[FeFe]-Hydrogenase
Kevin D Swanson
Montana State University, Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Bozeman, USA, MT 59717
Search for more papers by this authorDanillo O Ortillo
Montana State University, Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Bozeman, USA, MT 59717
Search for more papers by this authorJoan B Broderick
Montana State University, Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Bozeman, USA, MT 59717
Search for more papers by this authorJohn W Peters
Montana State University, Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Bozeman, USA, MT 59717
Search for more papers by this authorKevin D Swanson
Montana State University, Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Bozeman, USA, MT 59717
Search for more papers by this authorDanillo O Ortillo
Montana State University, Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Bozeman, USA, MT 59717
Search for more papers by this authorJoan B Broderick
Montana State University, Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Bozeman, USA, MT 59717
Search for more papers by this authorJohn W Peters
Montana State University, Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Bozeman, USA, MT 59717
Search for more papers by this authorAbstract
[FeFe]-Hydrogenases are metalloenzymes that are key components of the energy metabolism in many microbial communities. Hydrogenases function either to couple hydrogen oxidation to energy yielding processes or in proton reduction as a means to generate oxidized electron carriers required in fermentation. These enzymes have complex metal cluster with unique nonprotein derived ligands and are of significant interest due to their complexity and novel features as well as in biotechnology in developing superior hydrogen production methods. Biochemical, structural, and spectroscopic studies have provided significant insights into how these enzymes work and how these complex metal clusters key to their function are synthesized. This short report highlights some of the key features of the enzymes that have been elucidated through many years and the recent progress on [FeFe]-hydrogenase maturation pathway and synthesis of the active site H cluster.
3D Structure
[FeFe]-hydrogenases from (a) Clostridium pasteurianum PDB code 3C8Y, (b) Desulfovibrio desulfuricans 1HFE, and (c) Chlamydomonas reinhardtii 3LX4 generated in PyMOL.1 The active site H-cluster domain, the FS4A–FS4B domain, FS2 domain, and FS4C domain and the small subunit from D. desulfuricans are depicted in blue, green, light blue, purple, and yellow, respectively. Inorganic cofactors are represented by spheres, and Fe, S, C, N, and O are colored as rust, yellow, gray, red, and blue, respectively.
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