Selection of highly osteogenic and chondrogenic cells from bone marrow stromal cells in biocompatible polymer-coated plates
G. Liu
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorK. Iwata
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorT. Ogasawara
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorJ. Watanabe
Department of Materials Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorK. Fukazawa
Department of Materials Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorK. Ishihara
Department of Materials Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Deparment of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorY. Asawa
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorY. Fujihara
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorU.-L. Chung
Deparment of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorT. Moro
Center of Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorY. Takatori
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorT. Takato
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorK. Nakamura
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorH. Kawaguchi
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorCorresponding Author
K. Hoshi
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, JapanSearch for more papers by this authorG. Liu
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorK. Iwata
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorT. Ogasawara
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorJ. Watanabe
Department of Materials Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorK. Fukazawa
Department of Materials Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorK. Ishihara
Department of Materials Engineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Deparment of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorY. Asawa
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorY. Fujihara
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorU.-L. Chung
Deparment of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorT. Moro
Center of Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorY. Takatori
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorT. Takato
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorK. Nakamura
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorH. Kawaguchi
Department of Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Search for more papers by this authorCorresponding Author
K. Hoshi
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, JapanSearch for more papers by this authorAbstract
To enrich the subpopulation that preserves self-renewal and multipotentiality from conventionally prepared bone marrow stromal cells (MSCs), we attempted to use 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer-coated plates that selected the MSCs with strong adhesion ability and evaluated the proliferation ability or osteogenic/chondrogenic potential of the MPC polymer-selected MSCs. The number of MSCs that were attached to the MPC polymer-coated plates decreased with an increase in the density of MPC unit (0–10%), whereas no significant difference in the proliferation ability was seen among these cells. The surface epitopes of CD29, CD44, CD105, and CD166, and not CD34 or CD45, were detectable in the cells of all MPC polymer-coated plates, implying that they belong to the MSC category. In the osteogenic and chondrogenic induction, the MSCs selected by the 2–5% MPC unit composition showed higher expression levels of osteoblastic and chondrocytic markers (COL1A1/ALP, or COL2A1/COL10A1/Sox9) at passage 2, compared with those of 0–1% or even 10% MPC unit composition, while the enhanced effects continued by passage 5. The selection based on the adequate cell adhesiveness by the MPC polymer-coated plates could improve the osteogenic and chondrogenic potential of MSCs, which would provide cell sources that can be used to treat the more severe and various bone/cartilage diseases. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010
References
- 1 Pittenger MF,Mackay AM,Beck SC,Jaiswal RK,Douglas R,Mosca JD,Moorman MA,Simonetti DW,Craig S,Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284: 143–147.
- 2 Makino S,Fukuda K,Miyoshi S,Konishi F,Kodama H,Pan J,Sano M,Takahashi T,Hori S,Abe H,Hata J,Umezawa A,Ogawa S. Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest 1999; 103: 697–705.
- 3 Kopen GC,Prockop DJ,Phinney DG. Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains. Proc Natl Acad Sci USA 1999; 96: 10711–10716.
- 4 Friedenstein AJ,Deriglasova UF,Kulagina NN,Panasuk AF,Rudakowa SF,Luria EA,Ruadkow IA. Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol 1974; 2: 83–92.
- 5 Sekiya I,Larson BL,Vuoristo JT,Cui JG,Prockop DJ. Adipogenic differentiation of human adult stem cells from bone marrow stroma (MSCs). J Bone Miner Res 2004; 19: 256–264.
- 6 Colter DC,Sekiya I,Prockop DJ. Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc Natl Acad Sci USA 2001; 98: 7841–7845.
- 7 Smith JR,Pochampally R,Perry A,Hsu SC,Prockop DJ. Isolation of a highly clonogenic and multipotential subfraction of adult stem cells from bone marrow stroma. Stem Cells 2004; 22: 823–831.
- 8 Ishii M,Koike C,Igarashi A,Yamanaka K,Pan H,Higashi Y,Kawaguchi H,Sugiyama M,Kamata N,Iwata T,Matsubara T,Nakamura K,Kurihara H,Tsuji K,Kato Y. Molecular markers distinguish bone marrow mesenchymal stem cells from fibroblasts. Biochem Biophys Res Commun 2005; 332: 297–303.
- 9 Pochampally RR,Smith JR,Ylostalo J,Prockop DJ. Serum deprivation of human marrow stromal cells (hMSCs) selects for a subpopulation of early progenitor cells with enhanced expression of OCT-4 and other embryonic genes. Blood 2004; 103: 1647–1652.
- 10 Ueda H,Watanabe J,Konno T,Takai M,Saito A,Ishihara K. Asymmetrically functional surface properties on biocompatible phospholipid polymer membrane for bioartificial kidney. J Biomed Mater Res A 2006; 77: 19–27.
- 11 Sawada S,Iwasaki Y,Nakabayashi N,Ishihara K. Stress response of adherent cells on a polymer blend surface composed of a segmented polyurethane and MPC copolymers. J Biomed Mater Res A 2006; 79: 476–484.
- 12 Sibarani J,Takai M,Ishihara K. Surface modification on microfluidic devices with 2-methacryloyloxyethyl phosphorylcholine polymers for reducing unfavorable protein adsorption. Colloids Surf B Biointerfaces 2007; 54: 88–93.
- 13 Lewis AL,Tolhurst LA,Stratford PW. Analysis of a phosphorylcholine-based polymer coating on a coronary stent pre- and post-implantation. Biomaterials 2002; 23: 1697–1706.
- 14 Kihara S,Yamazaki K,Litwak KN,Litwak P,Kameneva MV,Ushiyama H,Tokuno T,Borzelleca DC,Umezu M,Tomioka J,Tagusari O,Akimoto T,Koyanagi H,Kurosawa H,Kormos RL,Griffith BP. In vivo evaluation of a MPC polymer coated continuous flow left ventricular assist system. Artif Organs 2003; 27: 188–192.
- 15 Moro T,Takatori Y,Ishihara K,Konno T,Takigawa Y,Matsushita T,Chung UI,Nakamura K,Kawaguchi H. Surface grafting of artificial joints with a biocompatible polymer for preventing periprosthetic osteolysis. Nat Mater 2004; 3: 829–836.
- 16 Goda T,Ishihara K. Soft contact lens biomaterials from bioinspired phospholipid polymers. Expert Rev Med Devices 2006; 3: 167–174.
- 17 Kato Y,Iwamoto M,Koike T,Suzuki F,Takano Y. Terminal differentiation and calcification in rabbit chondrocyte cultures grown in centrifuge tubes: Regulation by transforming growth factor beta and serum factors. Proc Natl Acad Sci USA 1988; 85: 9552–9556.
- 18 Ebisawa K,Hata K,Okada K,Kimata K,Ueda M,Torii S,Watanabe H. Ultrasound enhances transforming growth factor β-mediated chondrocyte differentiation of human mesenchymal stem cells. Tissue Eng 2004; 10: 921–929.
- 19 Kuhl U,Ocalan M,Timpl R,von der Mark K. Role of laminin and fibronectin in selecting myogenic versus fibrogenic cells from skeletal muscle cells in vitro. Dev Biol 1986; 117: 628–635.
- 20 Roche P,Rousselle P,Lissitzky JC,Delmas PD,Malaval L. Isoform-specific attachment of osteoprogenitors to laminins: Mapping to the short arms of laminin-1. Exp Cell Res 1999; 250: 465–474.
- 21
Conget PA,Minguell JJ.
Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells.
J Cell Physiol
1999;
181:
67–73.
10.1002/(SICI)1097-4652(199910)181:1<67::AID-JCP7>3.0.CO;2-C CAS PubMed Web of Science® Google Scholar
- 22 Hall BM,Gibson LF. Regulation of lymphoid and myeloid leukemic cell survival: Role of stromal cell adhesion molecules. Leuk Lymphoma 2004; 45: 35–48.
- 23 Ip JE,Wu Y,Huang J,Zhang L,Pratt RE,Dzau VJ. Mesenchymal stem cells utilize integrin beta 1 not CXC chemokine receptor 4 for myocardial migration and engraftment. Mol Biol Cell 2007; 18: 2873–2882.
- 24 Ferrari G,Cusella-De Angelis G,Coletta M,Paolucci E,Stornaiuolo A,Cossu G,Mavilio F. Muscle regeneration by bone marrow-derived myogenic progenitors. Science 1998; 279: 1528–1530.
- 25 Horwitz EM,Prockop DJ,Fitzpatrick LA,Koo WW,Gordon PL,Neel M,Sussman M,Orchard P,Marx JC,Pyeritz RE,Brenner MK. Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta. Nat Med 1999; 5: 309–313.
- 26 Quarto R,Mastrogiacomo M,Cancedda R,Kutepov SM,Mukhachev V,Lavroukov A,Kon E,Marcacci M. Repair of large bone defects with the use of autologous bone marrow stromal cells. N Engl J Med 2001; 344: 385–386.
- 27 Vojtassak J,Danisovic L,Kubes M,Bakos D,Jarabek L,Ulicna M,Blasko M. Autologous biograft and mesenchymal stem cells in treatment of the diabetic foot. Neuro Endocrinol Lett 2006; 27( Suppl 2): 134–1371.
- 28 Fox JM,Chamberlain G,Ashton BA,Middleton J. Recent advances into the understanding of mesenchymal stem cell trafficking. Br J Haematol 2007; 137: 491–502.
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