Protein NMR Resonance Assignment without Spectral Analysis: 5D SOlid-State Automated Projection SpectroscopY (SO-APSY)
Henry W. Orton
Research School of Chemistry, Australian National University, Canberra, ACT, 2601 Australia
These authors contributed equally to this work.
Search for more papers by this authorDr. Jan Stanek
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Faculty of Chemistry, University of Warsaw, 02089 Warsaw, Poland
These authors contributed equally to this work.
Search for more papers by this authorDr. Tobias Schubeis
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorDylan Foucaudeau
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorClaire Ollier
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorDr. Adrian W. Draney
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorDr. Tanguy Le Marchand
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorDr. Diane Cala-De Paepe
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorProf. Dr. Isabella C. Felli
CERM and Department of Chemistry, University of Florence, 50019 Sesto Fiorentino, Italy
Search for more papers by this authorProf. Dr. Roberta Pierattelli
CERM and Department of Chemistry, University of Florence, 50019 Sesto Fiorentino, Italy
Search for more papers by this authorProf. Dr. Sebastian Hiller
Biozentrum, University of Basel, 4056 Basel, Switzerland
Search for more papers by this authorDr. Wolfgang Bermel
Bruker BioSpin GmbH, Silberstreifen, 76287 Rheinstetten, Germany
Search for more papers by this authorCorresponding Author
Dr. Guido Pintacuda
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorHenry W. Orton
Research School of Chemistry, Australian National University, Canberra, ACT, 2601 Australia
These authors contributed equally to this work.
Search for more papers by this authorDr. Jan Stanek
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Faculty of Chemistry, University of Warsaw, 02089 Warsaw, Poland
These authors contributed equally to this work.
Search for more papers by this authorDr. Tobias Schubeis
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorDylan Foucaudeau
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorClaire Ollier
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorDr. Adrian W. Draney
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorDr. Tanguy Le Marchand
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorDr. Diane Cala-De Paepe
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorProf. Dr. Isabella C. Felli
CERM and Department of Chemistry, University of Florence, 50019 Sesto Fiorentino, Italy
Search for more papers by this authorProf. Dr. Roberta Pierattelli
CERM and Department of Chemistry, University of Florence, 50019 Sesto Fiorentino, Italy
Search for more papers by this authorProf. Dr. Sebastian Hiller
Biozentrum, University of Basel, 4056 Basel, Switzerland
Search for more papers by this authorDr. Wolfgang Bermel
Bruker BioSpin GmbH, Silberstreifen, 76287 Rheinstetten, Germany
Search for more papers by this authorCorresponding Author
Dr. Guido Pintacuda
Centre de Résonance Magnétique Nucléaire à Très Hauts Champs (FRE 2034 CNRS, UCBL, ENS Lyon), Université de Lyon, 69100 Villeurbanne, France
Search for more papers by this authorGraphical Abstract
Protein spins in a new dimension: The correlation of five resonance frequencies of nuclear spins within one NMR experiment is now feasible for rapidly rotating protein solids by using projection spectroscopy. The dramatically reduced ambiguity means that the assignment of backbone resonances of proteins can be obtained automatically without the need for manual spectral analysis.
Abstract
Narrow proton signals, high sensitivity, and efficient coherence transfers provided by fast magic-angle spinning at high magnetic fields make automated projection spectroscopy feasible for the solid-state NMR analysis of proteins. We present the first ultrahigh dimensional implementation of this approach, where 5D peak lists are reconstructed from a number of 2D projections for protein samples of different molecular sizes and aggregation states, which show limited dispersion of chemical shifts or inhomogeneous broadenings. The resulting datasets are particularly suitable to automated analysis and yield rapid and unbiased assignments of backbone resonances.
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