Metabolomic differences of exhaled breath condensate among children with and without asthma
Ju Chang-Chien
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (lead), Formal analysis (lead), Investigation (equal), Methodology (equal), Project administration (supporting), Validation (lead), Writing - original draft (lead), Writing - review & editing (equal)
Search for more papers by this authorHsin-Yi Huang
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (supporting), Formal analysis (equal), Writing - review & editing (supporting)
Search for more papers by this authorHui-Ju Tsai
Institute of Population Health Sciences, National Health Research Institutes, Zhunan, Taiwan
Contribution: Conceptualization (supporting), Formal analysis (supporting), Investigation (supporting), Writing - review & editing (supporting)
Search for more papers by this authorChi-Jen Lo
Metabolomics Core Laboratory, Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan
Contribution: Data curation (supporting), Formal analysis (equal), Investigation (supporting), Methodology (lead), Software (equal), Validation (supporting), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorWan-Chen Lin
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (supporting), Investigation (supporting), Methodology (supporting), Writing - review & editing (supporting)
Search for more papers by this authorYu-Lun Tseng
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (supporting), Investigation (supporting), Methodology (supporting), Writing - review & editing (supporting)
Search for more papers by this authorShih-Ling Wang
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (supporting), Investigation (supporting), Methodology (supporting), Writing - review & editing (supporting)
Search for more papers by this authorHung-Yao Ho
Metabolomics Core Laboratory, Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan
Clinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital, Taoyuan, Taiwan
Department of Medical Biotechnology and Laboratory Science, Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Resources (supporting), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorCorresponding Author
Mei-Ling Cheng
Metabolomics Core Laboratory, Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan
Clinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital, Taoyuan, Taiwan
Department of Biomedical Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan
Correspondence
Tsung-Chieh Yao, Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, 5 Fu-Hsin Street, Kweishan, Taoyuan 33305, Taiwan.
Email: [email protected]
Mei-Ling Cheng, Department of Biomedical Sciences, Chang Gung University College of Medicine, 259 Wenhua 1st road, Kweishan, Taoyuan 33302, Taiwan.
Email: [email protected]
Contribution: Conceptualization (equal), Data curation (supporting), Formal analysis (supporting), Investigation (equal), Methodology (lead), Software (supporting), Validation (supporting), Writing - original draft (equal), Writing - review & editing (lead)
Search for more papers by this authorCorresponding Author
Tsung-Chieh Yao
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Community Medicine Research Center, Chang Gung Memorial Hospital at Keelung, Keelung, Taiwan
Correspondence
Tsung-Chieh Yao, Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, 5 Fu-Hsin Street, Kweishan, Taoyuan 33305, Taiwan.
Email: [email protected]
Mei-Ling Cheng, Department of Biomedical Sciences, Chang Gung University College of Medicine, 259 Wenhua 1st road, Kweishan, Taoyuan 33302, Taiwan.
Email: [email protected]
Contribution: Conceptualization (lead), Data curation (supporting), Funding acquisition (lead), Investigation (lead), Project administration (lead), Supervision (lead), Validation (supporting), Writing - original draft (lead), Writing - review & editing (lead)
Search for more papers by this authorJu Chang-Chien
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (lead), Formal analysis (lead), Investigation (equal), Methodology (equal), Project administration (supporting), Validation (lead), Writing - original draft (lead), Writing - review & editing (equal)
Search for more papers by this authorHsin-Yi Huang
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (supporting), Formal analysis (equal), Writing - review & editing (supporting)
Search for more papers by this authorHui-Ju Tsai
Institute of Population Health Sciences, National Health Research Institutes, Zhunan, Taiwan
Contribution: Conceptualization (supporting), Formal analysis (supporting), Investigation (supporting), Writing - review & editing (supporting)
Search for more papers by this authorChi-Jen Lo
Metabolomics Core Laboratory, Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan
Contribution: Data curation (supporting), Formal analysis (equal), Investigation (supporting), Methodology (lead), Software (equal), Validation (supporting), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorWan-Chen Lin
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (supporting), Investigation (supporting), Methodology (supporting), Writing - review & editing (supporting)
Search for more papers by this authorYu-Lun Tseng
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (supporting), Investigation (supporting), Methodology (supporting), Writing - review & editing (supporting)
Search for more papers by this authorShih-Ling Wang
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Data curation (supporting), Investigation (supporting), Methodology (supporting), Writing - review & editing (supporting)
Search for more papers by this authorHung-Yao Ho
Metabolomics Core Laboratory, Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan
Clinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital, Taoyuan, Taiwan
Department of Medical Biotechnology and Laboratory Science, Chang Gung University College of Medicine, Taoyuan, Taiwan
Contribution: Resources (supporting), Writing - original draft (supporting), Writing - review & editing (supporting)
Search for more papers by this authorCorresponding Author
Mei-Ling Cheng
Metabolomics Core Laboratory, Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan
Clinical Metabolomics Core Laboratory, Chang Gung Memorial Hospital, Taoyuan, Taiwan
Department of Biomedical Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan
Correspondence
Tsung-Chieh Yao, Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, 5 Fu-Hsin Street, Kweishan, Taoyuan 33305, Taiwan.
Email: [email protected]
Mei-Ling Cheng, Department of Biomedical Sciences, Chang Gung University College of Medicine, 259 Wenhua 1st road, Kweishan, Taoyuan 33302, Taiwan.
Email: [email protected]
Contribution: Conceptualization (equal), Data curation (supporting), Formal analysis (supporting), Investigation (equal), Methodology (lead), Software (supporting), Validation (supporting), Writing - original draft (equal), Writing - review & editing (lead)
Search for more papers by this authorCorresponding Author
Tsung-Chieh Yao
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan
Community Medicine Research Center, Chang Gung Memorial Hospital at Keelung, Keelung, Taiwan
Correspondence
Tsung-Chieh Yao, Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, 5 Fu-Hsin Street, Kweishan, Taoyuan 33305, Taiwan.
Email: [email protected]
Mei-Ling Cheng, Department of Biomedical Sciences, Chang Gung University College of Medicine, 259 Wenhua 1st road, Kweishan, Taoyuan 33302, Taiwan.
Email: [email protected]
Contribution: Conceptualization (lead), Data curation (supporting), Funding acquisition (lead), Investigation (lead), Project administration (lead), Supervision (lead), Validation (supporting), Writing - original draft (lead), Writing - review & editing (lead)
Search for more papers by this authorFunding information
This study was supported by the Ministry of Science and Technology of Taiwan (PIs: Yao, MOST 106-2314-B-182-051-MY3 and MOST 109-2314-B-182-042-MY3; Tsai, MOST 107-2314-B-400-031-MY3) or Chang Gung Medical Foundation (CMRPG3F1711 ~ 3F1713, CMRPG3E1201 ~ 3E1205, CMRPG3F0361, and CMRPG3J0121).
Abstract
Background
There remains an unmet need in objective tests for diagnosing asthma in children. The objective of this study was to investigate the potential of metabolomic profiles of exhaled breath condensate (EBC) to discriminate stable asthma in Asian children in the community.
Methods
One hundred and sixty-five Asian children (92 stable asthma and 73 non-asthmatic controls) participating in a population-based cohort were enrolled and divided into training and validation sets. Nuclear magnetic resonance-based metabolomic profiles of EBC samples were analyzed by using orthogonal partial least squares discriminant analysis.
Results
EBC metabolomic signature (lactate, formate, butyrate, and isobutyrate) had an area under the receiver operator characteristic curve (AUC) of 0.826 in discriminating children with and without asthma in the training set, which significantly outperformed FeNO (AUC = 0.574; P < .001) and FEV1/FVC % predicted (AUC = 0.569; P < .001). The AUC for EBC metabolomic signature was 0.745 in the validation set, which was slightly but not significantly lower than in the testing set (P = .282). We further extrapolated two potentially involved metabolic pathways, including pyruvate (P = 1.67 × 10−3; impact: 0.14) and methane (P = 1.89 × 10−3; impact: 0.15), as the most likely divergent metabolisms between children with and without asthma.
Conclusion
This study provided evidence supporting the role of EBC metabolomic signature to discriminate stable asthma in Asian children in the community, with a discriminative property outperforming conventional clinical tests such as FeNO or spirometry.
CONFLICT OF INTEREST
The authors declare no conflict of interest.
Supporting Information
| Filename | Description |
|---|---|
| pai13368-sup-0001-TableS1-S2.docxWord document, 34.8 KB | Table S1-S2 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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