Zonal gene expression of chondrocytes in osteoarthritic cartilage
Corresponding Author
Naoshi Fukui
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Naoshi Fukui, Clinical Research Center, National Hospital Organization Sagamihara Hospital, Sakuradai 18-1, Sagamihara, Kanagawa 228-8522, Japan
Shiro Ikegawa, Laboratory for Bone and Joint Diseases, SNP Research Center, RIKEN, Shirokanedai 4-6-1, Minato-ku, Tokyo 108-8639, Japan
Search for more papers by this authorYasuko Ikeda
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorAtsuhiko Hikita
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorHiroshi Furukawa
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorHiroyuki Mitomi
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorNobuho Tanaka
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorYozo Katsuragawa
International Medical Center of Japan, Tokyo, Japan
Search for more papers by this authorSeizo Yamamoto
Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan
Search for more papers by this authorMotoji Sawabe
Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan
Search for more papers by this authorTakuo Juji
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorToshihito Mori
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorRyuji Suzuki
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorCorresponding Author
Shiro Ikegawa
SNP Research Center, RIKEN, Tokyo, Japan
Naoshi Fukui, Clinical Research Center, National Hospital Organization Sagamihara Hospital, Sakuradai 18-1, Sagamihara, Kanagawa 228-8522, Japan
Shiro Ikegawa, Laboratory for Bone and Joint Diseases, SNP Research Center, RIKEN, Shirokanedai 4-6-1, Minato-ku, Tokyo 108-8639, Japan
Search for more papers by this authorCorresponding Author
Naoshi Fukui
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Naoshi Fukui, Clinical Research Center, National Hospital Organization Sagamihara Hospital, Sakuradai 18-1, Sagamihara, Kanagawa 228-8522, Japan
Shiro Ikegawa, Laboratory for Bone and Joint Diseases, SNP Research Center, RIKEN, Shirokanedai 4-6-1, Minato-ku, Tokyo 108-8639, Japan
Search for more papers by this authorYasuko Ikeda
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorAtsuhiko Hikita
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorHiroshi Furukawa
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorHiroyuki Mitomi
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorNobuho Tanaka
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorYozo Katsuragawa
International Medical Center of Japan, Tokyo, Japan
Search for more papers by this authorSeizo Yamamoto
Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan
Search for more papers by this authorMotoji Sawabe
Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan
Search for more papers by this authorTakuo Juji
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorToshihito Mori
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorRyuji Suzuki
National Hospital Organization Sagamihara Hospital, Kanagawa, Japan
Search for more papers by this authorCorresponding Author
Shiro Ikegawa
SNP Research Center, RIKEN, Tokyo, Japan
Naoshi Fukui, Clinical Research Center, National Hospital Organization Sagamihara Hospital, Sakuradai 18-1, Sagamihara, Kanagawa 228-8522, Japan
Shiro Ikegawa, Laboratory for Bone and Joint Diseases, SNP Research Center, RIKEN, Shirokanedai 4-6-1, Minato-ku, Tokyo 108-8639, Japan
Search for more papers by this authorAbstract
Objective
To determine the chondrocyte metabolism in respective zones of osteoarthritic (OA) cartilage.
Methods
OA cartilage was obtained from macroscopically intact areas of 4 knee joints with end-stage OA. The cartilage was divided into 3 zones, and gene expression profiles were determined in the respective zones by a custom-designed microarray that focused on chondrocyte-related genes. For the genes whose expression was significantly different among the zones, the expression was compared between OA and control cartilage in the respective zones by an analysis using laser capture microdissection and real-time polymerase chain reaction (PCR). For some genes, the correlation of expression was investigated in specific cartilage zones.
Results
A total of 198 genes (∼40% of those investigated) were found to be expressed at significantly different levels among the zones. Expression of 26 of those genes was evaluated by laser capture microdissection and real-time PCR, which confirmed the validity of microarray analysis. The expression of cartilage matrix genes was mostly enhanced in OA cartilage, at similar levels across the zones but at different magnitudes among the genes. The expression of bone-related genes was induced either in the superficial zone or in the deep zone, and positive correlations were found among their expression in the respective zones. The expression of 5 proteinase genes was most enhanced in the superficial zone, where their expression was correlated, suggesting the presence of a common regulatory mechanism(s) for their expression.
Conclusion
In OA cartilage, the metabolic activity of chondrocytes differed considerably among zones. Characteristic changes were observed in the superficial and deep zones.
Supporting Information
Additional Supporting Information may be found in the online version of this article.
| Filename | Description |
|---|---|
| art_24036_sm_SupplTables.doc1.1 MB | Supplementary Tables 1A and 2 |
| art_24036_sm_SupplFig1.tif6 MB | Supplementary Figure 1. Correlation between the results of microarray analysis and real-time PCR. In order to validate the result of microarray, cDNA was synthesized from the RNA samples used for the microarray analysis, and the expression of 26 genes was evaluated by real-time PCR. For each gene, signal intensities of respective RNA samples acquired by the microarray are presented in a scattergram together with the expression ratios against GAPDH determined by real-time PCR. In each scattergram, result of linear regression analysis is also shown. Results of 13 cartilage matrix genes (A-M), 8 bone-related genes (N-U), and 5 proteinase genes (V-Z), are presented in the order of appearance in Figures 1-3. Signal intensities are shown in arbitrary unit. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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