Original Article

Comparative functional cell biological analysis of mesenchymal stem cells of the head and neck region: Potential impact on wound healing, trauma, and infection

Corresponding Author

Mark Jakob MD

Department of Otorhinolaryngology, University Hospital Bonn, Bonn, Germany

Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Bonn – Sigmund-Freud-Str. 25, 53105 Bonn, Germany. E-mail: [email protected]Search for more papers by this author

Hatim Hemeda PhD,

Hatim Hemeda PhD

Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany

Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany

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Kirsten Bruderek,

Kirsten Bruderek

Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany

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Andreas O. H. Gerstner MD,

Andreas O. H. Gerstner MD

Department of Otorhinolaryngology, University Hospital Bonn, Bonn, Germany

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Friedrich Bootz MD,

Friedrich Bootz MD

Department of Otorhinolaryngology, University Hospital Bonn, Bonn, Germany

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Stephan Lang MD,

Stephan Lang MD

Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany

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Sven Brandau PhD,

Sven Brandau PhD

Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany

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Mark Jakob MD,

Corresponding Author

Mark Jakob MD

Department of Otorhinolaryngology, University Hospital Bonn, Bonn, Germany

Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Bonn – Sigmund-Freud-Str. 25, 53105 Bonn, Germany. E-mail: [email protected]Search for more papers by this author

Hatim Hemeda PhD,

Hatim Hemeda PhD

Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany

Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany

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Kirsten Bruderek,

Kirsten Bruderek

Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany

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Andreas O. H. Gerstner MD,

Andreas O. H. Gerstner MD

Department of Otorhinolaryngology, University Hospital Bonn, Bonn, Germany

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Friedrich Bootz MD,

Friedrich Bootz MD

Department of Otorhinolaryngology, University Hospital Bonn, Bonn, Germany

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Stephan Lang MD,

Stephan Lang MD

Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany

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Sven Brandau PhD,

Sven Brandau PhD

Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany

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First published: 14 November 2012

https://doi.org/10.1002/hed.23196

Citations: 5

The results of this article were presented at the 82th annual meeting of the German ENT-Society in Freiburg, Germany, June 2011.

This project was supported in part by the Else–Kröner Fresenius Stiftung and the German Israeli Foundation.

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Abstract

Background

Mesenchymal stem cells (MSCs) are multipotent mesenchymal progenitor cells, originally identified in bone-marrow. Little is known about MSCs of the head and neck region. We investigated cell biological properties with a potential impact on wound healing of 2 different tissue-resident MSC populations.

Methods

MSCs were isolated from human nasal mucosa (nmMSCs) and parotid gland (pgMSCs). Clonogenic potential, cell surface markers, cytokine secretion, chemokine receptor expression, mobility, and adhesion to extracellular matrix were examined in unstimulated and stimulated MSCs.

Results

NmMSCs had the higher clonogenic potential. PgMSCs showed a broader panel of chemokine receptor expression and displayed higher mobility, especially after challenge with bacterial lipopolysaccharide (LPS). NmMSCs were less mobile and showed increased LPS-induced secretion of the inflammatory cytokine interleukin-8 (IL-8) compared with pgMSCs.

Conclusion

These data highlight functional differences between tissue-resident MSCs of the head and neck region, which may impact functional properties of these cells in response to trauma or infection. © 2012 Wiley Periodicals, Inc. Head Neck, 35: 1621–1629, 2013

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Volume35, Issue11

November 2013

Pages 1621-1629

Comparative functional cell biological analysis of mesenchymal stem cells of the head and neck region: Potential impact on wound healing, trauma, and infection

Abstract

Background

Methods

Results

Conclusion

REFERENCES

Citing Literature

References

Information

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Comparative functional cell biological analysis of mesenchymal stem cells of the head and neck region: Potential impact on wound healing, trauma, and infection

Abstract

Background

Methods

Results

Conclusion

REFERENCES

Citing Literature

References

Related

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