Carcinogenesis
Melatonin reduces the oxidation of nuclear DNA and membrane lipids induced by the carcinogen δ-aminolevulinic acid
Małgorzata Karbownik,
Dun-xian Tan,
Russel J. Reiter,
Małgorzata Karbownik
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, Texas, USA
Department of Thyroidology, Institute of Endocrinology, Medical University of Łódź, Łódź, Poland
Search for more papers by this authorDun-xian Tan
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, Texas, USA
Search for more papers by this authorCorresponding Author
Russel J. Reiter
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, Texas, USA
Department of Cellular and Structural Biology, University of Texas Health Science Center, Mail Code 7762, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA. Fax: 210-567-6948Search for more papers by this authorMałgorzata Karbownik,
Dun-xian Tan,
Russel J. Reiter,
Małgorzata Karbownik
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, Texas, USA
Department of Thyroidology, Institute of Endocrinology, Medical University of Łódź, Łódź, Poland
Search for more papers by this authorDun-xian Tan
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, Texas, USA
Search for more papers by this authorCorresponding Author
Russel J. Reiter
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, Texas, USA
Department of Cellular and Structural Biology, University of Texas Health Science Center, Mail Code 7762, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA. Fax: 210-567-6948Search for more papers by this authorFirst published: 23 August 2000
Citations: 39
Abstract
Well known are the anti-oxidant, free radical–scavenging and anti-tumorigenic properties of melatonin. δ-Aminolevulinic acid (ALA) is a precursor of heme synthesis. When over-produced and accumulated in tissues, ALA is a potential carcinogen, such as in the course of acute intermittent porphyria, hereditary tyrosinemia and lead poisoning. Our aim was to examine the potential protective effect of melatonin against oxidative damage to nuclear DNA and membrane lipids in rat lung and spleen caused by ALA. Changes in 8-hydroxy-2`-deoxyguanosine (8-OHdG) levels, an index of DNA damage, and the level of malondialdehyde + 4-hydroxyalkenals, an index of lipid peroxidation, were measured. Rats were injected with ALA (i.p., 40 mg/kg body weight, every other day) and/or with melatonin (i.p., 10 mg/kg body weight, 3 times daily) for 2 weeks. Both 8-OHdG and lipid peroxidation levels increased significantly in lung and spleen due to ALA treatment. Co-treatment with melatonin completely counteracted the effects of ALA. In conclusion, melatonin effectively protects nuclear DNA and lipids in rat lung and spleen against oxidative damage caused by the carcinogen ALA, and the indole may be of value as a supplement in patients suffering from molecular damage related to ALA accumulation. Int. J. Cancer 88:7–11, 2000. © 2000 Wiley-Liss, Inc.
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