Biomimetic chitosan–calcium phosphate composites with potential applications as bone substitutes: Preparation and characterization†
Constantin E. Tanase
Gh.Asachi' Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, Department of Chemical-Physics, 700050 Iasi, Romania
Search for more papers by this authorMarcel I. Popa
Gh.Asachi' Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, Department of Chemical-Physics, 700050 Iasi, Romania
Search for more papers by this authorCorresponding Author
Liliana Verestiuc
Gr.T.Popa' University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Science, Iasi, Romania
Gr.T.Popa' University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Science, Iasi, RomaniaSearch for more papers by this authorConstantin E. Tanase
Gh.Asachi' Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, Department of Chemical-Physics, 700050 Iasi, Romania
Search for more papers by this authorMarcel I. Popa
Gh.Asachi' Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection, Department of Chemical-Physics, 700050 Iasi, Romania
Search for more papers by this authorCorresponding Author
Liliana Verestiuc
Gr.T.Popa' University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Science, Iasi, Romania
Gr.T.Popa' University of Medicine and Pharmacy, Faculty of Medical Bioengineering, Department of Biomedical Science, Iasi, RomaniaSearch for more papers by this authorHow to cite this article: Tanase CE, Popa MI, Verestiuc L. 2012. Biomimetic chitosan-calcium phosphate composites with potential applications as bone substitutes: Preparation and characterization. J Biomed Mater Res Part B 2012:100B:700–708.
Abstract
A novel biomimetic technique for obtaining chitosan—calcium phosphates (Cs-CP) scaffolds are presented: calcium phosphates are precipitated from its precursors, CaCl2 and NaH2PO4 on the Cs matrix, under physiological conditions (human body temperature and body fluid pH; 37oC and pH = 7.2, respectively). Materials composition and structure have been confirmed by various techniques: elemental analysis, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM). FTIR and SEM data have shown the arrangement of the calcium phosphates-hydroxyapatite (CP-Hap) onto Cs matrix. In this case the polymer is acting as glue, bonding the calcium phosphates crystals. Behavior in biological simulated fluids (phosphate buffer solution—PBS and PBS—albumin) revealed an important contribution of the chelation between
and Ca2+ on the scaffold interaction with aqueous mediums; increased quantities of chitosan in composites permit the interaction with human albumin and improve the retention of fluid. The composites are slightly degraded by the lysozyme which facilitates an in vivo degradation control of bone substitutes. Modulus of elasticity is strongly dependent of the ratio chitosan/calcium phosphates and recommends the obtained biomimetic composites as promising materials for a prospective bone application. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
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