Osteogenic differentiation and proliferation of bone marrow-derived mesenchymal stromal cells on PDLLA + BMP-2-coated titanium alloy surfaces
Marcel Haversath
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Search for more papers by this authorTobias Hülsen
Department of Orthopaedics, Medical Faculty, University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorCarolin Böge
Department of Orthopaedics, Medical Faculty, University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorTjark Tassemeier
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Search for more papers by this authorStefan Landgraeber
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Search for more papers by this authorMonika Herten
Department of Orthopaedics, Medical Faculty, University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorSebastian Warwas
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Search for more papers by this authorRüdiger Krauspe
Department of Orthopaedics, Medical Faculty, University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorCorresponding Author
Marcus Jäger
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Correspondence to: M. Jäger; Univ.-Prof. Dr. med. Marcus Jäger, Hufelandstraße 55, 45147 Essen, Germany; e-mail: [email protected]Search for more papers by this authorMarcel Haversath
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Search for more papers by this authorTobias Hülsen
Department of Orthopaedics, Medical Faculty, University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorCarolin Böge
Department of Orthopaedics, Medical Faculty, University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorTjark Tassemeier
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Search for more papers by this authorStefan Landgraeber
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Search for more papers by this authorMonika Herten
Department of Orthopaedics, Medical Faculty, University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorSebastian Warwas
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Search for more papers by this authorRüdiger Krauspe
Department of Orthopaedics, Medical Faculty, University Düsseldorf, Düsseldorf, Germany
Search for more papers by this authorCorresponding Author
Marcus Jäger
Department of Orthopaedics and Trauma Surgery, University Duisburg-Essen, Essen, Germany
Correspondence to: M. Jäger; Univ.-Prof. Dr. med. Marcus Jäger, Hufelandstraße 55, 45147 Essen, Germany; e-mail: [email protected]Search for more papers by this authorDisclosures: The authors declare that there are no conflicts of interest.
Abstract
RhBMP-2 is clinically applied to enhance bone healing and used in combination with titanium fixation implants. The purpose of this in vitro study was to compare the osteogenic differentiation and proliferation of hMSC on native polished versus sandblasted titanium surfaces (TS) and to test their behavior on pure poly-D,L-lactide (PDLLA) coated as well as PDLLA + rhBMP-2 coated TS. Furthermore, the release kinetics of PDLLA + rhBMP-2-coated TS was investigated. Human bone marrow cells were obtained from three different donors (A: male, 16 yrs; B: male, 27 yrs, C: male, 49 yrs) followed by density gradient centrifugation and flow cytometry with defined antigens. The cells were seeded on native polished and sandblasted TS, PDLLA-coated TS and PDLLA + rhBMP-2-coated TS. Osteogenic differentiation (ALP specific activity via ALP and BCA assay) and proliferation (LDH cytotoxicity assay) was examined on day 7 and 14 and release kinetics of rhBMP-2 was investigated on day 3, 7, 10, and 14. We found significant higher ALP specific activity and LDH activity on native polished compared to native sandblasted surfaces. PDLLA led to decreased ALP specific and LDH activity on both surface finishes. Additional rhBMP-2 slightly diminished this effect. RhBMP-2-release from coated TS decreased nearly exponentially with highest concentrations at the beginning of the cultivation period. The results of this in vitro study suggest that native TS stimulate hMSC significantly stronger toward osteogenic differentiation and proliferation than rhBMP-2 + PDLLA-layered TS in the first 14 days of cultivation. The PDLLA-layer seems to inhibit local hMSC differentiation and proliferation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 145–154, 2016.
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