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Site-Specific DNA Cleavage on a Solid Support: A Method for Mismatch Detection^†

Sandra Thoeni Dr.

Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056 Basel, Switzerland, Fax: (+41) 61-267-1105 http://www.chemie.unibas.ch/∼giese/index.html

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Christoph J. Kressierer Dr.,

Christoph J. Kressierer Dr.

Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056 Basel, Switzerland, Fax: (+41) 61-267-1105 http://www.chemie.unibas.ch/∼giese/index.html

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Bernd Giese Prof. Dr.,

Bernd Giese Prof. Dr.

[email protected]

Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056 Basel, Switzerland, Fax: (+41) 61-267-1105 http://www.chemie.unibas.ch/∼giese/index.html

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Sandra Thoeni Dr.,

Sandra Thoeni Dr.

Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056 Basel, Switzerland, Fax: (+41) 61-267-1105 http://www.chemie.unibas.ch/∼giese/index.html

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Christoph J. Kressierer Dr.,

Christoph J. Kressierer Dr.

Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056 Basel, Switzerland, Fax: (+41) 61-267-1105 http://www.chemie.unibas.ch/∼giese/index.html

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Bernd Giese Prof. Dr.,

Bernd Giese Prof. Dr.

[email protected]

Department of Chemistry, University of Basel, St. Johanns-Ring 19, 4056 Basel, Switzerland, Fax: (+41) 61-267-1105 http://www.chemie.unibas.ch/∼giese/index.html

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First published: 02 March 2007

https://doi.org/10.1002/anie.200603092

Citations: 4

^†

This work was supported by the Swiss National Science Foundation (NCCR Nanoscale Science).

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Graphical Abstract

Game, set, and mismatch: In a new method for the detection of base-pair mismatches, an immobilized DNA strand with a cleavage site (black square) and a detection tag (red circle) is hybridized with the target strand. If the target nucleotide has a mismatch, then the marker is released from the solid phase (see scheme).

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Volume46, Issue12

March 12, 2007

Pages 2112-2114

Site-Specific DNA Cleavage on a Solid Support: A Method for Mismatch Detection^†

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Site-Specific DNA Cleavage on a Solid Support: A Method for Mismatch Detection^†