Cold-stored platelets have better preserved contractile function in comparison with room temperature-stored platelets over 21 days
Corresponding Author
Prajeeda M. Nair
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Correspondence
Prajeeda M. Nair, Blood and Coagulation Research Department, US Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam Houston, San Antonio, TX 78234-4504, USA.
Email: [email protected]
Search for more papers by this authorMichael A. Meledeo
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorAdrienne R. Wells
Severe Burns Research Department, Combat Wound Repair Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorXiaowu Wu
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorJames A. Bynum
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorKai P. Leung
Severe Burns Research Department, Combat Wound Repair Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorBin Liu
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorAswathi Cheeniyil
Department of Biomedical Engineering, University of Texas at San Antonio, San Antonio, Texas, USA
Search for more papers by this authorAnand K. Ramasubramanian
Department of Biomedical Engineering, University of Texas at San Antonio, San Antonio, Texas, USA
Department of Chemical and Materials Engineering, San Jose State University, San Jose, California, USA
Search for more papers by this authorJohn W. Weisel
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
Search for more papers by this authorAndrew P. Cap
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorCorresponding Author
Prajeeda M. Nair
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Correspondence
Prajeeda M. Nair, Blood and Coagulation Research Department, US Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam Houston, San Antonio, TX 78234-4504, USA.
Email: [email protected]
Search for more papers by this authorMichael A. Meledeo
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorAdrienne R. Wells
Severe Burns Research Department, Combat Wound Repair Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorXiaowu Wu
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorJames A. Bynum
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorKai P. Leung
Severe Burns Research Department, Combat Wound Repair Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorBin Liu
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorAswathi Cheeniyil
Department of Biomedical Engineering, University of Texas at San Antonio, San Antonio, Texas, USA
Search for more papers by this authorAnand K. Ramasubramanian
Department of Biomedical Engineering, University of Texas at San Antonio, San Antonio, Texas, USA
Department of Chemical and Materials Engineering, San Jose State University, San Jose, California, USA
Search for more papers by this authorJohn W. Weisel
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
Search for more papers by this authorAndrew P. Cap
Blood and Coagulation Research Department, Combat Mortality Prevention Division, United States Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, Texas, USA
Search for more papers by this authorAbstract
Although it is well established that transfusion of platelets in cases of severe bleeding reduces mortality, the availability of platelets is hampered by harsh restrictions on shelf life due to elevated risks of microbial contamination and functional losses with room temperature-stored platelets (RTP) kept at 22°C. In contrast, many recent studies have shown that 4°C cold-stored platelets (CSP) are able to overcome these shortcomings leading to the recent Food and Drug Administration licensure for 14-day stored CSP when conventional platelets are unavailable. This work expands the evidence supporting superiority of CSP function by assaying the less explored platelet-mediated clot retraction of RTP and CSP in either autologous plasma (AP) or platelet additive solution (PAS) for up to 21 days. The results demonstrate that CSP have better preservation of contractile function, exhibiting retraction for up to 21 days in both AP and PAS and forming highly ordered fibrin scaffolds similar to those of fresh platelets. In contrast, RTP stored in AP showed impaired contractile function by Day 5 with no retraction after 10 days, whereas PAS-stored RTP retained contractile function for up to 21 days. Collectively, these findings support extended storage of CSP and suggest that storage in PAS can mitigate functional losses in RTP.
CONFLICT OF INTEREST
The authors have disclosed no conflicts of interest. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.
Supporting Information
| Filename | Description |
|---|---|
| trf16530-sup-0001-supinfo.docxWord 2007 document , 15.7 KB | Appendix S1 Supporting information |
| trf16530-sup-0002-FigureS1.pptxPowerPoint 2007 presentation , 48 KB | Figure S1 Platelets were collected in accordance with a US Army Institute of Surgical Research approved protocol. Apheresis platelets in PAS was prepared by diluting a hyperconcentrated platelet product to a final ratio of 65% T-PAS+ (Terumo BCT)/35% plasma through addition of T-PAS+ directly to the platelet bag after collection. For storage studies, 10 ml aliquots were obtained from the donor bag and sterilely transferred into 15-ml mini-bags (BCSI, Seattle, WA), with one minibag used for each time point and condition. The mini bags were stored for 5, 10, 15, or 21 days at 22°C (RTP) in an FDA-approved PLT incubator with agitation or at 4°C without agitation (CSP). Composition of T-PAS+ as per the manufacturer SDS is as follows: 0.030% magnesium chloride hexahydrate, 0.037% potassium chloride, 0.105% sodium dihydrogen phosphate dihydrate, 0.318% sodium citrate dihydrate, 0.405% sodium chloride, 0.442% sodium acetate trihydrate, 0.769% disodium hydrogen phosphate dodecahydrate. Further, number of donors per experiment is specified on the figure legends. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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