Volume 59, Issue 6 pp. 2392-2398
Research Article

In Vivo Solid-Phase Microextraction for Sampling of Oxylipins in Brain of Awake, Moving Rats

Alexander Napylov

Alexander Napylov

Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6 Canada

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Dr. Nathaly Reyes-Garces

Dr. Nathaly Reyes-Garces

Department of Chemistry, University of Waterloo, 200 University Avenue, Waterloo, ON, N2L 3G1 Canada

Current address: Restek Corporation, Bellefonte, PA, 16823 USA

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Dr. German Gomez-Rios

Dr. German Gomez-Rios

Department of Chemistry, University of Waterloo, 200 University Avenue, Waterloo, ON, N2L 3G1 Canada

Current address: Restek Corporation, Bellefonte, PA, 16823 USA

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Dr. Mariola Olkowicz

Dr. Mariola Olkowicz

Department of Chemistry, University of Waterloo, 200 University Avenue, Waterloo, ON, N2L 3G1 Canada

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Sofia Lendor

Sofia Lendor

Department of Chemistry, University of Waterloo, 200 University Avenue, Waterloo, ON, N2L 3G1 Canada

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Cian Monnin

Cian Monnin

Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6 Canada

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Dr. Barbara Bojko

Dr. Barbara Bojko

Department of Chemistry, University of Waterloo, 200 University Avenue, Waterloo, ON, N2L 3G1 Canada

Current address: Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland

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Dr. Clement Hamani

Dr. Clement Hamani

Neuroimaging Research Section, Centre for Addiction and Mental Health, 250 College Street, Toronto, ON, M5T 1R8 Canada

Harquail Centre for Neuromodulation, Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, 2075, Bayview Avenue, Toronto, ON, M4N 3M5 Canada

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Prof. Janusz Pawliszyn

Corresponding Author

Prof. Janusz Pawliszyn

Department of Chemistry, University of Waterloo, 200 University Avenue, Waterloo, ON, N2L 3G1 Canada

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Prof. Dajana Vuckovic

Corresponding Author

Prof. Dajana Vuckovic

Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6 Canada

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First published: 07 November 2019
Citations: 77

Graphical Abstract

Biocompatible in vivo solid-phase microextraction was used to measure 52 eicosanoids and other oxylipins directly in brains of awake moving rats with high temporal and spatial resolution. In vivo profiles of extracellular brain fluid did not reflect oxylipin concentrations in post-mortem tissue and provide a novel tool to elucidate oxylipin pathways and function.

Abstract

Oxylipins are key lipid mediators of important brain processes, including pain, sleep, oxidative stress, and inflammation. For the first time, an in-depth profile of up to 52 oxylipins can be obtained from the brains of awake moving animals using in vivo solid-phase microextraction (SPME) chemical biopsy tool in combination with liquid chromatography–high resolution mass spectrometry. Among these, 23 oxylipins are detectable in the majority of healthy wildtype samples. This new approach successfully eliminates the changes in oxylipin concentrations routinely observed during the analysis of post-mortem samples, allows time-course monitoring of their concentrations with high spatial resolution in specific brain regions of interest, and can be performed using the same experimental set-up as in vivo microdialysis (MD) thus providing a new and exciting tool in neuroscience and drug discovery.

Conflict of interest

The authors declare no conflict of interest.

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