3D bioprinted glioma microenvironment for glioma vascularization
Xuanzhi Wang
Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, China
Search for more papers by this authorXinda Li
Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing, China
Search for more papers by this authorJinju Ding
Center for Medical Device Evaluation, National Medical Products Administration, Beijing, China
Search for more papers by this authorXiaoyan Long
Department of research and development, East China Institute of Digital Medical Engineering, Shangrao, People's Republic of China
Search for more papers by this authorHaitao Zhang
Department of research and development, East China Institute of Digital Medical Engineering, Shangrao, People's Republic of China
Search for more papers by this authorXinzhi Zhang
Department of research and development, Medprin Regenerative Medical Technologies Co., Ltd, Shenzhen, People's Republic of China
Search for more papers by this authorCorresponding Author
Xiaochun Jiang
Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, China
Correspondence
Tao Xu, Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing 100084, China.
Email: [email protected]
Xiaochun Jiang, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu 241001, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Tao Xu
Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing, China
Department of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, China
Correspondence
Tao Xu, Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing 100084, China.
Email: [email protected]
Xiaochun Jiang, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu 241001, China.
Email: [email protected]
Search for more papers by this authorXuanzhi Wang
Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, China
Search for more papers by this authorXinda Li
Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing, China
Search for more papers by this authorJinju Ding
Center for Medical Device Evaluation, National Medical Products Administration, Beijing, China
Search for more papers by this authorXiaoyan Long
Department of research and development, East China Institute of Digital Medical Engineering, Shangrao, People's Republic of China
Search for more papers by this authorHaitao Zhang
Department of research and development, East China Institute of Digital Medical Engineering, Shangrao, People's Republic of China
Search for more papers by this authorXinzhi Zhang
Department of research and development, Medprin Regenerative Medical Technologies Co., Ltd, Shenzhen, People's Republic of China
Search for more papers by this authorCorresponding Author
Xiaochun Jiang
Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, China
Correspondence
Tao Xu, Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing 100084, China.
Email: [email protected]
Xiaochun Jiang, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu 241001, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Tao Xu
Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing, China
Department of Precision Medicine and Healthcare, Tsinghua-Berkeley Shenzhen Institute, Shenzhen, China
Correspondence
Tao Xu, Department of Mechanical Engineering, Biomanufacturing Center, Tsinghua University, Beijing 100084, China.
Email: [email protected]
Xiaochun Jiang, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu 241001, China.
Email: [email protected]
Search for more papers by this authorFunding information: Supported by Anhui Provincial Natural Science Foundation, Grant/Award Number: 2008085QH421; Key Scientific Research Project of Wannan Medical College, Grant/Award Number: WK2019ZF02; Special Scientific Research Fund for Talents Introduction in Yijishan Hospital of Wannan Medical College, Grant/Award Number: YR202008
Abstract
Glioblastoma is the most frequently diagnosed primary malignant brain tumor with unfavourable prognosis and high mortality. One of its key features is the extensive abnormal vascular network. Up to now, the mechanism of angiogenesis and the origin of tumor vascularization remain controversial. It is essential to establish an ideal preclinical tumor model to elucidate the mechanism of tumor vascularization, and the role of tumor cells in this process. In this study, both U118 cell and GSC23 cell exhibited good printability and cell proliferation. Compared with 3D-U118, 3D-GSC23 had a greater ability to form cell spheroids, to secrete vascular endothelial growth factor (VEGFA), and to form tubule-like structures in vitro. More importantly, 3D-glioma stem cells (GSC)23 cells had a greater power to transdifferentiate into functional endothelial cells, and blood vessels composed of tumor cells with an abnormal endothelial phenotype was observed in vivo. In summary, 3D bioprinted hydrogel scaffold provided a suitable tumor microenvironment (TME) for glioma cells and GSCs. This bioprinted model supported a novel TME for the research of glioma cells, especially GSCs in glioma vascularization and therapeutic targeting of tumor angiogenesis.
CONFLICTS OF INTEREST
The authors declare no potential conflict of interest.
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