Volume 136, Issue 15 47347

Article

Preparation of a modified crosslinked chitosan/polyvinyl alcohol blended affinity membrane for purification of His-tagged protein

Rong Zeng,

Rong Zeng

College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062 China

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Bu-Kun Jin,

Bu-Kun Jin

School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan, 430081 China

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Zhong-Hua Yang,

Corresponding Author

Zhong-Hua Yang

[email protected]

orcid.org/0000-0001-8341-4775

School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan, 430081 China

Correspondence to: Z.-H Yang (E-mail: [email protected]) and R. Guan (E-mail: [email protected])Search for more papers by this author

Rong Guan,

Corresponding Author

Rong Guan

[email protected]

College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062 China

Correspondence to: Z.-H Yang (E-mail: [email protected]) and R. Guan (E-mail: [email protected])Search for more papers by this author

Can Quan,

Can Quan

Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing, 102200 China

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Rong Zeng,

Rong Zeng

College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062 China

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Bu-Kun Jin,

Bu-Kun Jin

School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan, 430081 China

Search for more papers by this author

Zhong-Hua Yang,

Corresponding Author

Zhong-Hua Yang

[email protected]

orcid.org/0000-0001-8341-4775

School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan, 430081 China

Correspondence to: Z.-H Yang (E-mail: [email protected]) and R. Guan (E-mail: [email protected])Search for more papers by this author

Rong Guan,

Corresponding Author

Rong Guan

[email protected]

College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062 China

Correspondence to: Z.-H Yang (E-mail: [email protected]) and R. Guan (E-mail: [email protected])Search for more papers by this author

Can Quan,

Can Quan

Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing, 102200 China

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First published: 17 December 2018

https://doi.org/10.1002/app.47347

Citations: 11

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ABSTRACT

Based on a crosslinked chitosan (CS)/polyvinyl alcohol (PVA) matrix membrane, an immobilized metal ion affinity membrane (IMAM) using Cu²⁺ and Ni²⁺ ions as affinity ligands was prepared for purification of the His-tagged recombinant protein. The affinity membrane possessed a favorable membrane structure including 1.39 μm average pore size and 0.33 mL·cm⁻²·s⁻¹ water flux under 0.08 MPa pressure at 25 °C. The Cu²⁺ and Ni²⁺ ions capacities immobilized on the IMAM were 155.6 and 137.3 μmol·disk⁻¹, respectively. The IMAM had an excellent specific affinity to His-tagged protein. About 10-fold purification factor for the model protein was obtained in a batch adsorption, and serine hydroxymethyl transferase could be purified to a single band in sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis from its crude extract solution with an affinity membrane cartridge by a dynamic purification process. This work provides a promising IMAM for the purification of His-tagged recombinant proteins. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47347.

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Volume136, Issue15

April 15, 2019

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Preparation of a modified crosslinked chitosan/polyvinyl alcohol blended affinity membrane for purification of His-tagged protein

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Preparation of a modified crosslinked chitosan/polyvinyl alcohol blended affinity membrane for purification of His-tagged protein

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