Antifungal activity of chitosan against Fusarium oxysporum f. sp. cubense
M. Y. Al-Hetar
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Search for more papers by this authorCorresponding Author
M. A. Zainal Abidin
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia===Search for more papers by this authorM. Sariah
Institute of Tropical Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Search for more papers by this authorM. Y. Wong
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Search for more papers by this authorM. Y. Al-Hetar
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Search for more papers by this authorCorresponding Author
M. A. Zainal Abidin
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia===Search for more papers by this authorM. Sariah
Institute of Tropical Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Search for more papers by this authorM. Y. Wong
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia
Search for more papers by this authorAbstract
The in vitro antifungal activity of chitosan against Fusarium oxysporum f. sp. cubense Race 4 (FocR4) the causal agent of banana wilt was investigated. Chitosan at all concentrations tested reduced the hyphal growth of FocR4 on potato dextrose agar media and recording maximum inhibition of 76.36% at 8 mg/mL. The inhibitory effect was found to increase as chitosan concentration increases. The 50% effective concentration value was estimated by probit analysis, and it was 1.4 mg/mL. Chitosan was more effective in potato dextrose broth where it completely inhibited the mycelial growth of FocR4 at all concentrations tested. Chitosan inhibited the sporulation of FocR4 by a maximum of 96.53% at 8 mg/mL chitosan, and 100% inhibition for spore germination was recorded at all concentrations tested. Chitosan at concentrations of more than 1.6 mg/mL was also found to induce morphological changes in FocR4 characterized by agglomeration of hyphae, abnormal shapes, vesicles, or empty cells devoid of cytoplasm in the mycelia. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
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