Proteins Containing Nonnatural Amino Acids
Part 7. Polyamides and Complex Proteinaceous Materials
Prof. Dr. Masahiko Sisido,
Prof. Dr. Masahiko Sisido
- [email protected]
- +81-86-251-8018 | Fax: +81-86-251-8219
Okayama University, Department of Bioscience and Biotechnology, 3-1-1 Tsushimanaka, Okayama, Japan, 700-8530
Search for more papers by this authorProf. Dr. Masahiko Sisido,
Prof. Dr. Masahiko Sisido
- [email protected]
- +81-86-251-8018 | Fax: +81-86-251-8219
Okayama University, Department of Bioscience and Biotechnology, 3-1-1 Tsushimanaka, Okayama, Japan, 700-8530
Search for more papers by this authorFirst published: 15 January 2005
Abstract
- Introduction
- Historical Outline
- Extension of Amino Acids
- Chemical Aminoacylation
- Extension of Codons (Four-base Codons)
- Adaptability of Four-base Codons in the Ribosome System and Extension to Five-base Codons
- Nonnatural Base Pairs for Codon Extension
- In-vitro Protein Synthesis in the Presence of tRNAs that are Amino-acylated with Nonnatural Amino Acids
- Purification and Identification of Nonnatural Mutants
- Multiple Incorporation of Nonnatural amino acids by using Different Orthogonal Four-base Codons
- Mutants that Contain a Single Nonnatural Amino Acid at Random Positions
- Combination of In-vitro Synthesis and Chemical Synthesis for Screening Nonnatural Mutants and for Large-scale Production
- In-vivo Synthesis of Nonnatural Mutants
- Examples of Specialty Functions of Nonnatural Mutants
- Fluorescence Labeling
- Electron Transfers Inside Protein Frameworks
- Outlook and Perspectives
- Acknowledgments
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