Guided Isolation of New Cytotoxic Cassane Diterpenoids from Caesalpinia sappan
Yue Jin
Department of Pharmacology, Mudanjiang Medical College, Mudanjiang, 157011 P. R. China
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016 P. R. China
These authors made equal contributions to the article.
Search for more papers by this authorZi-Wei Tong
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016 P. R. China
These authors made equal contributions to the article.
Search for more papers by this authorCorresponding Author
Hui-Yuan Gao
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016 P. R. China
Search for more papers by this authorCorresponding Author
Zhao-Hua Wu
Department of Pharmacology, Mudanjiang Medical College, Mudanjiang, 157011 P. R. China
Search for more papers by this authorYue Jin
Department of Pharmacology, Mudanjiang Medical College, Mudanjiang, 157011 P. R. China
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016 P. R. China
These authors made equal contributions to the article.
Search for more papers by this authorZi-Wei Tong
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016 P. R. China
These authors made equal contributions to the article.
Search for more papers by this authorCorresponding Author
Hui-Yuan Gao
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016 P. R. China
Search for more papers by this authorCorresponding Author
Zhao-Hua Wu
Department of Pharmacology, Mudanjiang Medical College, Mudanjiang, 157011 P. R. China
Search for more papers by this authorAbstract
Guided by an MS/MS-based molecular networking, six undescribed cassane diterpenoids and three known ones were isolated and identified from the seeds of Caesalpinia sappan. Their structures were unequivocally elucidated by extensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. Cytotoxic evaluation showed that phanginin JA exhibited significant antiproliferative activities against human non-small cell lung cancer (A549) cells with IC50 values of 16.79±0.83 μM. Further flow cytometry analysis revealed that phanginin JA could exert apoptotic effect of A549 cells by arresting cell cycle in G0/G1 phase.
Graphical Abstract
Conflict of interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available in the supplementary material of this article.
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