Fiber optic sensors have become indispensable tools for biomedical study because of their unique features such as (1) high sensitivity, (2) small footprint and endoscopic compatibility, (3) multiple agent distributive sensing capability, and (4) immunity from electromagnetic interference. In this chapter, we will provide a brief overview on the basic principle of fiber optic sensors, types of fiber optic sensors, and their applications to biomedical sensing. As a result of the page limitation, important work in this area may not be included in this chapter. Readers may find those contents in the listed reference material.