Dexamethasone released from cochlear implant coatings combined with a protein repellent hydrogel layer inhibits fibroblast proliferation
Corresponding Author
Antonina Wrzeszcz
Department of Otolaryngology, Hannover Medical School, 30625 Hannover, Germany
These authors contributed equally to this work.
Correspondence to: A. Wrzeszcz; e-mail: [email protected]Search for more papers by this authorBarbara Dittrich
Institute of Technical and Macromolecular Chemistry, Chair of Textile and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany
These authors contributed equally to this work.
Search for more papers by this authorDaniel Haamann
Institute of Technical and Macromolecular Chemistry, Chair of Textile and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany
Search for more papers by this authorPooyan Aliuos
Department of Otolaryngology, Hannover Medical School, 30625 Hannover, Germany
Search for more papers by this authorDoris Klee
Institute of Technical and Macromolecular Chemistry, Chair of Textile and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany
Search for more papers by this authorIngo Nolte
Small Animal Clinic, University of Veterinary Medicine Hannover, 30559 Hannover, Germany
Search for more papers by this authorThomas Lenarz
Department of Otolaryngology, Hannover Medical School, 30625 Hannover, Germany
Search for more papers by this authorGünter Reuter
Department of Otolaryngology, Hannover Medical School, 30625 Hannover, Germany
Search for more papers by this authorCorresponding Author
Antonina Wrzeszcz
Department of Otolaryngology, Hannover Medical School, 30625 Hannover, Germany
These authors contributed equally to this work.
Correspondence to: A. Wrzeszcz; e-mail: [email protected]Search for more papers by this authorBarbara Dittrich
Institute of Technical and Macromolecular Chemistry, Chair of Textile and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany
These authors contributed equally to this work.
Search for more papers by this authorDaniel Haamann
Institute of Technical and Macromolecular Chemistry, Chair of Textile and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany
Search for more papers by this authorPooyan Aliuos
Department of Otolaryngology, Hannover Medical School, 30625 Hannover, Germany
Search for more papers by this authorDoris Klee
Institute of Technical and Macromolecular Chemistry, Chair of Textile and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany
Search for more papers by this authorIngo Nolte
Small Animal Clinic, University of Veterinary Medicine Hannover, 30559 Hannover, Germany
Search for more papers by this authorThomas Lenarz
Department of Otolaryngology, Hannover Medical School, 30625 Hannover, Germany
Search for more papers by this authorGünter Reuter
Department of Otolaryngology, Hannover Medical School, 30625 Hannover, Germany
Search for more papers by this authorAbstract
The insertion of cochlear implants into the inner ear often causes inflammation and fibrosis inside the scala tympani and thus growth of fibrous tissue on the implant surface. This deposition leads to the loss of function in both electrical and laser-based implants. The design of this study was to realize fibroblast growth inhibition by dexamethasone (Dex) released from the base material of the implant [polydimethylsiloxane (PDMS)]. To prevent cell and protein adhesion, the PDMS was coated with a hydrogel layer [star-shaped polyethylene glycol prepolymer (sPEG)]. Drug release rates were studied over 3 months, and surface characterization was performed. It was observed that the hydrogel slightly smoothened the surface roughened by the Dex crystals. The hydrogel coating reduced and prolonged the release of the drug over several months. Unmodified, sPEG-coated, Dex-loaded, and Dex/sPEG-equipped PDMS filaments were cocultivated in vitro with fluorescent fibroblasts, analyzed by fluorescent microscopy, and quantified by cell counting. Compared to the unmodified PDMS, cell growth on all modified filaments was averagely 95% ±standard deviation (SD) less, while cell growth on the bottom of the culture dishes containing Dex-loaded filaments was reduced by 70% ±SD. Both, Dex and sPEG prevented direct cell growth on the filament surfaces, while drug delivery was maintained for the duration of several months. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 442–454, 2014.
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