Original Article
Surface bound VEGF mimicking peptide maintains endothelial cell proliferation in the absence of soluble VEGF in vitro
Guillaume Le Saux,
Laurent Plawinski,
Camila Parrot,
Sylvain Nlate,
Laurent Servant,
Martin Teichmann,
Thierry Buffeteau,
Marie-Christine Durrieu,
Corresponding Author
Guillaume Le Saux
University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600 France
Correspondence to: G. Le Saux; e-mail: [email protected] or M.-C. Durrieu; e-mail: [email protected]Search for more papers by this authorLaurent Plawinski
University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600 France
Search for more papers by this authorCamila Parrot
Equipe Labellisée Contre Le Cancer, F-33607 Pessac, France
INSERM, U869, ARNA Laboratory, Equipe Labellisée Contre Le Cancer, Bordeaux, F-33076 France
Search for more papers by this authorSylvain Nlate
University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600 France
Search for more papers by this authorLaurent Servant
University of Bordeaux, ISM, UMR 5255, Talence, F-33400 France
Search for more papers by this authorMartin Teichmann
Equipe Labellisée Contre Le Cancer, F-33607 Pessac, France
INSERM, U869, ARNA Laboratory, Equipe Labellisée Contre Le Cancer, Bordeaux, F-33076 France
Search for more papers by this authorThierry Buffeteau
University of Bordeaux, ISM, UMR 5255, Talence, F-33400 France
Search for more papers by this authorMarie-Christine Durrieu
University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600 France
Search for more papers by this authorGuillaume Le Saux,
Laurent Plawinski,
Camila Parrot,
Sylvain Nlate,
Laurent Servant,
Martin Teichmann,
Thierry Buffeteau,
Marie-Christine Durrieu,
Corresponding Author
Guillaume Le Saux
University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600 France
Correspondence to: G. Le Saux; e-mail: [email protected] or M.-C. Durrieu; e-mail: [email protected]Search for more papers by this authorLaurent Plawinski
University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600 France
Search for more papers by this authorCamila Parrot
Equipe Labellisée Contre Le Cancer, F-33607 Pessac, France
INSERM, U869, ARNA Laboratory, Equipe Labellisée Contre Le Cancer, Bordeaux, F-33076 France
Search for more papers by this authorSylvain Nlate
University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600 France
Search for more papers by this authorLaurent Servant
University of Bordeaux, ISM, UMR 5255, Talence, F-33400 France
Search for more papers by this authorMartin Teichmann
Equipe Labellisée Contre Le Cancer, F-33607 Pessac, France
INSERM, U869, ARNA Laboratory, Equipe Labellisée Contre Le Cancer, Bordeaux, F-33076 France
Search for more papers by this authorThierry Buffeteau
University of Bordeaux, ISM, UMR 5255, Talence, F-33400 France
Search for more papers by this authorMarie-Christine Durrieu
University of Bordeaux, CBMN, UMR 5248, Pessac, F-33600 France
Search for more papers by this authorAbstract
Continuous glucose monitoring is an efficient method for the management of diabetes and in limiting the complications induced by large fluctuations in glucose levels. For this, intravascular systems may assist in producing more reliable and accurate devices. However, neovascularization is a key factor to be addressed in improving their biocompatibility. In this scope, the perennial modification of the surface of an implant with the proangiogenic Vascular Endothelial Growth Factor mimic peptide (SVVYGLR peptide sequence) holds great promise. Herein, we report on the preparation of gold substrates presenting the covalently grafted SVVYGLR peptide sequence and their effect on HUVEC behavior. Effective coupling was demonstrated using XPS and PM-IRRAS. The produced surfaces were shown to be beneficial for HUVEC adhesion. Importantly, surface bound SVVYGLR is able to maintain HUVEC proliferation even in the absence of soluble VEGF. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1425–1436, 2016.
Supporting Information
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