Inhibition of Pathogen Adhesion by Bacterial Outer Membrane-Coated Nanoparticles
Dr. Yue Zhang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
These authors contributed equally to this work.
Search for more papers by this authorDr. Yijie Chen
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
These authors contributed equally to this work.
Search for more papers by this authorChristopher Lo
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorJia Zhuang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorDr. Pavimol Angsantikul
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorQiangzhe Zhang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorDr. Xiaoli Wei
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorZhidong Zhou
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorProf. Marygorret Obonyo
Department of Medicine, University of California San Diego, La Jolla, CA, 92093 USA
Search for more papers by this authorDr. Ronnie H. Fang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorDr. Weiwei Gao
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorCorresponding Author
Prof. Liangfang Zhang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorDr. Yue Zhang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
These authors contributed equally to this work.
Search for more papers by this authorDr. Yijie Chen
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
These authors contributed equally to this work.
Search for more papers by this authorChristopher Lo
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorJia Zhuang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorDr. Pavimol Angsantikul
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorQiangzhe Zhang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorDr. Xiaoli Wei
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorZhidong Zhou
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorProf. Marygorret Obonyo
Department of Medicine, University of California San Diego, La Jolla, CA, 92093 USA
Search for more papers by this authorDr. Ronnie H. Fang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorDr. Weiwei Gao
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorCorresponding Author
Prof. Liangfang Zhang
Department of NanoEngineering, Chemical Engineering Program, Moores Cancer Center, University of California San Diego, La Jolla, CA, 92039 USA
Search for more papers by this authorGraphical Abstract
Nanoparticles coated with bacterial outer membranes (denoted “OM-NPs”) are reported as an anti-adhesion nanomedicine that competes with source bacteria for binding sites and therefore inhibits bacterial adhesion to the host cells. Specifically, OM-NPs prepared with Helicobacter pylori outer membrane were demonstrated to inhibit bacterial binding to the gastric epithelial cells and stomach tissues.
Abstract
Anti-adhesion therapies interfere with the bacterial adhesion to the host and thus avoid direct disruption of bacterial cycles for killing, which may alleviate resistance development. Herein, an anti-adhesion nanomedicine platform is made by wrapping synthetic polymeric cores with bacterial outer membranes. The resulting bacterium-mimicking nanoparticles (denoted “OM-NPs”) compete with source bacteria for binding to the host. The “top-down” fabrication of OM-NPs avoids the identification of the adhesins and bypasses the design of agonists targeting these adhesins. In this study, OM-NPs are made with the membrane of Helicobacter pylori and shown to bind with gastric epithelial cells (AGS cells). Treatment of AGS cells with OM-NPs reduces H. pylori adhesion and such anti-adhesion efficacy is dependent on OM-NP concentration and its dosing sequence.
Supporting Information
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