Monocyte preseeding leads to an increased implant bed vascularization of biphasic calcium phosphate bone substitutes via vessel maturation
Corresponding Author
M. Barbeck
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Correspondence to: M. Barbeck; e-mail: [email protected]Search for more papers by this authorR. E. Unger
Institute of Pathology, Repair-Lab, University Medical Center of the Johannes Gutenberg University, Mainz, Germany
Search for more papers by this authorP. Booms
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorE. Dohle
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorR. A. Sader
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorC. J. Kirkpatrick
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorS. Ghanaati
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorCorresponding Author
M. Barbeck
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Correspondence to: M. Barbeck; e-mail: [email protected]Search for more papers by this authorR. E. Unger
Institute of Pathology, Repair-Lab, University Medical Center of the Johannes Gutenberg University, Mainz, Germany
Search for more papers by this authorP. Booms
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorE. Dohle
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorR. A. Sader
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorC. J. Kirkpatrick
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorS. Ghanaati
Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt, Germany
Search for more papers by this authorAbstract
The present study analyzes the influence of the addition of monocytes to a biphasic bone substitute with two granule sizes (400–700 μm and 500–1000 μm). The majority of the added monocytes was detectable as mononuclear cells, while also low amounts of (chimeric) multinucleated giant cells (MNGCs) were found. No increase in the total number of MNGCs was established, but a significantly increased percent vascularization. Altogether, the results show that the added monocytes become involved in the tissue response to a biomaterial without marked changes in the overall reaction. Monocyte addition enables an increased implant bed vascularization especially via induction of vessel maturation and, thus intervenes positively in the healing reaction to a biomaterial. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2928–2935, 2016.
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