Multichannel silk protein/laminin grafts for spinal cord injury repair
Qiang Zhang
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China
Department of Biomedical Engineering, Tufts University, Medford, 02155 Massachusetts
These authors contributed equally to this work.
Search for more papers by this authorShuqin Yan
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China
These authors contributed equally to this work.
Search for more papers by this authorRenchuan You
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China
Search for more papers by this authorDavid L. Kaplan
Department of Biomedical Engineering, Tufts University, Medford, 02155 Massachusetts
Search for more papers by this authorYu Liu
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
Search for more papers by this authorJing Qu
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
Search for more papers by this authorXiufang Li
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
Search for more papers by this authorCorresponding Author
Mingzhong Li
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
Correspondence to: M. Li; e-mail: [email protected]Search for more papers by this authorXin Wang
Centre for Advanced Materials and Performance Textiles, School of Fashion and Textiles, RMIT University, Melbourne, 3056 Victoria, Australia
Search for more papers by this authorQiang Zhang
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China
Department of Biomedical Engineering, Tufts University, Medford, 02155 Massachusetts
These authors contributed equally to this work.
Search for more papers by this authorShuqin Yan
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China
These authors contributed equally to this work.
Search for more papers by this authorRenchuan You
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073 China
Search for more papers by this authorDavid L. Kaplan
Department of Biomedical Engineering, Tufts University, Medford, 02155 Massachusetts
Search for more papers by this authorYu Liu
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
Search for more papers by this authorJing Qu
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
Search for more papers by this authorXiufang Li
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
Search for more papers by this authorCorresponding Author
Mingzhong Li
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123 China
Correspondence to: M. Li; e-mail: [email protected]Search for more papers by this authorXin Wang
Centre for Advanced Materials and Performance Textiles, School of Fashion and Textiles, RMIT University, Melbourne, 3056 Victoria, Australia
Search for more papers by this authorAbstract
The physical, chemical, and bioactive cues provided by biomaterials are critical for spinal cord regeneration following injury. In this study, we investigated the bioactivity of a silk-based scaffold for nerve tissue remodeling that featured morphological guidance in the form of ridges as well as bioactive molecules. Multichannel/laminin (LN) silk scaffolds stimulated growth, development, and the extension of primary hippocampal neurons after 7 days of culture in vitro. And then, the multichannel/LN silk scaffolds were implanted into 2-mm-long hemisection defects in Sprague-Dawley rat spinal cords for 70 days to evaluate their bioactivities of spinal cord remolding. Our results demonstrated that animal behavior was significantly improved in the multichannel/LN group, as evaluated by Basso–Beattie–Bresnahan score, whereas the implantation of multichannels and random pores groups resulted in recurring limps. Moreover, histology and immunohistochemical staining revealed an increase in blood vessels and expression of growth associated protein-43 and neurofilament-200 as well as reduced expression of glial fibrillary acidic protein in the multichannel/LN group, which contributed to the rebuilding of spinal cord defects. Thus, multichannel/LN silk scaffolds mediated cell migration, stimulated blood capillary formation, and promoted axonal extension, suggesting the utility of these scaffolds for spinal cord reconstruction. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3045–3057, 2016.
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