Acidic mammalian chitinase tuning after enteric helminths eradication in inflammatory respiratory disease patients
Corresponding Author
Marwa A. Hasby Saad
Medical Parasitology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Correspondence
Marwa A. Hasby Saad, Medical Parasitology Department, Tanta University, Faculty of Medicine, Egypt.
Email: [email protected]
Search for more papers by this authorMona Watany
Clinical Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorMohamed Tomoum
Otorhinolaryngeology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorDalia El-Mehy
Medical Parasitology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorMay Elsheikh
Paediatric Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorRagia Sharshar
Pulmonology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorCorresponding Author
Marwa A. Hasby Saad
Medical Parasitology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Correspondence
Marwa A. Hasby Saad, Medical Parasitology Department, Tanta University, Faculty of Medicine, Egypt.
Email: [email protected]
Search for more papers by this authorMona Watany
Clinical Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorMohamed Tomoum
Otorhinolaryngeology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorDalia El-Mehy
Medical Parasitology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorMay Elsheikh
Paediatric Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorRagia Sharshar
Pulmonology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
Search for more papers by this authorFunding information
We confirm that the current research did not receive a fund from any agency. However, we would like to express our great appreciation to our institute Tanta University Faculty of Medicine for providing our research with the basic equipments and kits.
Summary
Aim
This study aimed at investigating the presence of intestinal parasitic infections in inflammatory respiratory diseases patients during the disease attack, and measuring the acidic mammalian chitinase (AMCase) gene expression in blood before and after infection eradication.
Methodology
This case-control study included 123 inflammatory respiratory diseases patients and 120 apparently healthy individuals. Repeated stool examination was done, while total and specific IgE were measured. AMCase gene expression was analysed by real time-polymerase chain reaction (RT-PCR).
Results
Infection was detected in 32.5% of the diseased and 23.25% of the healthy individuals. Higher rate of the helminthic infection was detected (23.57) in comparison to the protozoal (12.19%) in the patients. A significantly higher rate of infection with the chitin-rich helminths “Enterobius vermicularis & Hymenolepis nana” and level of anti-Dermatophagoide-IgE were reported in the patients (14.63%, 6.5% and 23.57%, respectively). AMCase expression was significantly higher in helminths-infected patients than the noninfected, or protozoa infected. After infection eradication, AMCase expression significantly declined in the previously helminth-infected patients (mean ± SD = 13.9 ± 3.918 before and 4.515 ± 1.93 after), but insignificantly affected in the protozoa infected (mean ± SD = 2.095 ± .285 before and 2.675 ± 1.181 after).
Conclusion
Chitin-rich intestinal helminths are suspected to precipitate Th2-immune response in remote tissues by enhancing systemic AMCase expression through intestinal mucosa and macrophages irritation.
CONFLICT OF INTEREST
None.
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