Melanocytes from the outer root sheath of human hair and epidermal melanocytes display improved melanotic features in the niche provided by cGEL, oligomer-cross-linked gelatin-based hydrogel
Katharina Sülflow
Saxon Incubator for Clinical Translation/Translational Centre for Regenerative Medicine, Leipzig University, Phillip-Rosenthal-Str.55, Leipzig, 04103 Germany
These authors contributed equally to this work.
Search for more papers by this authorMarie Schneider
Saxon Incubator for Clinical Translation/Translational Centre for Regenerative Medicine, Leipzig University, Phillip-Rosenthal-Str.55, Leipzig, 04103 Germany
These authors contributed equally to this work.
Search for more papers by this authorTina Loth
Leipzig University, Faculty of Biosciences Pharmacy and Psychology, Institute of Pharmacy Dept of Pharmaceutical Technology, Eilenburger Straße 15 a, 04317 Leipzig, Germany
Search for more papers by this authorChristian Kascholke
Leipzig University, Faculty of Biosciences Pharmacy and Psychology, Institute of Pharmacy Dept of Pharmaceutical Technology, Eilenburger Straße 15 a, 04317 Leipzig, Germany
Search for more papers by this authorMichaela Schulz-Siegmund
Leipzig University, Faculty of Biosciences Pharmacy and Psychology, Institute of Pharmacy Dept of Pharmaceutical Technology, Eilenburger Straße 15 a, 04317 Leipzig, Germany
Search for more papers by this authorMichael C. Hacker
Leipzig University, Faculty of Biosciences Pharmacy and Psychology, Institute of Pharmacy Dept of Pharmaceutical Technology, Eilenburger Straße 15 a, 04317 Leipzig, Germany
Search for more papers by this authorJan-Christoph Simon
Clinic and Policlinic for Dermatology, Venereology, and Allergology, Leipzig University Clinic, Faculty of Medicine, Leipzig, Germany
Search for more papers by this authorCorresponding Author
Vuk Savkovic
Saxon Incubator for Clinical Translation/Translational Centre for Regenerative Medicine, Leipzig University, Phillip-Rosenthal-Str.55, Leipzig, 04103 Germany
Correspondence to: Vuk Savkovic; Leipzig University, Saxon Incubator for Clinical Translation/Translational Centre for Regenerative Medicine, Phillip-Rosenthal-Str.55, 04103 Leipzig, Germany; e-mail: [email protected]Search for more papers by this authorKatharina Sülflow
Saxon Incubator for Clinical Translation/Translational Centre for Regenerative Medicine, Leipzig University, Phillip-Rosenthal-Str.55, Leipzig, 04103 Germany
These authors contributed equally to this work.
Search for more papers by this authorMarie Schneider
Saxon Incubator for Clinical Translation/Translational Centre for Regenerative Medicine, Leipzig University, Phillip-Rosenthal-Str.55, Leipzig, 04103 Germany
These authors contributed equally to this work.
Search for more papers by this authorTina Loth
Leipzig University, Faculty of Biosciences Pharmacy and Psychology, Institute of Pharmacy Dept of Pharmaceutical Technology, Eilenburger Straße 15 a, 04317 Leipzig, Germany
Search for more papers by this authorChristian Kascholke
Leipzig University, Faculty of Biosciences Pharmacy and Psychology, Institute of Pharmacy Dept of Pharmaceutical Technology, Eilenburger Straße 15 a, 04317 Leipzig, Germany
Search for more papers by this authorMichaela Schulz-Siegmund
Leipzig University, Faculty of Biosciences Pharmacy and Psychology, Institute of Pharmacy Dept of Pharmaceutical Technology, Eilenburger Straße 15 a, 04317 Leipzig, Germany
Search for more papers by this authorMichael C. Hacker
Leipzig University, Faculty of Biosciences Pharmacy and Psychology, Institute of Pharmacy Dept of Pharmaceutical Technology, Eilenburger Straße 15 a, 04317 Leipzig, Germany
Search for more papers by this authorJan-Christoph Simon
Clinic and Policlinic for Dermatology, Venereology, and Allergology, Leipzig University Clinic, Faculty of Medicine, Leipzig, Germany
Search for more papers by this authorCorresponding Author
Vuk Savkovic
Saxon Incubator for Clinical Translation/Translational Centre for Regenerative Medicine, Leipzig University, Phillip-Rosenthal-Str.55, Leipzig, 04103 Germany
Correspondence to: Vuk Savkovic; Leipzig University, Saxon Incubator for Clinical Translation/Translational Centre for Regenerative Medicine, Phillip-Rosenthal-Str.55, 04103 Leipzig, Germany; e-mail: [email protected]Search for more papers by this authorAuthor Disclosure Statement: No competing financial interests exist.
Abstract
Non-invasively based cell treatments of depigmented skin disorders are largely limited by means of cell sampling as much as by their routes of application. Human melanocytes cultivated from the outer root sheath of hair follicle (HUMORS) are among the cell types that fit the non-invasive concept by being cultivated out of a minimal sample: hair root. Eventual implementation of HUMORS as a graft essentially depends on a choice of suitable biocompatible, biodegradable carrier that would mechanically and biologically support the cells as transient niche and facilitate their engraftment. Hence, the melanotic features of follicle-derived HUMORS and normal human epidermal melanocytes (NHEM) in engineered scaffolds based on collagen, the usual leading candidate for graft material for a variety of skin transplantation procedures were tested. Hydrogel named cGEL, an enzymatically degraded bovine gelatin chemically cross-linked with an oligomeric copolymer synthesized from pentaerythritol diacrylate monostearate (PEDAS), maleic anhydride (MA), and N-isopropylacrylamide (NiPAAm) or diacetone acrylamide (DAAm), was used. The cGEL provided a friendly three-dimensional (3D) cultivation environment for human melanocytes with increased melanin content of the 3D cultures in comparison to Collagen Cell Carrier® (CCC), a commercially available bovine decellularized collagen membrane, and electrospun polycaprolactone (PCL) matrices. One of the cGEL variants fostered not only a dramatic increase in melanin production but also a significant enhancement of melanotic gene PAX3, PMEL, TYR, and MITF expression in comparison to that of both CCC full-length collagen and PCL scaffolds, providing a clearly superior melanocyte niche that may be a suitable candidate for grafting carriers. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 3115–3126, 2016.
Supporting Information
Additional Supporting Information may be found in the online version of this article.
| Filename | Description |
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| jbma35832-sup-0001-suppfig1.tif10.1 MB | Supporting Information Figure 1. |
| jbma35832-sup-0002-suppfig2.tif14.6 MB | Supporting Information Figure 2. |
| jbma35832-sup-0003-suppfig3.tif3.5 MB | Supporting Information Figure 3. |
| jbma35832-sup-0004-supptable1.docx14.6 KB | Supporting Information Table 1. |
| jbma35832-sup-0005-supptable2.docx14.6 KB | Supporting Information Table 2. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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