.
REFERENCES
- 1Thavornyutikarn B, Chantarapanich N, Sitthiseripratip K, Thouas GA, Chen Q. Bone tissue engineering scaffolding: Computer-aided scaffolding techniques. Prog Biomater 2014; 3(2–4): 61–102.
- 2Bajaj P, Schweller RM, Khademhosseini A, West JL, Bashir R. 3D biofabrication strategies for tissue engineering and regenerative medicine. Annu Rev Biomed Eng 2014; 16: 247–276.
- 3Melchels FPW, Feijen J, Grijpma DW. A review on stereolithography and its applications in biomedical engineering. Biomaterials 2010; 31(24): 6121–6130.
- 4Kim BS, Jang J, Chae S, Gao G, Kong J-S, Ahn M, Cho D-W. Three-dimensional bioprinting of cell-laden constructs with polycaprolactone protective layers for using various thermoplastic polymers. Biofabrication 2016; 8(3):035013.
- 5Ma PX. Scaffolds for tissue fabrication. Mater Today 2004; 7(5): 30–40.
- 6Shrivats AR, McDermott MC, Hollinger JO. Bone tissue engineering: State of the union. Drug Discov Today 2014; 19(6): 781–786.
- 7Dorozhkin SV. Bioceramics of calcium orthophosphates. Biomaterials 2010; 31(7): 1465–1485.
- 8Senatov FS, Niaza KV, Zadorozhnyy MY, Maksimkin AV, Kaloshkin SD, Estrin YZ. Mechanical properties and shape memory effect of 3D-printed PLA-based porous scaffolds. J Mech Behav Biomed Mater 2016; 57: 139–148.
- 9Xu N, Ye X, Wei D, Zhong J, Chen Y, Xu G, He D. 3D artificial bones for bone repair prepared by computed tomography-guided fused deposition modeling for bone repair. ACS Appl Mater Interfaces 2014; 6(17): 14952–14963.
- 10Damadzadeh B, Jabari H, Skrifvars M, Airola K, Moritz N, Vallittu PK. Effect of ceramic filler content on the mechanical and thermal behaviour of poly-L-lactic acid and poly-L-lactic-co-glycolic acid composites for medical applications. J Mater Sci Mater Med 2010; 21(9): 2523–2531.
- 11Simpson RL, Nazhat SN, Blaker JJ, Bismarck A, Hill R, Boccaccini AR, Hansen UN, Amis AA. A comparative study of the effects of different bioactive fillers in PLGA matrix composites and their suitability as bone substitute materials: A thermo-mechanical and in vitro investigation. J Mech Behav Biomed Mater 2015; 50: 277–289.
- 12Roh H-S, Jung S-C, Kook M-S, Kim B-H. In vitro study of 3D PLGA/n-HAp/β-TCP composite scaffolds with etched oxygen plasma surface modification in bone tissue engineering. Appl Surface Sci 2016; 388: 321–330.
- 13Schantz J-T, Brandwood A, Hutmacher DW, Khor HL, Bittner K. Osteogenic differentiation of mesenchymal progenitor cells in computer designed fibrin-polymer-ceramic scaffolds manufactured by fused deposition modeling. J Mater Sci Mater Med 2005; 16(9): 807–819.
- 14Nyberg E, Rindone A, Dorafshar A, Grayson WL. Comparison of 3D-printed poly-ε-caprolactone scaffolds functionalized with tricalcium phosphate, hydroxyapatite, bio-oss, or decellularized bone matrix. Tissue Eng A 2016; 23: 503–514.
- 15Waltman L, van Eck NJ, Noyons ECM. A unified approach to mapping and clustering of bibliometric networks. J Informet 2010; 4(4): 629–635.
- 16Huang J, Xiong J, Liu J, Zhu W, Chen J, Duan L, Zhang J, Wang D. Evaluation of the novel three-dimensional porous poly (L-lactic acid)/nano-hydroxyapatite composite scaffold. Biomed Mater Eng 2015; 26(Suppl 1): S197–S205.
- 17Ma R, Lai Y, Li L, Tan H, Wang J, Li Y, Tang T, Qin L. Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous composite scaffolds–an in vitro efficacy study. Sci Rep 2015; 5: 13775.
- 18Wang J, Wu D, Zhang Z, Li J, Shen Y, Wang Z, Li Y, Zhang Z-Y, Sun J. Biomimetically ornamented rapid prototyping fabrication of an apatite–collagen–Polycaprolactone composite construct with Nano–micro–macro hierarchical structure for large bone defect treatment. ACS Appl Mater Interfaces 2015; 7(47): 26244–26256.
- 19Kim J, McBride S, Tellis B, Alvarez-Urena P, Song Y-H, Dean DD, Sylvia VL, Elgendy H, Ong J, Hollinger JO. Rapid-prototyped PLGA/β-TCP/hydroxyapatite nanocomposite scaffolds in a rabbit femoral defect model. Biofabrication 2012; 4(2):025003.
- 20Serra T, Planell JA, Navarro M. High-resolution PLA-based composite scaffolds via 3-D printing technology. Acta Biomater 2013; 9(3): 5521–5530.
- 21Serra T, Ortiz-Hernandez M, Engel E, Planell JA, Navarro M. Relevance of PEG in PLA-based blends for tissue engineering 3D-printed scaffolds. Mater Sci Eng C 2014; 38: 55–62.
- 22Hung BP, Naved BA, Nyberg EL, Dias M, Holmes CA, Elisseeff JH, Dorafshar AH, Grayson WL. Three-dimensional printing of bone extracellular matrix for craniofacial regeneration. ACS Biomater Sci Eng 2016; 2(10): 1806–1816.
- 23Kutikov AB, Gurijala A, Song J. Rapid prototyping amphiphilic polymer/hydroxyapatite composite scaffolds with hydration-induced self-fixation behavior. Tissue Eng C Methods 2014; 21(3): 229–241.
- 24Liao H-T, Lee M-Y, Wen-Wei T, Wang H-C, Lu W-C. Osteogenesis of adipose-derived stem cells on polycaprolactone–β-tricalcium phosphate scaffold fabricated via selective laser sintering and surface coating with collagen type I. J Tissue Eng Regen Med 2013; 10(10): E337–E353.
- 25Duan B, Wang M. Customized ca-P/PHBV nanocomposite scaffolds for bone tissue engineering: Design, fabrication, surface modification and sustained release of growth factor. J R Soc Interface 2010; 7(Suppl 5): S615–S629.
- 26Chua CK, Leong KF, Tan KH, Wiria FE, Cheah CM. Development of tissue scaffolds using selective laser sintering of polyvinyl alcohol/hydroxyapatite biocomposite for craniofacial and joint defects. J Mater Sci Mater Med 2004; 15(10): 1113–1121.
- 27Dadsetan M, Guda T, Runge MB, Mijares D, LeGeros RZ, LeGeros JP, Silliman DT, Lu L, Wenke JC, Brown Baer PR, Yaszemski MJ. Effect of calcium phosphate coating and rhBMP-2 on bone regeneration in rabbit calvaria using poly(propylene fumarate) scaffolds. Acta Biomater 2015; 18: 9–20.
- 28Lam CXF, Savalani MM, Teoh S-H, Hutmacher DW. Dynamics of in vitro polymer degradation of polycaprolactone-based scaffolds: Accelerated versus simulated physiological conditions. Biomed Mater 2008; 3(3):034108.
- 29Duan B, Wang M, Zhou WY, Cheung WL, Li ZY, Lu WW. Three-dimensional nanocomposite scaffolds fabricated via selective laser sintering for bone tissue engineering. Acta Biomater 2010; 6(12): 4495–4505.
- 30Duan B, Cheung WL, Wang M. Optimized fabrication of Ca–P/PHBV nanocomposite scaffolds via selective laser sintering for bone tissue engineering. Biofabrication 2011; 3(1):015001.
- 31Idaszek J, Bruinink A, Święszkowski W. Ternary composite scaffolds with tailorable degradation rate and highly improved colonization by human bone marrow stromal cells. J Biomed Mater Res A 2015; 103(7): 2394–2404.
- 32Jensen J, Kraft DCE, Lysdahl H, Foldager CB, Chen M, Kristiansen AA, Rölfing JHD, Bünger CE. Functionalization of Polycaprolactone scaffolds with hyaluronic acid and β-TCP facilitates migration and osteogenic differentiation of human dental pulp stem cells in vitro. Tissue Eng A 2015; 21(3–4): 729–739.
- 33Kalita SJ, Bose S, Hosick HL, Bandyopadhyay A. Development of controlled porosity polymer-ceramic composite scaffolds via fused deposition modeling. Mater Sci Eng C 2003; 23(5): 611–620.
- 34Korpela J, Kokkari A, Korhonen H, Malin M, Närhi T, Seppälä J. Biodegradable and bioactive porous scaffold structures prepared using fused deposition modeling. J Biomed Mater Res B Appl Biomater 2013; 101B(4): 610–619.
- 35Lai Y, Cao H, Wang X, Chen S, Zhang M, Wang N, Yao Z, Dai Y, Xie X, Zhang P, Yao X, Qin L. Porous composite scaffold incorporating osteogenic phytomolecule icariin for promoting skeletal regeneration in challenging osteonecrotic bone in rabbits. Biomaterials 2018; 153: 1–13.
- 36Kawai T, Shanjani Y, Fazeli S, Behn AW, Okuzu Y, Goodman SB, Yang YP. Customized, degradable, functionally graded scaffold for potential treatment of early stage osteonecrosis of the femoral head. J Orthop Res 2017; 36(3): 1002–1011.
- 37Lee JW, Ahn G, Kim DS, Cho D-W. Development of nano- and microscale composite 3D scaffolds using PPF/DEF-HA and micro-stereolithography. Microelectron Eng 2009; 86(4): 1465–1467.
- 38Liao H-T, Chen Y-Y, Lai Y-T, Hsieh M-F, Jiang C-P. The osteogenesis of bone marrow stem cells on mPEG-PCL-mPEG/hydroxyapatite composite scaffold via solid freeform fabrication. BioMed Res Int 2014; 2014: 1–13.
- 39Pang L, Hao W, Jiang M, Huang J, Yan Y, Hu Y. Bony defect repair in rabbit using hybrid rapid prototyping polylactic-co-glycolic acid/β-tricalciumphosphate collagen I/apatite scaffold and bone marrow mesenchymal stem cells. Indian J Orthopaed 2013; 47(4): 388–394.
- 40Rai B, Lin JL, Lim ZXH, Guldberg RE, Hutmacher DW, Cool SM. Differences between in vitro viability and differentiation and in vivo bone-forming efficacy of human mesenchymal stem cells cultured on PCL–TCP scaffolds. Biomaterials 2010; 31(31): 7960–7970.
- 41Yu D, Li Q, Mu X, Chang T, Xiong Z. Bone regeneration of critical calvarial defect in goat model by PLGA/TCP/rhBMP-2 scaffolds prepared by low-temperature rapid-prototyping technology. Int J Oral Maxillofac Surg 2008; 37(10): 929–934.
- 42Yeo A, Wong WJ, Teoh S-H. Surface modification of PCL-TCP scaffolds in rabbit calvaria defects: Evaluation of scaffold degradation profile, biomechanical properties and bone healing patterns. J Biomed Mater Res A 2010; 93A(4): 1358–1367.
- 43Chen S-H, Wang X-L, Xie X-H, Zheng L-Z, Yao D, Wang D-P, Leng Y, Zhang G, Qin L. Comparative study of osteogenic potential of a composite scaffold incorporating either endogenous bone morphogenetic protein-2 or exogenous phytomolecule icaritin: An in vitro efficacy study. Acta Biomater 2012; 8(8): 3128–3137.
- 44Xia Y, Zhou P, Cheng X, Xie Y, Liang C, Li C, Xu S. Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications. Int J Nanomed 2013; 8: 4197–4213.
- 45Reichert JC, Cipitria A, Epari DR, Saifzadeh S, Krishnakanth P, Berner A, Woodruff MA, Schell H, Mehta M, Schuetz MA, Duda GN, Hutmacher DW. A tissue engineering solution for segmental defect regeneration in load-bearing long bones. Sci Transl Med 2012; 4(141): 141ra93–141ra93.
- 46Xuan Y, Tang H, Wu B, Ding X, Lu Z, Li W, Xu Z. A specific groove design for individualized healing in a canine partial sternal defect model by a polycaprolactone/hydroxyapatite scaffold coated with bone marrow stromal cells. J Biomed Mater Res A 2014; 102(10): 3401–3408.
- 47Yeo A, Wong WJ, Khoo HH, Teoh SH. Surface modification of PCL-TCP scaffolds improve interfacial mechanical interlock and enhance early bone formation: An in vitro and in vivo characterization. J Biomed Mater Res A 2010; 92A(1): 311–321.
- 48Ding C, Qiao Z, Jiang W, Li H, Wei J, Zhou G, Dai K. Regeneration of a goat femoral head using a tissue-specific, biphasic scaffold fabricated with CAD/CAM technology. Biomaterials 2013; 34(28): 6706–6716.
- 49Zhang H, Mao X, Du Z, Jiang W, Han X, Zhao D, Han D, Li Q. Three dimensional printed macroporous polylactic acid/hydroxyapatite composite scaffolds for promoting bone formation in a critical-size rat calvarial defect model. Sci Technol Adv Mater 2016; 17(1): 136–148.
- 50Liu L, Xiong Z, Yan Y, Hu Y, Zhang R, Wang S. Porous morphology, porosity, mechanical properties of poly(α-hydroxy acid)–tricalcium phosphate composite scaffolds fabricated by low-temperature deposition. J Biomed Mater Res A 2007; 82A(3): 618–629.
- 51Zheng P, Yao Q, Mao F, Liu N, Xu Y, Wei B, Wang L. Adhesion, proliferation and osteogenic differentiation of mesenchymal stem cells in 3D printed poly-ε-caprolactone/hydroxyapatite scaffolds combined with bone marrow clots. Mol Med Rep 2017; 16(4): 5078–5084.
- 52Yeo A, Sju E, Rai B, Teoh SH. Customizing the degradation and load-bearing profile of 3D polycaprolactone-tricalcium phosphate scaffolds under enzymatic and hydrolytic conditions. J Biomed Mater Res B Appl Biomater 2008; 87B(2): 562–569.
- 53Shor L, Güçeri S, Wen X, Gandhi M, Sun W. Fabrication of three-dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro. Biomaterials 2007; 28(35): 5291–5297.
- 54Zhang H, Mao X, Zhao D, Jiang W, Du Z, Li Q, Jiang C, Han D. Three dimensional printed polylactic acid-hydroxyapatite composite scaffolds for prefabricating vascularized tissue engineered bone: An in vivo bioreactor model. Sci Rep 2017; 7(1): 15255.
- 55Guillaume O, Geven MA, Sprecher CM, Stadelmann VA, Grijpma DW, Tang TT, Qin L, Lai Y, Alini M, de Bruijn JD, Yuan H, Richards RG, Eglin D. Surface-enrichment with hydroxyapatite nanoparticles in stereolithography-fabricated composite polymer scaffolds promotes bone repair. Acta Biomater 2017; 54: 386–398.
- 56Li X, Wang Y, Wang Z, Qi Y, Li L, Zhang P, Chen X, Huang Y. Composite PLA/PEG/nHA/dexamethasone scaffold prepared by 3D printing for bone regeneration. Macromol Biosci 2018; 18(6): 1800068.
- 57Song Y, Lin K, He S, Wang C, Zhang S, Li D, Wang J, Cao T, Bi L, Pei G. Nano-biphasic calcium phosphate/polyvinyl alcohol composites with enhanced bioactivity for bone repair via low-temperature three-dimensional printing and loading with platelet-rich fibrin. Int J Nanomed 2018; 13: 505–523.
- 58Huang B, Caetano G, Vyas C, Blaker JJ, Diver C, Bártolo P. Polymer-ceramic composite scaffolds: The effect of hydroxyapatite and β-tri-calcium phosphate. Materials 2018; 11(1): 1–13.
- 59Kim J-Y, Ahn G, Kim C, Lee J-S, Lee I-G, An S-H, Yun W-S, Kim S-Y, Shim J-H. Synergistic effects of Beta tri-calcium phosphate and porcine-derived Decellularized bone extracellular matrix in 3D-printed Polycaprolactone scaffold on bone regeneration. Macromol Biosci 2018; 18(6): 1800025.
- 60Konopnicki S, Sharaf B, Resnick C, Patenaude A, Pogal-Sussman T, Hwang K-G, Abukawa H, Troulis MJ. Tissue-engineered bone with 3-dimensionally printed β-tricalcium phosphate and polycaprolactone scaffolds and early implantation: An in vivo pilot study in a porcine mandible model. J Oral Maxillofac Surg 2015; 73(5): 1016.e1–1016.e11.
- 61Yeo A, Cheok C, Teoh SH, Zhang ZY, Buser D, Bosshardt DD. Lateral ridge augmentation using a PCL-TCP scaffold in a clinically relevant but challenging micropig model. Clin Oral Implants Res 2012; 23(12): 1322–1332.
- 62Arafat MT, Lam CXF, Ekaputra AK, Wong SY, Li X, Gibson I. Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering. Acta Biomater 2011; 7(2): 809–820.
- 63Simpson RL, Wiria FE, Amis AA, Chua CK, Leong KF, Hansen UN, Chandrasekaran M, Lee MW. Development of a 95/5 poly(L-lactide-co-glycolide)/hydroxylapatite and β-tricalcium phosphate scaffold as bone replacement material via selective laser sintering. J Biomed Mater Res B Appl Biomater 2008; 84B(1): 17–25.
- 64Nandakumar A, Barradas A, de Boer J, Moroni L, van Blitterswijk C, Habibovic P. Combining technologies to create bioactive hybrid scaffolds for bone tissue engineering. Biomatter 2013; 3(2): e23705-1–e23705-13.
10.4161/biom.23705 Google Scholar
- 65Bruyas A, Lou F, Stahl AM, Gardner M, Maloney W, Goodman S, Yang YP. Systematic characterization of 3D-printed PCL/β-TCP scaffolds for biomedical devices and bone tissue engineering: Influence of composition and porosity. J Mater Res 2018; 33: 1–12.
- 66Park SH, Park SA, Kang YG, Shin JW, Park YS, Gu SR, Wu YR, Wei J, Shin J-W. PCL/β-TCP composite scaffolds exhibit positive osteogenic differentiation with mechanical stimulation. Tissue Eng Regenerat Med 2017; 14(4): 349–358.
- 67Lam CXF, Olkowski R, Swieszkowski W, Tan KC, Gibson I, Hutmacher DW. Mechanical and in vitro evaluations of composite PLDLLA/TCP scaffolds for bone engineering. Virtual Phys Prototyp 2008; 3(4): 193–197.
10.1080/17452750802551298 Google Scholar
- 68Ma X, Hu YY, Wu XM, Liu J, Xiong Z, Yan YN, Lv R, Wang J. Fabrication of a RP-based biomimetic grafting material for bone tissue engineering. Mater Sci Forum 2009; 627: 553–558.
10.4028/www.scientific.net/MSF.626-627.553 Google Scholar
- 69Xiong Z, Yan Y, Wang S, Zhang R, Zhang C. Fabrication of porous scaffolds for bone tissue engineering via low-temperature deposition. Scr Mater 2002; 46(11): 771–776.
- 70Pang L, Hu Y, Yan Y, Liu L, Xiong Z, Wei Y, Bai J. Surface modification of PLGA/β-TCP scaffold for bone tissue engineering: Hybridization with collagen and apatite. Surf Coat Technol 2007; 201(24): 9549–9557.
- 71Park SA, Lee SH, Kim WD. Fabrication of porous polycaprolactone/hydroxyapatite (PCL/HA) blend scaffolds using a 3D plotting system for bone tissue engineering. Bioprocess Biosyst Eng 2011; 34(4): 505–513.
- 72Zein I, Hutmacher DW, Tan KC, Teoh SH. Fused deposition modeling of novel scaffold architectures for tissue engineering applications. Biomaterials 2002; 23(4): 1169–1185.
- 73Hutmacher DW, Schantz T, Zein I, Ng KW, Teoh SH, Tan KC. Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling. J Biomed Mater Res 2001; 55(2): 203–216.
- 74Temple JP, Hutton DL, Hung BP, Huri PY, Cook CA, Kondragunta R, Jia X, Grayson WL. Engineering anatomically shaped vascularized bone grafts with hASCs and 3D-printed PCL scaffolds. J Biomed Mater Res A 2014; 102(12): 4317–4325.
- 75Costa PF, Puga AM, Díaz-Gomez L, Concheiro A, Busch DH, Alvarez-Lorenzo C. Additive manufacturing of scaffolds with dexamethasone controlled release for enhanced bone regeneration. Int J Pharm 2015; 496(2): 541–550.
- 76Liu W, Wang D, Huang J, Wei Y, Xiong J, Zhu W, Duan L, Chen J, Sun R, Wang D. Low-temperature deposition manufacturing: A novel and promising rapid prototyping technology for the fabrication of tissue-engineered scaffold. Mater Sci Eng C 2017; 70: 976–982.
- 77Bajaj P, Chan V, Jeong JH, Zorlutuna P, Kong H, Bashir R. 3-D biofabrication using stereolithography for biology and medicine. In: 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society. 2012. p. 6805–6808.
- 78Roseti L, Parisi V, Petretta M, Cavallo C, Desando G, Bartolotti I, Grigolo B. Scaffolds for bone tissue engineering: State of the art and new perspectives. Mater Sci Eng C 2017; 78: 1246–1262.
- 79Hinczewski C, Corbel S, Chartier T. Ceramic suspensions suitable for stereolithography. J Eur Ceram Soc 1998; 18(6): 583–590.
- 80Corden TJ, Jones IA, Rudd CD, Christian P, Downes S, McDougall KE. Physical and biocompatibility properties of poly-ε-caprolactone produced using in situ polymerisation: A novel manufacturing technique for long-fibre composite materials. Biomaterials 2000; 21(7): 713–724.
- 81Rasal RM, Janorkar AV, Hirt DE. Poly(lactic acid) modifications. Prog Polym Sci 2010; 35(3): 338–356.
- 82Santos CFL, Silva AP, Lopes L, Pires I, Correia IJ. Design and production of sintered β-tricalcium phosphate 3D scaffolds for bone tissue regeneration. Mater Sci Eng C 2012; 32(5): 1293–1298.
- 83Giannoudis PV, Dinopoulos H, Tsiridis E. Bone substitutes: An update. Injury 2005; 36(Suppl 3): S20–S27.
- 84Naghieh S, Karamooz Ravari MR, Badrossamay M, Foroozmehr E, Kadkhodaei M. Numerical investigation of the mechanical properties of the additive manufactured bone scaffolds fabricated by FDM: The effect of layer penetration and post-heating. J Mech Behav Biomed Mater 2016; 59: 241–250.
- 85Rasperini G, Pilipchuk SP, Flanagan CL, Park CH, Pagni G, Hollister SJ, Giannobile WV. 3D-printed bioresorbable scaffold for periodontal repair. J Dental Res 2015; 94(9_suppl): 153S–157S.
