Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture
H. P. Felgueiras
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
University of Minho, CT2M, Centre for Mechanical and Materials Technologies, Campus de Azurém, 4800-058 Guimarães, Portugal
Search for more papers by this authorL. Castanheira
University of Minho, CT2M, Centre for Mechanical and Materials Technologies, Campus de Azurém, 4800-058 Guimarães, Portugal
École Centrale de Paris, LGPM, Laboratoire de Génie des Procédés et Matériaux, Grande Voie des Vignes, 92295 Châtenay-Malabry, Paris, France
Search for more papers by this authorS. Changotade
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Search for more papers by this authorF. Poirier
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Search for more papers by this authorS. Oughlis
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Search for more papers by this authorM. Henriques
University of Minho, IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Campus de Gualtar, 4710-057 Braga, Portugal
Search for more papers by this authorC. Chakar
USJ, University Saint-Joseph, Faculty of Dentistry, Beirut, Lebanon
Search for more papers by this authorN. Naaman
USJ, University Saint-Joseph, Faculty of Dentistry, Beirut, Lebanon
Search for more papers by this authorR. Younes
USJ, University Saint-Joseph, Faculty of Dentistry, Beirut, Lebanon
Search for more papers by this authorV. Migonney
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Search for more papers by this authorJ. P. Celis
Catholic University of Leuven, MTM, Department of Metallurgy and Materials Engineering, 3001 Heverlee, Belgium
Search for more papers by this authorP. Ponthiaux
École Centrale de Paris, LGPM, Laboratoire de Génie des Procédés et Matériaux, Grande Voie des Vignes, 92295 Châtenay-Malabry, Paris, France
Search for more papers by this authorL. A. Rocha
University of Minho, CT2M, Centre for Mechanical and Materials Technologies, Campus de Azurém, 4800-058 Guimarães, Portugal
Search for more papers by this authorCorresponding Author
D. Lutomski
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Correspondence to: D. Lutomski (e-mail: [email protected])Search for more papers by this authorH. P. Felgueiras
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
University of Minho, CT2M, Centre for Mechanical and Materials Technologies, Campus de Azurém, 4800-058 Guimarães, Portugal
Search for more papers by this authorL. Castanheira
University of Minho, CT2M, Centre for Mechanical and Materials Technologies, Campus de Azurém, 4800-058 Guimarães, Portugal
École Centrale de Paris, LGPM, Laboratoire de Génie des Procédés et Matériaux, Grande Voie des Vignes, 92295 Châtenay-Malabry, Paris, France
Search for more papers by this authorS. Changotade
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Search for more papers by this authorF. Poirier
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Search for more papers by this authorS. Oughlis
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Search for more papers by this authorM. Henriques
University of Minho, IBB, Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Campus de Gualtar, 4710-057 Braga, Portugal
Search for more papers by this authorC. Chakar
USJ, University Saint-Joseph, Faculty of Dentistry, Beirut, Lebanon
Search for more papers by this authorN. Naaman
USJ, University Saint-Joseph, Faculty of Dentistry, Beirut, Lebanon
Search for more papers by this authorR. Younes
USJ, University Saint-Joseph, Faculty of Dentistry, Beirut, Lebanon
Search for more papers by this authorV. Migonney
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Search for more papers by this authorJ. P. Celis
Catholic University of Leuven, MTM, Department of Metallurgy and Materials Engineering, 3001 Heverlee, Belgium
Search for more papers by this authorP. Ponthiaux
École Centrale de Paris, LGPM, Laboratoire de Génie des Procédés et Matériaux, Grande Voie des Vignes, 92295 Châtenay-Malabry, Paris, France
Search for more papers by this authorL. A. Rocha
University of Minho, CT2M, Centre for Mechanical and Materials Technologies, Campus de Azurém, 4800-058 Guimarães, Portugal
Search for more papers by this authorCorresponding Author
D. Lutomski
Université Paris 13 Sorbonne Paris Cité, CSPBAT UMR CNRS 7244, Laboratoire de Biomatériaux et Polymères de Spécialité LBPS, UFR SMBH, 74, rue Marcel Cachin, 93017 Bobigny, Paris, France
Correspondence to: D. Lutomski (e-mail: [email protected])Search for more papers by this authorAbstract
The purpose of this study was to investigate the relationship between the osteoblastic cells behavior and biotribocorrosion phenomena on bioactive titanium (Ti). Ti substrates submitted to bioactive anodic oxidation and etching treatments were cultured up to 28 days with MG63 osteoblast-like cells. Important parameters of in vitro bone-like tissue formation were assessed. Although no major differences were observed between the surfaces topography (both rough) and wettability (both hydrophobic), a significant increase in cell attachment and differentiation was detected on the anodized substrates as product of favorable surface morphology and chemical composition. Alkaline phosphatase production has increased (≈20 nmol/min/mg of protein) on the anodized materials, while phosphate concentration has reached the double of the etched material and calcium production increased (over 20 µg/mL). The mechanical and biological stability of the anodic surfaces were also put to test through biotribocorrosion sliding solicitations, putting in evidence the resistance of the anodic layer and the cells capacity of regeneration after implant degradation. The Ti osteointegration abilities were also confirmed by the development of strong cell–biomaterial bonds at the interface, on both substrates. By combining the biological and mechanical results, the anodized Ti can be considered a viable option for dentistry. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 661–669, 2015.
REFERENCES
- 1Le Guehennec L, Soueidan A, Layrolle P, Amouriq Y. Surface treatments of titanium dental implants for rapid osseointegration. Dent Mater 2007; 23: 844–854.
- 2Whitters CJ, Strang R, Brown D, Clarke RL, Curtis RV, Hatton PV, Ireland AJ, Lloyd CH, McCabe JF, Nicholson JW, Scrimgeour SN, Setcos JC, Sherriff M, van Noort R, Watts DC, Wood D. Dental materials: 1997 literature review. J Dent 1999; 27: 401–435.
- 3Zhou ZR, Zeng J. Tribology of dental materials: A review. J Phys D: Appl Phys 2008; 41: 1–22.
- 4Zhu ZR, Yang D, Ma F. Investigation of a new design for zirconia dental implants. J Med Coll PLA 2007; 22: 303–311.
10.1016/S1000-1948(07)60062-8 Google Scholar
- 5Kasemo B, Lausma J. Metal selection and surface characteristics. In: Quintessence Publishing Co. I, editor. Tissue-Integrated Prostheses: Osseointegration in Clinical Dentistry; Quintessence Publishing, 1985. pp 99–116.
- 6Kuromoto NK, Simao RA, Soares GA. Titanium oxide films produced on commercially pure titanium by anodic oxidation with different voltages. Mater Charact 2007; 58: 114–121.
- 7Zhu X, Chen J, Scheideler L, Reichl R, Geis-Gerstorfer J. Effects of topography and composition of titanium surface oxides on osteoblast responses. Biomaterials 2004; 25: 4087–4103.
- 8Pourbaix M. Atlas of Electrochemical Equilibra in Aqueos Solutions, 2nd ed. USA: Nace TX; 1974.
- 9Carlsson L, Rostlund T, Albrektsson B, Albrektsson T, Branemark PI. Osseointegration of titanium implants. Acta Orthop Scand 1986; 57: 285–289.
- 10Branemark PI. Introduction to osseointegration. In: Quintessence Publishing Co. I, editor. Tissue-Integrated Prostheses: Osseointegration in Clinical Dentistry; Quintessence Publishing, 1985. pp 11–76.
- 11Krishna DSR, Brama YL, Sun Y. Thick rutile layer on titanium for tribological applications. Tribol Int 2007; 40: 329–334.
- 12Gabbi C, Cacchioli A, Ravanetti F, Spaggiari B, Borghetti P, Martini FM, et al. Osteogenesis and bone integration: The effect of new titanium surface treatments. Ann Fac Medic Vet di Parma 2005; 25: 307–318.
- 13Fini M, Cigada A, Rondelli G, Chiesa R, Giardino R, Giavarsi G, et al. In vitro and in vivo behaviour of Ca- and P- enriched anodized titanium. Biomaterials 1999; 20: 1587–1594.
- 14Franco RL, Chiesa R, Beloti MM, Oliveira PT, Rosa AL. Human osteoblastic cell response to Ca- and P- enriched titanium surface obtained by anodization. J Biomed Mater Res A 2009; 88: 841–848.
- 15Frauchiger VM, Schlottig F, Gasser B, Textor M. Anodic plasma-chemical treatment of CP titanium surfaces for biomedical applications. Biomaterials 2004; 25: 593–606.
- 16Das K, Bose S, Bandyopadhyay A. Surface modifications and cell-materials interactions with anodized Ti. Acta Biomater 2007; 3: 573–585.
- 17Souza JC, Henriques M, Oliveira R, Teughels W, Celis JP, Rocha LA. Do oral biofilms influence the wear and corrosion behavior of titanium? Biofouling 2010; 26: 471–478.
- 18Vieira A, Ribeiro A, Rocha L, Celis J-P. Influence of pH and corrosion inhibitors on the tribocorrosion of titanium in artificial saliva. Wear 2006; 261: 994–1001.
- 19Souza J, Barbosa S, Ariza E, Celis J-P, Rocha L. Simultaneous degradation by corrosion and wear of titanium in artificial saliva containing fluorides. Wear 2012; 292–293: 82–88.
- 20Squire MW, Ricci JL, Bizios R. Analysis of osteoblast mineral deposits on orthopaedic/dental implant metals. Biomaterials 1996; 17: 725–733.
- 21Van Oss CJ, Ju L, Chaudhury MK. Estimation of the polar parameters of the surface tension of liquids by contact angle measurements on gels. J Colloid Interface Sci 1989; 128: 313–319.
- 22Landolt D, Mischler S. Electrochemical methods in tribocorrosion: A critical appraisal. Electrochim Acta 2001; 46: 3913–3929.
- 23Feng B, Weng J, Yang BC, Qu SX, Zhang XD. Characterization of titanium surfaces with calcium and phosphate and osteoblast adhesion. Biomaterials 2004; 25: 3421–3428.
- 24Thirugnanam A, Kumar TSS, Chakkingal U. Bioactivity enhancement of commercial pure titanium by chemical treatments. Trends Biomater Art Organs 2009; 23: 76–85.
- 25Alves SA, Bayón R, Igartua A, Saénz de Viteri V, Rocha LA. Tribocorrosion behavior of anodic titanium oxide films produced by plasma electrolytic oxidation for dental implants. Lubrication Sci 2013. doi:10.1002/ls.1234.
- 26Lim YJ, Oshida Y. Initial contact angle measurements on variously treated dental/medical titanium materials. Biomed Mater Eng 2001; 11: 325–341.
- 27Lee JM, Lee JI, Lim YJ. In vitro investigation of anodization and CaP deposited titanium surface using MG63 osteoblast-like cells. Appl Surf Sci 2010; 256: 3086–3092.
- 28Mendonca G, Mendonca DB, Aragao FJ, Cooper LF. Advancing dental implant surface technology—From micron- to nanotopography. Biomaterials 2008; 29: 3822–3835.
- 29Zhu X, Chen J, Scheideler L, Altebaeumer T, Geis-Gerstorfer J, Kern D. Cellular reactions of osteoblasts to micron- and submicron-scale porous structures of titanium surfaces. Cells Tissues Organs 2004; 178: 13–22.
- 30Helary G, Noirclere F, Mayingi J, Migonney V. A new approach to graft bioactive polymer on titanium implants: Improvement of MG63 cell differentiation onto this coating. Acta Biomater 2009; 5: 124–133.
- 31Martin JY, Dean DD, Cochran DL, Simpson J, Boyan BD, Schwartz Z. Proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) cultured on previously used titanium surfaces. Clin Oral Implants Res 1996; 7: 27–37.
- 32Zhao G, Schwartz Z, Wieland M, Rupp F, Geis-Gerstorfer J, Cochran DL, et al. High surface energy enhances cell response to titanium substrate microstructure. J Biomed Mater Res A 2005; 74: 49–58.
- 33Zhu X, Kim KH, Jeong Y. Anodic oxide films containing Ca and P of titanium biomaterial. Biomaterials 2001; 22: 2199–2206.
- 34Higuchi C, Myoui A, Hashimoto N, Kurivama K, Yoshioka K, Yoshikawa H, Itoh K. Continuous inhibition of MAPK signalling promotes the eraly osteoblastic differentiation and mineralization of the extracellular matrix. J Bone Miner Res 2002; 17: 1785–1794.
- 35Kim KH, Ramaswaswamy N. Electrochemical surface modification of titanium in dentistry. Dental Mater J 2009; 28: 20–36.
- 36Larsson C, Thomsen P, Aronsson BO, Rodahl M, Lausmaa J, Kasemo B, et al. Bone response to surface-modified titanium implants: Studies on the early tissue response to machined and electropolished implants with different oxide thicknesses. Biomaterials 1996; 17: 605–616.
- 37Rodriguez R, Kim K, Ong JL. In vitro osteoblast response to anodized titanium and anodized titanium followed by hydrothermal treatment. J Biomed Mater Res A 2003; 65: 352–358.
- 38Craig RG, Powers JM. Restorative Dental Materials, 11th ed. USA: Mosby, Inc; 2002.
10.1111/j.1532-849X.2006.00112.x Google Scholar
- 39Deng H-W, Liu Y-Z. Current Topics in Bone biology. London: World Scientific Publishing; 2005.
- 40Kutz M. Biomedical Engineering and Design Handbook, 2nd ed. USA: McGraw Hill; 2009.
