Enhancing Bone Regeneration: The Role of Biomimetic Silicified Collagen Scaffold in Osteogenesis and Angiogenesis
Ming-yuan Liu
Department of Orthodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China
Search for more papers by this authorYu-xuan Ma
State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China
Search for more papers by this authorLei Chen
Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
Search for more papers by this authorMeng Wang
Key School of Stomatology, Lanzhou University, Lanzhou, China
Search for more papers by this authorZheng-long Zhang
Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
Search for more papers by this authorCorresponding Author
Yu-xia Hou
Department of Orthodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China
Correspondence:
Yu-xia Hou ([email protected])
Li-na Niu ([email protected])
Search for more papers by this authorCorresponding Author
Li-na Niu
State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China
Correspondence:
Yu-xia Hou ([email protected])
Li-na Niu ([email protected])
Search for more papers by this authorMing-yuan Liu
Department of Orthodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China
Search for more papers by this authorYu-xuan Ma
State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China
Search for more papers by this authorLei Chen
Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
Search for more papers by this authorMeng Wang
Key School of Stomatology, Lanzhou University, Lanzhou, China
Search for more papers by this authorZheng-long Zhang
Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
Search for more papers by this authorCorresponding Author
Yu-xia Hou
Department of Orthodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China
Correspondence:
Yu-xia Hou ([email protected])
Li-na Niu ([email protected])
Search for more papers by this authorCorresponding Author
Li-na Niu
State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, China
Correspondence:
Yu-xia Hou ([email protected])
Li-na Niu ([email protected])
Search for more papers by this authorFunding: This work was supported by The National Natural Science Foundation of China. The National Clinical Research Center for Oral Diseases. Shaanxi Key Scientific and Technological Innovation Team.
The first two authors contributed equally to this article.
ABSTRACT
The identification of materials that effectively promote mineralization and vascularization is crucial for advancing clinical applications in bone regeneration. Biomimetic silicified collagen scaffold (SCS) has emerged as a promising candidate, demonstrating significant potential to enhance both osteogenesis and angiogenesis. However, the mechanisms by which SCS directly influences angiogenesis to facilitate bone defect healing remain largely unexplored. In this study, we observed that the implantation of SCS in rabbit femoral defects resulted in extensive bone regeneration and angiogenesis at the wound sites. Notably, SCS outperformed commercial alternatives such as Bio-Oss in terms of degradation and angiogenic response. In vitro assays further demonstrated that SCS upregulates angiogenic protein expression and promotes endothelial cell angiogenesis through the activation of the HIF-1α/VEGF signaling pathway. Consequently, SCS modulates the phenotype of vascular endothelial cells, leading to the formation of CD31hiEmcnhi type H endothelial cells, which are critical for effective bone regeneration. This study offers valuable perspectives on the dual effects of silicified materials on osteogenesis and angiogenesis, advancing the understanding of their potential functions in regenerative medicine.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| jbma37954-sup-0001-Supinfo.docxWord 2007 document , 206.5 KB |
Data S1. Supporting Information. |
| jbma37954-sup-0002-FigureS1.jpgJPEG image, 238.9 KB |
Figure SI-1. The dark blue spheres illustrate the infiltration of amorphous silica into the collagen fibers. Subsequently, the release of silicic acid is indicated by the lighter blue spheres, highlighting the dynamic process of silicification that influences bone metabolism. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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