Volume 21, Issue 9 e202400842

Research Article

Phytochemical Analysis and Anti-dyslipidemia and Antioxidant Activities of Pluchea dioscoridis: In Vitro, In Silico and In Vivo Studies

Wageha S. Sultan

Department of Chemistry, Research Institute of Medicinal and Aromatic Plants, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Data curation (equal), Investigation (equal), Methodology (equal)

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Ayman M. Mahmoud,

Corresponding Author

Ayman M. Mahmoud

[email protected]

orcid.org/0000-0003-0279-6500

Department of Life Sciences, Faculty of Science & Engineering, Manchester Metropolitan University, Manchester, M1 5GD UK

Molecular Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Conceptualization (lead), Data curation (lead), Formal analysis (lead), Investigation (lead), Methodology (lead), Supervision (lead), Validation (lead), Visualization (lead), Writing - original draft (lead), Writing - review & editing (lead)

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Shimaa A. Ahmed,

Shimaa A. Ahmed

Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Data curation (equal), Investigation (equal), Methodology (equal)

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Reem S. Alruhaimi,

Reem S. Alruhaimi

Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671 Saudi Arabia

Contribution: Funding acquisition (lead), Investigation (supporting), Methodology (supporting), Resources (equal)

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Mohammed A. Alzoghaibi,

Mohammed A. Alzoghaibi

Physiology Department, College of Medicine, King Saud University, Riyadh, 11461 Saudi Arabia

Contribution: Investigation (supporting), Methodology (supporting), Resources (equal), Writing - review & editing (supporting)

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Ashraf A. El-Bassuony,

Ashraf A. El-Bassuony

Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Investigation (equal), Methodology (equal), Resources (equal), Supervision (supporting)

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Nabil A. Hasona,

Nabil A. Hasona

Biochemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Investigation (supporting), Methodology (equal), Supervision (supporting)

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Emadeldin M. Kamel,

Emadeldin M. Kamel

Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Conceptualization (supporting), Data curation (equal), Investigation (equal), Methodology (equal), Supervision (supporting), Writing - original draft (supporting)

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Wageha S. Sultan,

Wageha S. Sultan

Department of Chemistry, Research Institute of Medicinal and Aromatic Plants, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Data curation (equal), Investigation (equal), Methodology (equal)

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Ayman M. Mahmoud,

Corresponding Author

Ayman M. Mahmoud

[email protected]

orcid.org/0000-0003-0279-6500

Department of Life Sciences, Faculty of Science & Engineering, Manchester Metropolitan University, Manchester, M1 5GD UK

Molecular Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

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Shimaa A. Ahmed,

Shimaa A. Ahmed

Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Data curation (equal), Investigation (equal), Methodology (equal)

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Reem S. Alruhaimi,

Reem S. Alruhaimi

Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671 Saudi Arabia

Contribution: Funding acquisition (lead), Investigation (supporting), Methodology (supporting), Resources (equal)

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Mohammed A. Alzoghaibi,

Mohammed A. Alzoghaibi

Physiology Department, College of Medicine, King Saud University, Riyadh, 11461 Saudi Arabia

Contribution: Investigation (supporting), Methodology (supporting), Resources (equal), Writing - review & editing (supporting)

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Ashraf A. El-Bassuony,

Ashraf A. El-Bassuony

Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Investigation (equal), Methodology (equal), Resources (equal), Supervision (supporting)

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Nabil A. Hasona,

Nabil A. Hasona

Biochemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Investigation (supporting), Methodology (equal), Supervision (supporting)

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Emadeldin M. Kamel,

Emadeldin M. Kamel

Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, 62514 Egypt

Contribution: Conceptualization (supporting), Data curation (equal), Investigation (equal), Methodology (equal), Supervision (supporting), Writing - original draft (supporting)

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First published: 17 June 2024

https://doi.org/10.1002/cbdv.202400842

Citations: 1

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Abstract

Pluchea dioscoridis (L.) DC. is a flowering wild plant used traditionally in the treatment of rhematic disorders. This study investigated the phytochemical and in vitro radical scavenging activity (RSA), and in vivo anti-hyperlipidemic, antioxidant and anti-inflammatory properties of P. dioscoridis. The antihyperlipidemic efficacy was determined in a rat model of dyslipidemia. The extract and fractions of P. dioscoridis showed RSA with the ethyl acetate (EA) fraction exhibiting the most potent activity. The phytochemical analysis of P. dioscoridis EA fraction (PDEAF) led to the isolation of five compounds (lupeol, quercetin, lupeol acetate, stigmasterol, and syringic acid). To evaluate its anti-hyperlipidemic effect, three doses of PDEAF were supplemented to rats for 14 days and poloxamer-407 was administered on day 15 to induce dyslipidemia. All doses of PDEAF decreased plasma triglycerides, cholesterol, low-density lipoprotein-cholesterol (LDL−C) and very low-density lipoprotein-cholesterol (vLDL−C), and increased plasma lipoprotein lipase (LPL). PDEAF upregulated hepatic LDL receptor and suppressed 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, decreased lipid peroxidation and tumor necrosis factor (TNF)-α and enhanced reduced glutathione (GSH) and enzymatic antioxidants in dyslipidmeic rats. In silico findings revealed the binding affinity of the isolated compounds towards LPL, HMG-CoA reductase, and LDL receptor. In conclusion, P. dioscoridis is rich in phytoconstituents, exhibited RSA and its EA fraction effectively prevented acute dyslipidemia and its associated oxidative stress and inflammatory response.

Graphical Abstract

Conflict of Interests

The authors declare no conflict of interest.

Open Research

Data Availability Statement

The data that support the findings of this study are available in the supplementary material of this article.

Supporting Information

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Volume21, Issue9

September 2024

e202400842

Phytochemical Analysis and Anti-dyslipidemia and Antioxidant Activities of Pluchea dioscoridis: In Vitro, In Silico and In Vivo Studies

Abstract

Graphical Abstract

Conflict of Interests

Open Research

Data Availability Statement

Supporting Information

References

Citing Literature

References

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Phytochemical Analysis and Anti-dyslipidemia and Antioxidant Activities of Pluchea dioscoridis: In Vitro, In Silico and In Vivo Studies

Abstract

Graphical Abstract

Conflict of Interests

Open Research

Data Availability Statement

Supporting Information

References

Citing Literature

References

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