Original Research Report
In vitro evaluation of poly (lactic-co-glycolic acid)/polyisoprene fibers for soft tissue engineering
Douglas R. Marques,
Luís A. L. dos Santos,
Marie A. O'Brien,
Sarah H. Cartmell,
Julie E. Gough,
Corresponding Author
Douglas R. Marques
Universidade Federal do Rio Grande do Sul, School of Engineering, Porto Alegre, Brazil
University of Manchester, School of Materials, Manchester, United Kingdom
Correspondence to: D.R. Marques; e-mail: [email protected]Search for more papers by this authorLuís A. L. dos Santos
Universidade Federal do Rio Grande do Sul, School of Engineering, Porto Alegre, Brazil
Search for more papers by this authorMarie A. O'Brien
University of Manchester, School of Materials, Manchester, United Kingdom
Search for more papers by this authorSarah H. Cartmell
University of Manchester, School of Materials, Manchester, United Kingdom
Search for more papers by this authorJulie E. Gough
University of Manchester, School of Materials, Manchester, United Kingdom
Search for more papers by this authorDouglas R. Marques,
Luís A. L. dos Santos,
Marie A. O'Brien,
Sarah H. Cartmell,
Julie E. Gough,
Corresponding Author
Douglas R. Marques
Universidade Federal do Rio Grande do Sul, School of Engineering, Porto Alegre, Brazil
University of Manchester, School of Materials, Manchester, United Kingdom
Correspondence to: D.R. Marques; e-mail: [email protected]Search for more papers by this authorLuís A. L. dos Santos
Universidade Federal do Rio Grande do Sul, School of Engineering, Porto Alegre, Brazil
Search for more papers by this authorMarie A. O'Brien
University of Manchester, School of Materials, Manchester, United Kingdom
Search for more papers by this authorSarah H. Cartmell
University of Manchester, School of Materials, Manchester, United Kingdom
Search for more papers by this authorJulie E. Gough
University of Manchester, School of Materials, Manchester, United Kingdom
Search for more papers by this authorAbstract
The polymeric blend of poly (lactic-co-glycolic acid) (PLGA) and polyisoprene (PI) has recently been explored for application as stents for tracheal stenosis and spring for the treatment of craniosynostosis. From the positive results presented in other biomedical applications comes the possibility of investigating the application of this material as scaffold for tissue engineering (TE), acquiring a deeper knowledge about the polymeric blend by exploring a new processing technique while attending to the most fundamental demands of TE scaffolds. PLGA/PI was processed into randomly oriented microfibers through the dripping technique and submitted to physical-chemical and in vitro characterization. The production process of fibers did not show an effect over the polymer's chemical composition, despite the fact that PLGA and PI were observed to be immiscible. Mechanical assays reinforce the suitability of these scaffolds for soft tissue applications. Skeletal muscle cells demonstrated increases in metabolic activity and proliferation to the same levels of the control group. Human dermal fibroblasts didn't show the same behaviour, but presented cell growth with the same development profile as presented in the control group. It is plausible to believe that PLGA/PI fibrous three-dimensional scaffolds are suitable for applications in soft tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2581–2591, 2017.
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