Ferroptosis Detection: From Approaches to Applications
Fantian Zeng
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102 China
These authors contributed equally to this work.
Contribution: Conceptualization (equal), Writing - original draft (equal)
Search for more papers by this authorSureya Nijiati
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102 China
These authors contributed equally to this work.
Contribution: Conceptualization (equal), Writing - original draft (equal)
Search for more papers by this authorLongguang Tang
Affiliated Gaozhou People's Hospital, Guangdong Medical University, Guangdong, 524023 China
These authors contributed equally to this work.
Contribution: Writing - original draft (supporting)
Search for more papers by this authorJinmin Ye
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102 China
Contribution: Writing - original draft (supporting)
Search for more papers by this authorCorresponding Author
Zijian Zhou
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102 China
Shenzhen Research Institute of Xiamen University, Shenzhen, 518057 China
Contribution: Funding acquisition (lead), Supervision (lead), Writing - review & editing (equal)
Search for more papers by this authorCorresponding Author
Xiaoyuan Chen
Departments of Diagnostic Radiology, Chemical and Biomolecular Engineering, and Biomedical Engineering, Yong Loo Lin School of Medicine and College of Design and Engineering, National University of Singapore, Singapore, 119074 Singapore
Clinical Imaging Research Centre, Centre for Translational Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117599 Singapore
Nanomedicine Translational Research Program, NUS Center for Nanomedicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597 Singapore
Institute of Molecular and Cell Biology, Agency for Science, Technology, and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore, 138673 Singapore
Contribution: Funding acquisition (supporting), Supervision (supporting), Writing - review & editing (equal)
Search for more papers by this authorFantian Zeng
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102 China
These authors contributed equally to this work.
Contribution: Conceptualization (equal), Writing - original draft (equal)
Search for more papers by this authorSureya Nijiati
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102 China
These authors contributed equally to this work.
Contribution: Conceptualization (equal), Writing - original draft (equal)
Search for more papers by this authorLongguang Tang
Affiliated Gaozhou People's Hospital, Guangdong Medical University, Guangdong, 524023 China
These authors contributed equally to this work.
Contribution: Writing - original draft (supporting)
Search for more papers by this authorJinmin Ye
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102 China
Contribution: Writing - original draft (supporting)
Search for more papers by this authorCorresponding Author
Zijian Zhou
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, 361102 China
Shenzhen Research Institute of Xiamen University, Shenzhen, 518057 China
Contribution: Funding acquisition (lead), Supervision (lead), Writing - review & editing (equal)
Search for more papers by this authorCorresponding Author
Xiaoyuan Chen
Departments of Diagnostic Radiology, Chemical and Biomolecular Engineering, and Biomedical Engineering, Yong Loo Lin School of Medicine and College of Design and Engineering, National University of Singapore, Singapore, 119074 Singapore
Clinical Imaging Research Centre, Centre for Translational Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117599 Singapore
Nanomedicine Translational Research Program, NUS Center for Nanomedicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597 Singapore
Institute of Molecular and Cell Biology, Agency for Science, Technology, and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore, 138673 Singapore
Contribution: Funding acquisition (supporting), Supervision (supporting), Writing - review & editing (equal)
Search for more papers by this authorGraphical Abstract
We systematically summarize the ferroptosis detection approaches for subcellular organelles, biological events, and chemical markers, providing a multi-scale perspective on the regulation of the molecular machinery of ferroptosis. Moreover, we highlight the potential of advanced applications that integrate ferroptosis detection techniques with multifunctional platforms. The development of these applications holds great promise in promoting the transition of ferroptosis research from bench to bedside.
Abstract
Understanding the intricate molecular machinery that governs ferroptosis and leveraging this accumulating knowledge could facilitate disease prevention, diagnosis, treatment, and prognosis. Emerging approaches for the in situ detection of the major regulators and biological events across cellular, tissue, and in living subjects provide a multiscale perspective for studying ferroptosis. Furthermore, advanced applications that integrate ferroptosis detection and the latest technologies hold tremendous promise in ferroptosis research. In this review, we first briefly summarize the mechanisms and key regulators underlying ferroptosis. Ferroptosis detection approaches are then presented to delineate their design, mechanisms of action, and applications. Special interest is placed on advanced ferroptosis applications that integrate multifunctional platforms. Finally, we discuss the prospects and challenges of ferroptosis detection approaches and applications, with the aim of providing a roadmap for the theranostic development of a broad range of ferroptosis-related diseases.
Conflict of interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
Data sharing is not applicable to this article as no new data were created or analyzed in this study.
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