Sequestration of Proteins by Fatty Acid Coacervates for Their Encapsulation within Vesicles
David Garenne
UMR 1332, biologie et pathologie du fruit, INRA, centre de Bordeaux, 33883 Villenave O'Ornon, France
Search for more papers by this authorDr. Laure Beven
UMR 1332, biologie et pathologie du fruit, INRA, centre de Bordeaux, 33883 Villenave O'Ornon, France
Search for more papers by this authorDr. Laurence Navailles
Université de Bordeaux, Centre de Recherche Paul-Pascal, CNRS, av. A. Schweitzer, 33600 Pessac, France
Search for more papers by this authorProf. Frédéric Nallet
Université de Bordeaux, Centre de Recherche Paul-Pascal, CNRS, av. A. Schweitzer, 33600 Pessac, France
Search for more papers by this authorDr. Erick J. Dufourc
Institute of Chemistry and Biology of Membranes and Nano-objects, UMR 5248, CNRS, université de Bordeaux, Institut polytechnique Bordeaux, 33600 Pessac, France
Search for more papers by this authorCorresponding Author
Dr. Jean-Paul Douliez
UMR 1332, biologie et pathologie du fruit, INRA, centre de Bordeaux, 33883 Villenave O'Ornon, France
Search for more papers by this authorDavid Garenne
UMR 1332, biologie et pathologie du fruit, INRA, centre de Bordeaux, 33883 Villenave O'Ornon, France
Search for more papers by this authorDr. Laure Beven
UMR 1332, biologie et pathologie du fruit, INRA, centre de Bordeaux, 33883 Villenave O'Ornon, France
Search for more papers by this authorDr. Laurence Navailles
Université de Bordeaux, Centre de Recherche Paul-Pascal, CNRS, av. A. Schweitzer, 33600 Pessac, France
Search for more papers by this authorProf. Frédéric Nallet
Université de Bordeaux, Centre de Recherche Paul-Pascal, CNRS, av. A. Schweitzer, 33600 Pessac, France
Search for more papers by this authorDr. Erick J. Dufourc
Institute of Chemistry and Biology of Membranes and Nano-objects, UMR 5248, CNRS, université de Bordeaux, Institut polytechnique Bordeaux, 33600 Pessac, France
Search for more papers by this authorCorresponding Author
Dr. Jean-Paul Douliez
UMR 1332, biologie et pathologie du fruit, INRA, centre de Bordeaux, 33883 Villenave O'Ornon, France
Search for more papers by this authorGraphical Abstract
Vesicular microreactors: Fatty acid based, membrane-free coacervates spontaneously sequester proteins and can reversibly form membranous vesicles upon changing the pH value, which leads to protein encapsulation within the vesicles. These micrometric capsules also provide a suitable environment for enzymatic reactions.
Abstract
Encapsulating biological materials in lipid vesicles is of interest for mimicking cells; however, except in some particular cases, such processes do not occur spontaneously. Herein, we developed a simple and robust method for encapsulating proteins in fatty acid vesicles in high yields. Fatty acid based, membrane-free coacervates spontaneously sequester proteins and can reversibly form membranous vesicles upon varying the pH value, the precrowding feature in coacervates allowing for protein encapsulation within vesicles. We then produced enzyme-enriched vesicles and show that enzymatic reactions can occur in these micrometric capsules. This work could be of interest in the field of synthetic biology for building microreactors.
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